水稻恢复系‘成恢177’稻瘟病抗性与遗传背景分析

doi:10.11924/j.issn.1000-6850.2014-2356

中国农学通报 ›› 2015, Vol. 31 ›› Issue (9): 31-39.doi: 10.11924/j.issn.1000-6850.2014-2356

所属专题：水稻；农业生态

水稻恢复系‘成恢177’稻瘟病抗性与遗传背景分析

任鄄胜¹,陆贤军¹,高方远¹,彭云良²,吴贤婷¹,苏相文¹,罗正良¹,张雪梅²,任光俊¹

(¹四川省农业科学院作物研究所,成都 610066；²四川省农业科学院植物保护研究所,成都 610066）

收稿日期:2014-08-28 修回日期:2015-03-03 接受日期:2014-09-29 出版日期:2015-04-07 发布日期:2015-04-07
通讯作者: 任光俊
基金资助:
国家“863”项目“水稻分子设计育种技术与品种创制”(2012AA101101)；四川省青年科技创新研究团队专项计划资助项目“绿色超级稻新品种培育”(2011JTD0022)。

Analysis of Blast Resistance and Genetic Background in Rice Restorer ‘Chenghui 177’

Ren Juansheng¹, Lu Xianjun¹, Gao Fangyuan¹, Peng Yunliang², Wu Xianting¹,

Su Xiangwen¹, Luo Zhengliang¹, Zhang Xuemei², Ren Guangjun¹(¹Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066;²Plant Protection Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066)

Received:2014-08-28 Revised:2015-03-03 Accepted:2014-09-29 Online:2015-04-07 Published:2015-04-07

摘要/Abstract

摘要： 为了剖析‘成恢177’稻瘟病持久抗性和高配合力特性的遗传基础，利用稻瘟病抗谱测定、抗病基因测序和SSR分子标记多态性比较的方法，分析了‘成恢177’的稻瘟病抗性和遗传背景。结果表明：在测试的63个稻瘟病单胞菌株和16个鉴别菌株中，‘成恢177’的抗性频率分别为66.67%和68.75%；‘成恢177’的稻瘟病抗性基因来自Lemont，为Pi-km。分子遗传背景分析表明，在148个SSR多态性位点中，‘成恢177’具有70.95%的‘绵恢502’位点，20.95%为美国热带粳稻品种Lemont位点；且在第1~10染色体上SSR多态性位点属‘绵恢502’较多(64.00%~100.00%)，在第11、12染色体上属Lemont较多(77.78%、60.00%)。本研究认为‘成恢177’成功地聚合了Lemont的稻瘟病抗性和‘绵恢502’的高配合力。

关键词: 水稻, 水稻, 硅高效, 筛选, 经济系数, 吸收特征

Abstract: In order to explore durable blast resistance and genetic basis of high combining ability in ‘Chenghui 177’, the resistant spectrum, blast resistance gene, and genetic background were analyzed using in vitro inoculation, gene sequencing and the polymorphism of SSR molecular markers. The resistance frequencies of ‘Chenghui 177’ against 63 isolates of rice blast and 16 differential isolates were 66.67% and 68.75%, respectively. It carried Pikm derived from Lemont which was a tropical japonica variety. The results of molecular genetic background analysis showed that, among 148 polymorphic SSR loci, ‘Chenghui 177’ had acquired respectively 70.95% and 20.95% from ‘Mianhui 502’ and Lemont. In the genome of ‘Chenghui 177’, 64% to 100% of the polymorphic SSR loci on chromosome 1-10 were derived from ‘Mianhui 502’, while Lemont was responsible for respectively 77.78% and 60.00% of the polymorphic SSR loci on chromosome 11 and 12 in ‘Chenghui 177’. The results suggested that ‘Chenghui 177’ successfully pyramided stable blast resistance of Lemont and high combining ability of ‘Mianhui 502’.

任鄄胜,陆贤军,高方远,彭云良,吴贤婷,苏相文,罗正良,张雪梅,任光俊. 水稻恢复系‘成恢177’稻瘟病抗性与遗传背景分析[J]. 中国农学通报, 2015, 31(9): 31-39.

参考文献

[1] 姬红丽,廖华明,龚爱清,等.四川稻瘟病菌群体致病性变异新趋势[J].西南农业学报,2005,18(3):286-290.
[2] 冯代贵,彭国亮.水稻品种抗病性变化与稻瘟病菌致病性变异的相关效应研究[J].植物病理学报,1995,25(2):184.
[3] 潘学贤,程开禄,黄富,等.水稻品种抗稻瘟病性丧失规律[J].云南农业大学学报,2004,19(5):536-540.
[4] 吴俊,刘雄伦,戴良英,等.水稻广谱抗稻瘟病基因研究进展[J].生命科学,2007(2):233-238.
[5] 朱小源,杨祁云,刘斌,等.三黄占2号及其衍生品种抗稻瘟病特征研究[J].广东农业科学,2003(2):37-40.
[6] 雷捷成,游年顺,黄利兴,等.籼稻不育系福伊A选育与利用[J].杂交水稻,1998,13(3):8-11.
[7] 雷捷成,游年顺,黄利兴,等.地谷A主要特性及利用技术[J].杂交水稻,1992(4):32-35.
[8] 陆贤军,任光俊,李青茂,等.优质抗稻瘟病水稻恢复系成恢 177 的选育与利用[J].杂交水稻,2007,22(2):18-21.
[9] 陆贤军,任光俊.优质香型杂交水稻新组合川香优2号[J].杂交水稻,2002,17(6):56.
[10] 67, R.n., Registration of `Lemont' rice[EB/OL]. http://archive.gramene.org/newsletters/varieties/Lemont.html.
[11] 王玉平,李仕贵,黎汉云,等.高配合力优质水稻恢复系蜀恢527的选育与利用[J].杂交水稻,2004,19(4):12-14.
[12] 张雪梅,冯慧,白玉连,等.四川省杂交稻主栽品种抗稻瘟病基因型推导研究[J].西南农业学报,2012,25(2):894-897.
[13] Murray M, Thompson W F. Rapid isolation of high molecular weight plant DNA[J]. Nucleic acids research,1980,8(19):4321-4326.
[14] Ren J, Yu Y, Gao F, et al. Application of resistance gene analog markers to analyses of genetic structure and diversity in rice[J]. Genome,2013,56(7):377-387.
[15] Costanzo S, Jia Y. Sequence variation at the rice blast resistance gene Pi-km locus: Implications for the development of allele specific markers[J]. Plant Science,2010,178(6):523-530.
[16] Wang Z, Jia Y, Rutger J, et al. Rapid survey for presence of a blast resistance gene Pi-ta in rice cultivars using the dominant DNA markers derived from portions of the Pi-ta gene[J]. Plant breeding,2007,126(1):36-42.
[17] Ashikawa I, Hayashi N, Yamane H, et al. Two adjacent nucleotide-binding site-leucine-rich repeat class genes are required to confer Pikm-specific rice blast resistance[J]. Genetics,2008,180(4):2267-2276.
[18] 杨勤忠,林菲,冯淑杰,等.水稻稻瘟病抗性基因的分子定位及克隆研究进展[J].中国农业科学,2009,42(5):1601-1615.
[19] Chen H, Chen B, Zhang D, et al. Pathotypes of Pyricularia grisea in rice fields of central and southern China[J]. Plant Disease,2001,85(8):843-850.
[20] Liu G, Lu G, Zeng L, et al. Two broad-spectrum blast resistance genes, Pi9(t) and Pi2(t), are physically linked on rice chromosome 6[J]. Mol Genet Genomics,2002,267(4):472-480.
[21] Jeon J S, Chen D, Yi G H, et al. Genetic and physical mapping of Pi5(t), a locus associated with broad-spectrum resistance to rice blast[J]. Mol Genet Genomics,2003,269(2):280-289.
[22] Berruyer R, Adreit H, Milazzo J, et al. Identification and fine mapping of Pi33, the rice resistance gene corresponding to the Magnaporthe grisea avirulence gene ACE1[J]. Theor Appl Genet, 2003,107(6):1139-1147.
[23] Conaway-Bormans C A, Marchetti M A, Johnson C W, et al. Molecular markers linked to the blast resistance gene Pi-z in rice for use in marker-assisted selection[J]. Theor Appl Genet,2003,107(6):1014-1020.
[24] Qu S, Liu G, Zhou B, et al. The broad-spectrum blast resistance gene Pi9 encodes a nucleotide-binding site-leucine-rich repeat protein and is a member of a multigene family in rice[J]. Genetics,2006,172(3):1901-1914.
[25] Deng Y, Zhu X, Shen Y, et al. Genetic characterization and fine mapping of the blast resistance locus Pigm (t) tightly linked to Pi2 and Pi9 in a broad-spectrum resistant Chinese variety[J]. Theoretical and applied genetics,2006,113(4):705-713.
[26] Xu X, Hayashi N, Wang C, et al. Efficient authentic fine mapping of the rice blast resistance gene Pik-h in the Pik cluster, using new Pik-h-differentiating isolates[J]. Molecular breeding,2008,22(2):289-299.
[27] Suh J P, Roh J H, Cho Y C, et al. The pi40 gene for durable resistance to rice blast and molecular analysis of pi40-advanced backcross breeding lines[J]. Phytopathology,2009,99(3):243-250.
[28] 冯慧,杨成明,吴孝波,等.四川省杂交稻亲本及 32 份抗源抗稻瘟病基因的检测分析[J].西南农业学报,2013,26(3):987-993.
[29] Tabien R, Li Z, Paterson A, et al. Mapping of four major rice blast resistance genes from ’Lemont’ and ’Teqing’ and evaluation of their combinatorial effect for field resistance[J]. Theoretical and Applied Genetics,2000,101(8):1215-1225.
[30] 张雪梅,冯慧,白玉莲,等.水稻抗稻瘟病基因和杂交稻品种在四川的抗性评价[J].西南农业学报,2012,25(4):1266-1272.
[31] Zhou B, Qu S, Liu G, et al. The eight amino-acid differences within three leucine-rich repeats between Pi2 and Piz-t resistance proteins determine the resistance specificity to Magnaporthe grisea[J]. Molecular plant-microbe interactions,2006,19(11):1216-1228.
[32] Zhai C, Lin F, Dong Z, et al. The isolation and characterization of Pik, a rice blast resistance gene which emerged after rice domestication[J]. New Phytol,2011,189(1):321-334.
[33] 陈瑞,程在全,黄兴奇,等.水稻优良性状控制基因的定位进展及其在染色体上的分布[J].遗传,2007,29(4):399-412.
[34] 吴方喜,蔡秋华,朱永生,等.籼型杂交稻恢复系明恢63的利用与创新[J].福建农业学报,2011,26(6):1101-1112.
[35] Yu S, Li J, Xu C, et al. Importance of epistasis as the genetic basis of heterosis in an elite rice hybrid[J]. Proceedings of the National Academy of Sciences,1997,94(17):9226-9231.
[36] Liu T, Shao D, Kovi M R, et al. Mapping and validation of quantitative trait loci for spikelets per panicle and 1,000-grain weight in rice (Oryza sativa L.)[J]. Theoretical and applied genetics,2010,120(5):933-942.
[37] Xue W, Xing Y, Weng X, et al. Natural variation in Ghd7 is an important regulator of heading date and yield potential in rice[J]. Nature genetics,2008,40(6):761-767.
[38] Xing Y, Tan Y, Hua J, et al. Characterization of the main effects, epistatic effects and their environmental interactions of QTLs on the genetic basis of yield traits in rice[J]. Theoretical and Applied Genetics,2002,105(2-3):248-257.、
[39] Consortia, T R C a S. The sequence of rice chromosomes 11 and 12, rich in disease resistance genes and recent gene duplications[J]. BMC Biol,2005(3):20.
[40] 李平,朱立煌,陈英,等.水稻花药培养植株后代的DNA变异[J].遗传学报,1995,22(4):286-292.
[41] 张楷正,缪杰,李季航,等.水稻籼粳杂交BC2F5代株系花药培养H2代株系的主要变异性状分析[J].分子植物育种,2007,5(1):79-83.
[42] Müller E, Brown P, Hartke S, et al. DNA variation in tissue-culture-derived rice plants[J]. Theoretical and applied genetics,1990,80(5):673-679.

水稻恢复系‘成恢177’稻瘟病抗性与遗传背景分析

Analysis of Blast Resistance and Genetic Background in Rice Restorer ‘Chenghui 177’

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

关于本刊

作者中心

审者中心

在线期刊

联系我们

[1]	白玛仁增, 顿玉多吉, 德例归吉, 德吉央宗, 益西多吉, 边巴次仁. 星-地结合对水稻高温热害监测模型的研究[J]. 中国农学通报, 2023, 39(1): 133-141.
[2]	罗先富, 刘文强, 潘孝武, 董铮, 刘三雄, 刘利成, 阳标仁, 盛新年, 李小湘. 应用剩余杂合体衍生的近等基因系定位水稻株高QTL[J]. 中国农学通报, 2022, 38(9): 1-5.
[3]	黄钰, 陈斌, 肖关丽. 云南哈尼族地方水稻‘月亮谷’对褐飞虱取食危害的生理反应[J]. 中国农学通报, 2022, 38(9): 123-129.
[4]	李兴华, 王欢, 张盛, 蔡星星, 周强, 周楠. 氮肥用量与运筹方式对晚籼稻产量及花后干物质积累与转运的影响[J]. 中国农学通报, 2022, 38(9): 6-13.
[5]	王一凡, 劳晓璨, 余丽萍, 叶海龙. 水稻‘甬优15’分期播种的气象条件适宜性试验研究[J]. 中国农学通报, 2022, 38(7): 106-109.
[6]	李雪枫, 王坚, 叶晓园, 张秀婷, 王丽学. 苦瓜植株水浸提液对水稻种子萌发和秧苗生长的影响[J]. 中国农学通报, 2022, 38(6): 1-7.
[7]	闫蕴韬, 何兮, 张海清, 贺记外. 水稻种子耐贮性研究进展[J]. 中国农学通报, 2022, 38(5): 1-8.
[8]	翟彩娇, 张蛟, 崔士友, 陈澎军. 盐逆境对耐盐水稻穗部性状及产量构成因素的影响[J]. 中国农学通报, 2022, 38(4): 1-9.
[9]	李荣田, 时柳, 黄丽莹, 刘长华. 利用分子选择培育水稻‘吉粳88’(hd2/hd4)导入系[J]. 中国农学通报, 2022, 38(33): 1-9.
[10]	伊嘉雯, 冯棣, 朱崴, 亓娜, 滕奉魁, 卢小引. 不同品种水稻发芽阶段耐盐性对比研究[J]. 中国农学通报, 2022, 38(33): 10-14.
[11]	努尔夏提·努尔买买提, 李焕宇, 杨成德. 辣椒根腐病菌拮抗链霉菌的筛选及16S rRNA基因系统发育分析[J]. 中国农学通报, 2022, 38(33): 116-123.
[12]	张博, 石峰, 宋福强. AMF复合菌剂对寒地水稻光合作用和生长效应的影响[J]. 中国农学通报, 2022, 38(33): 15-22.
[13]	许丹阳, 李虹颖, 孙义祥, 邬刚, 王家宝, 袁嫚嫚, 王佩旋, 张祥明, 束孝海. 不同比例有机无机肥配施对水稻产量与氮素利用率的影响[J]. 中国农学通报, 2022, 38(31): 1-5.
[14]	番华彩, 曾莉, 李卫雁, 丁云秀, 郭志祥, 李舒, 徐胜涛, 郑泗军, 王永斌. 香蕉细菌性软腐病防控药剂筛选及田间应用效果评价[J]. 中国农学通报, 2022, 38(31): 113-118.
[15]	王洋, 张瑞, 周雨晴, 刘永昊, 刘高生, 戴其根. 基于文献计量的国内水稻耐盐性研究态势分析[J]. 中国农学通报, 2022, 38(31): 147-153.