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中国农学通报 ›› 2014, Vol. 30 ›› Issue (25): 97-103.doi: 10.11924/j.issn.1000-6850.2014-0693

所属专题: 生物技术 园艺

• 林学 园艺 园林 • 上一篇    下一篇

高温诱导叶用莴苣抽薹过程中内源激素含量变化分析

刘慧 郝敬虹 韩莹琰 刘超杰 苏贺楠   

  • 收稿日期:2014-03-14 修回日期:2014-03-21 出版日期:2014-09-05 发布日期:2014-09-05
  • 基金资助:
    国家自然科学基金委面上项目“叶用莴苣高温抽薹相关蛋白的鉴定及其互作分析”(31372057);北京市科学技术委员会项目“现代农业产业技术体系北京市叶类蔬菜创新团队建设专项”(BLVP-02);北京市教育委员会项目“科研基地建设-科技创新平台-蔬菜创新平台建设”(PXM2014_ 014207_000028);北京市教育委员会项目“科研基地建设——植物生产类研究生种业实验实习平台建设”(PXM2014_014207_000073);北京市教育委员会项目“基于蛋白质组学分析叶用莴苣高温抽薹的分子机制”(KM201310020014)。

Analysis of Endogenous Hormone Content During Bolting Caused by High Temperature in Lettuce

  • Received:2014-03-14 Revised:2014-03-21 Online:2014-09-05 Published:2014-09-05

摘要: 为探讨高温诱导叶用莴苣抽薹过程中内源激素的变化规律,进而揭示内源激素与抽薹的相互关系,以易抽薹叶用莴苣品种‘G-B30’为试材,利用植物生长箱模拟夏季高温,分析了高温诱导叶用莴苣抽薹过程中茎和叶的赤霉素(GA)、生长素(IAA)、茉莉酸甲酯(MeJA)、脱落酸(ABA)、玉米素核苷(ZR)、油菜素内酯(BR)含量的动态变化。结果表明,高温处理下,茎和叶在抽薹期(高温处理7天后)GA含量均显著高于抽薹前(高温处理前5天)。与对照相比,高温组茎中GA含量显著高于对照。高温处理组茎和叶中IAA、ABA、ZR含量抽薹期显著高于抽薹前,且高温组进入抽薹期时IAA、ABA含量也显著高于对照组。茎和叶BR含量表现为抽薹期显著低于抽薹前。与对照相比,高温组中抽薹期茎中BR含量明显降低而叶的BR含量无明显变化。高温处理组中茎和叶的MeJA含量均显著高于对照,且抽薹期显著低于抽薹前。综上所述,推测高水平的内源GA、IAA、ABA和ZR含量,低水平的BR可能促进叶用莴苣抽薹,而MeJA与叶用莴苣抽薹的关系不大。

关键词: 鉴定, 鉴定

Abstract: The contents of endogenous hormones including gibberellin (GA), indoleacetic acid (IAA), methyl jasmonate (MeJA), abscisic acid (ABA), ribosylzeatin (ZR), brassnioldie (BR) during bolting caused by high temperature in lettuce were determined by ELISA method to discuss their relation with bolting of lettuce. The results showed that GA content in stem and leaves of lettuce during bolting (after 7 days under the high temperature treatment) were significantly higher than before bolting (first 5 days under the high temperature treatment) under high temperature treatment. Compared with the control, GA content in stem were higher. In high temperature treatment, the contents of IAA, ABA, ZR during bolting were higher than before bolting. IAA and ABA were higher in high temperature treatment than the control, while the content of ZR in leaves was different. The content of BR was lower during bolting. Compared with the control, it showed no difference in leaves and significantly lower in stem. The concentration of MeJA in the high temperature treatment group was higher than in the control group and it’s significantly lower during bolting than before. These results indicated that high temperature could promote the bolting of lettuce, which might be connected with high level of endogenous GA, IAA, ABA, ZR and low level of BR, while the concentration of MeJA had no impact on bolting.