贪铜菌6-5双组分信号系统czcR2-czcS2 的克隆

doi:10.11924/j.issn.1000-6850.2014-1865

中国农学通报 ›› 2014, Vol. 30 ›› Issue (36): 148-154.doi: 10.11924/j.issn.1000-6850.2014-1865

所属专题：生物技术

贪铜菌6-5双组分信号系统czcR2-czcS2 的克隆

单世平,郭照辉,张德元,刘清术,程伟,黄军,魏小武,王玉双,伍善东,付祖姣

(湖南省微生物研究院,长沙 410009）

收稿日期:2014-07-05 修回日期:2015-01-09 接受日期:2014-08-08 出版日期:2015-03-20 发布日期:2015-03-20
通讯作者: 单世平
基金资助:
湖南省自然科学基金项目“贪铜菌6-5富集土壤中重金属镉的分子机理研究”(13JJ2035)；湖南省“一化四体系”专项资金项目“降低水稻镉吸收原位固定修复技术研究及应用示范”(2014年)

Gene Cloning and Bioinformatics Analysis of Two-Component Signal Systems
czcR2-czcS2 from Cupriavidus 6-5

Shan Shiping, Guo Zhaohui, Zhang Deyuan, Liu Qingshu, Cheng Wei, Huang Jun, Wei Xiaowu,Wang Yushuang, Wu Shandong, Fu Zujiao

(Hunan Academy of Microbiology, Changsha 410009)

Received:2014-07-05 Revised:2015-01-09 Accepted:2014-08-08 Online:2015-03-20 Published:2015-03-20

摘要/Abstract

摘要：

为阐明贪铜菌6-5的耐镉机理，更有效的治理土壤重金属污染。本研究利用同源序列克隆技术从本研究室筛选的高耐镉菌株贪铜菌6-5中成功克隆出双组分信号转导系统czcR2-czcS2，通过DNA测序及序列比对，结果表明，该扩增产物的DNA序列与耐金属贪铜菌CH34中megaplasmid质粒的czcR2和czcS2基因的同源性分别为99%和98%。czcR2-czcS2彼此相邻，并且转录方向一致，很可能作为一个独立的操纵子控制其他功能基因的表达。并利用生物信息学对该调控基因的一般特性、跨膜区和信号肽进行了预测，该研究为开展更为深入的研究以及制定有针对性的重金属污染治理对策建立了基础，具有重要的理论意义和实际应用潜能。

关键词: 白肋烟, 白肋烟, 增温排湿, 质量

Abstract: In order to elucidate the Cd-resistance mechanism of Cupriavidus 6-5 and reduce the heavy metal pollution more efficiently. The two-component signal transduction system czcR2-czcS2 from high Cd- resistance strain Cupriavidus 6-5 was successfully cloned by homology-based cloning method. Results of DNA sequencing and sequence alignment showed that the identity of the nucleotide sequence of obtained genes czcR2 and czcS2 between Cupriavidus 6-5 and the megaplasmid of Cupriavidus metallidurans CH34 were from 99% and 98%, respectively. czcR2 and czcS2, adjacent to each other and the same direction of transcription, control other functional gene expression probably as an independent operon. The general characteristics, signal peptide and transmembrane domain of the regulatory gene were predicted by the bioinformatics. The study establish foundation for the development of more thorough research and making of heavy metal pollution control countermeasure, the work has the theory significance and actual application potential.

单世平,郭照辉,张德元,刘清术,程伟,黄军,魏小武,王玉双,伍善东,付祖姣. 贪铜菌6-5双组分信号系统czcR2-czcS2 的克隆[J]. 中国农学通报, 2014, 30(36): 148-154.

Shan Shiping,Guo Zhaohui,Zhang Deyuan,Liu Qingshu,Cheng Wei,Huang Jun,Wei Xiaowu,Wang Yushuang,Wu Shandong and Fu Zujiao. Gene Cloning and Bioinformatics Analysis of Two-Component Signal Systems
czcR2-czcS2 from Cupriavidus 6-5[J]. Chinese Agricultural Science Bulletin, 2014, 30(36): 148-154.

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贪铜菌6-5双组分信号系统czcR2-czcS2 的克隆

Gene Cloning and Bioinformatics Analysis of Two-Component Signal Systems
czcR2-czcS2 from Cupriavidus 6-5

可视化

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贪铜菌6-5双组分信号系统czcR2-czcS2 的克隆

Gene Cloning and Bioinformatics Analysis of Two-Component Signal SystemsczcR2-czcS2 from Cupriavidus 6-5

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Gene Cloning and Bioinformatics Analysis of Two-Component Signal Systems
czcR2-czcS2 from Cupriavidus 6-5