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中国农学通报 ›› 2016, Vol. 32 ›› Issue (5): 10-15.doi: 10.11924/j.issn.1000-6850.casb15090065

所属专题: 农业生态

• 水产 渔业 • 上一篇    下一篇

高通量测序技术在人工湿地微生物多样性研究中的研究进展

王林,李冰,朱健   

  1. 中国水产科学研究院淡水渔业研究中心遗传育种室,中国水产科学研究院淡水渔业研究中心遗传育种室,南京农业大学无锡渔业学院
  • 收稿日期:2015-09-16 修回日期:2015-10-19 接受日期:2015-10-22 出版日期:2016-02-19 发布日期:2016-02-19
  • 通讯作者: 朱健
  • 基金资助:
    中央级公益性科研院所基本科研业务费专项“复合湿地植物根系与基质微生物及浮游动植物群落动态结构的研究”(2015JBFM24);“十二·五”国家科技支撑计划“长江下游池塘高效生态养殖技术集成与示范”(2012BAD25B07);国家大宗淡水鱼类产业技术体系项目“国家大宗淡水鱼类产业技术体系”(nyeytx-46)。

Review of High-throughput Sequencing Techniques in Studies of constructedwetland Microbial Diversity

  • Received:2015-09-16 Revised:2015-10-19 Accepted:2015-10-22 Online:2016-02-19 Published:2016-02-19

摘要: 为了能够从微生物多样性的角度解释湿地净化水体的原理。本文分析了下一代代测序技术——高通量测序技术的过程和原理,总结了高通量测序技术应用的优缺点和高通量测序技术在人工湿地微生物多样性中的应用。同时本文得出了高通量测序技术在人工湿地微生物群落的多样性和动态性中具有很大的应用价值,指出了高通量测序结果中处理庞大数据等问题。但是高通量测序技术具有其独特的优点,对人工湿地微生物多样性的研究具有很大的应用前景。

关键词: 土地整治, 土地整治, 社会影响评价, 系统动力学, 模糊综合评价

Abstract: The paper aims to explain the theory of purifying the water body with wetland from the perspective of microbial diversity. The process and principle of the next- generation sequencing technology—highthroughput sequencing technology was analyzed, the advantages and disadvantages of high- throughput sequencing technology applications and the high-throughput sequencing technology application in wetland microbial diversity were summarized in this paper. Meanwhile the broad application value of high-throughput sequencing technology in the diversity and dynamics of the microbial community of artificial wetland was obtained, the high-throughput sequencing results in huge data processing and other issues were pointed out in this paper. Because the high- throughput sequencing technology has unique advantages, it has a great application prospect in the study of wetland microbial diversity.

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