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中国农学通报 ›› 2014, Vol. 30 ›› Issue (29): 32-38.doi: 10.11924/j.issn.1000-6850.2014-1213

所属专题: 水产渔业

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

方斑东风螺地膜覆沙池和潮线下养殖系统细菌种群结构的PCR-DGGE 分析

李淑芳,邱德全,张继东,杨世平,邱明生   

  1. 广东海洋大学,广东海洋大学水产学院,广东海洋大学农学院,广东海洋大学水产学院,广东海洋大学水产学院
  • 收稿日期:2014-04-25 修回日期:2014-04-25 接受日期:2014-06-23 出版日期:2014-10-31 发布日期:2014-10-31
  • 通讯作者: 李淑芳
  • 基金资助:
    广东省海洋渔业局科技推广专项“工厂化养殖场、池塘、潮下限海区规模化养殖方斑东风螺;地膜覆盖沙池规模化养殖东风螺”(A201101I01;A201208E01);广东省科技计划项目“方斑东风螺规模化养殖的关键技术的研究与示范”(2012B020415006)。

PCR-DGGE Analysis of Bacterial Communities Structure in Babylonia areolata Culture Systems of The Subtidal Zone and The Pond Mulched Plastic Film and Sand in Bottom

  • Received:2014-04-25 Revised:2014-04-25 Accepted:2014-06-23 Online:2014-10-31 Published:2014-10-31

摘要: 为了解方斑东风螺养殖系统中细菌种群结构,研究和优化方斑东风螺地膜覆沙池养殖体系的管理模式。试验采用PCR-DGGE技术,对方斑东风螺地膜覆沙池养殖系统和潮线下养殖系统底质样品和水体样品中的细菌种群结构进行了分析。试验结果显示,以广东省湛江市东海岛海水为基础构建的方斑东风螺养殖系统内主要细菌种群包括变形菌门、绿弯菌门、蓝细菌门和放线菌门。其中地膜覆沙池养殖系统主要细菌种群包括γ-变形菌纲、α-变形菌纲、δ-变形菌纲、变形菌纲、厌氧绳菌纲、蓝细菌和放线菌等;潮线下养殖系统主要细菌种群包括γ-变形菌纲、α-变形菌纲、δ-变形菌纲、厌氧绳菌纲、蓝细菌,而变形菌纲和放线菌的分布较少。被检测的两个养殖系统中细菌种群均具有较高的多样性,UPGMA聚类分析结果表明,被检测的两个养殖系统底质样品S1 和S2 为一簇,种群结构相似度为54.5%;两个养殖系统的水体样品S3 和S4 为一簇,种群结构相似度为84.0%;试验结果说明,通过池塘水体更新和微生物种群监测等调控手段有效控制养殖系统微生物种群结构,能够使方斑东风螺地膜覆沙池养殖系统的微生物生态水平接近潮线下养殖系统。

关键词: 荚果蕨, 荚果蕨, 幼苗, 有机肥, 光合特性

Abstract: To know the bacterial communities structure in Babylonia areolata culture systems and to research and optimize the management pattern of Babylonia areolata culture systems of the pond mulched plastic film and sand in bottom, the bacterial communities in Babylonia areolata culture systems of the subtidal zone and the pond mulched plastic film and sand in bottom were analyzed at molecular level by adopting the denaturing gradient gel electrophoresis (DGGE). The results indicated that the dominant bacterial communities in Babylonia areolata culture systems of the subtidal zone and the pond mulched plastic film and sand in bottom, which were built on the basis of the seawater in East-island of Zhanjiang, included Proteobacteria Chloroflexi, Cyanobacteria and Actinobacteria. The dominant bacterial groups in the above pond culture system were Gammaproteobacteria, Alphaproteobacteria, Deltaproteobacteria, Epsilonproteobacteria, Anaerolineae, Cyanobacteria and Actinobacteria. The dominant bacterial communities in the subtidal zone culture system were Gammaproteobacteria, Alphaproteobacteria, Deltaproteobacteria, Anaerolineae and Cyanobacteria, and there were less Epsilonproteobacteria and Actinobacteria in the culture system. The higher diversity was detected in the above two culture systems. The results of unweighted pair group method with arithmetic average (UPGMA) showed that the bacterial communities of the sediment samples S1 and S2 in the above two culture systems were a cluster, the similarity of bacterial communities was 54.5% . The bacterial communities of seawater samples S3 and S4 in the above culture systems were a cluster, the similarity of the bacterial communities was 84.0%. The results showed that the microorganism ecological level in the Babylonia areolata culture systems of the pond mulched plastic film and sand in bottom could be similar to the subtidal zone culture systems through changing the pond seawater and monitoring the microbial population.