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中国农学通报 ›› 2016, Vol. 32 ›› Issue (28): 70-76.doi: 10.11924/j.issn.1000-6850.casb16020059

所属专题: 生物技术 园艺

• 林学 园艺 园林 • 上一篇    下一篇

SA浸种对NaCl胁迫下西葫芦种子α-淀粉酶活性的影响

周正霄   

  1. (山西省临汾市尧都区气象局,山西临汾 041000)
  • 收稿日期:2016-02-19 修回日期:2016-09-20 接受日期:2016-04-25 出版日期:2016-10-11 发布日期:2016-10-11
  • 通讯作者: 周正霄
  • 基金资助:
    无基金。页数:10。图(片)数:8。表格数:4。

Effect of SA Soaking on α-amylase Activity of Marrow Squash Seeds under NaCl Stress

Zhou Zhengxiao   

  1. (Yaodu District Meteorological Bureau of Linfen City, Shanxi Province, Linfen Shanxi 041000)
  • Received:2016-02-19 Revised:2016-09-20 Accepted:2016-04-25 Online:2016-10-11 Published:2016-10-11

摘要: 研究盐胁迫下不同SA浸种条件对西葫芦种子α-淀粉酶活性的影响。研究盐胁迫下不同浓度、温度、时间的SA浸种,并利用响应面软件对浸种条件优化,分析对α-淀粉酶活性的影响。结果表明:浸种温度对α-淀粉酶活性的影响最强,浸种时间对α-淀粉酶活性的影响最弱。单因素实验的分析中种子萌发最适的SA浓度为1.0 mmol/L,浸种的温度为30℃,浸种的时间为18 h。响应面法优化α-淀粉酶活性最大时的参数是:浸种SA浓度1.09 mmol/L,浸种温度28.26℃,浸种时间18.94 h,在此条件下,α-淀粉酶活性的理论值为0.264455 mg/(g·FW·min),与理论值的贴近度为97.98%。在最优浸种条件下α-淀粉酶活性最高,表明在此浸种条件盐胁迫下的西葫芦种子萌发力最好。

关键词: 冻结气温, 冻结气温, 潜水入流, 土壤质地, 砂壤土, 粉质粘壤土

Abstract: The research aimed to study the effects of salicylic acid (SA) soaking on α-amylase activity of marrow squash seeds under salt stress. SA soaking of different concentrations, temperatures and time was studied under salt stress, and the soaking conditions were optimized with response surface software. SA soaking effects on α-amylase activity were analyzed. The results showed that soaking temperature had the strongest effect on α-amylase activity, while soaking time had the weakest effect. The optimal concentration of SA was 1.0 mmol/L, the optimal soaking temperature was 30℃, and the optimal soaking time was 18 h in single factor test. The maximum parameter of α-amylase activity optimized by response surface methodology was SA concentration of 1.09 mmol/L, soaking temperature of 28.26℃ and soaking time of 18.94 h. Under this condition, the value of α-amylase activity was 0.264455 mg/(g·FW·min) with 97.98%closeness of the theoretical value. The value of α-amylase activity was the maximum in the optimal soaking condition, indicating that the seed germination of marrow squash in this soaking condition was the best.