灵昆鸡VLDLR 和CTSD 基因多态性与产蛋性状的相关性研究

doi:10.11924/j.issn.1000-6850.casb16030010

中国农学通报 ›› 2016, Vol. 32 ›› Issue (26): 6-14.doi: 10.11924/j.issn.1000-6850.casb16030010

所属专题：生物技术；畜牧兽医

灵昆鸡VLDLR 和CTSD 基因多态性与产蛋性状的相关性研究

张丽萍^1,2,刘雅丽³,熊化鑫^1,2,曾涛¹,田勇¹,周树和⁴,林兴钦⁴,卢立志¹

(¹浙江省农业科学院畜牧兽医所,杭州 310021；²浙江师范大学化学与生命科学学院,浙江金华 321004；³浙江省畜牧技术推广总站,杭州 310029；⁴温州市金土地农业开发有限公司,浙江温州 325000）

收稿日期:2016-03-01 修回日期:2016-04-18 接受日期:2016-04-25 出版日期:2016-09-21 发布日期:2016-09-21
通讯作者: 张丽萍
基金资助:
浙江省重大科技专项重大农业项目“肉鸡全产业链质量安全控制与溯源技术体系研究与应用示范”(2014C02001)；浙江省农业科技成果转化项目“浙江省特色家禽遗传资源——灵昆鸡示范推广”。

Correlation of Single Nucleotide Polymorphisms of VLDLR and CTSD Geneswith Egg Production Traits in Lingkun Chicken

Zhang Liping^1,2, Liu Yali³, Xiong Huaxin^1,2, Zeng Tao¹, Tian Yong¹, Zhou Shuhe⁴, Lin Xingqin⁴, Lu Lizhi¹

(¹Institute of Animal Husbandry and Veterinary Medicine, Zhejiang Academy of Agricultural Science, Hangzhou 310021;²Chemistry and Life Science College, Zhejiang Normal University, Jinhua Zhejiang 321004;³Zhejiang Animal Husbandry Techniques Extension Station, Hangzhou 310029;⁴Wenzhou Golden Land Agricultural Development Co., Ltd, Wenzhou Zhejiang 325000)

Received:2016-03-01 Revised:2016-04-18 Accepted:2016-04-25 Online:2016-09-21 Published:2016-09-21

摘要/Abstract

摘要： 为了研究极低密度脂蛋白受体(VLDLR)和溶酶体组织蛋白酶(CTSD)的单核苷酸多态性(SNPs)，寻找与灵昆鸡产蛋性状相关的候选分子标记，以276 只灵昆鸡母鸡为研究对象，采用直接测序法，检测VLDLR 和CTSD 基因在灵昆鸡中的SNPs，并分析各多态位点不同基因型与11 个产蛋性状的相关性。结果表明：VLDLR 基因存在1 个突变位点C13444T，该位点不同基因型与哈氏单位、蛋黄重及蛋黄比例呈显著相关(P<0.05)，在哈氏单位性状中，ZTW型为优势基因型，在蛋黄重及蛋黄比例性状中，ZCW型为优势基因型；CTSD 基因有5 个突变位点，其中，T2614C 对蛋白高度和哈氏单位有显著影响(P<0.05)，C9445T、C9475T 对500 日龄产蛋量和蛋重显著相关(P<0.05)，C9457T 对蛋重有显著影响(P<0.05)，T11028C位点对蛋白高度有显著影响(P<0.05)。C13444T、C9457T、T11028C位点偏离Hardy-Weinberg平衡状态(P<0.05)，其他9 个位点均处Hardy-Weinberg 平衡状态(P>0.05)。结果提示：VLDLR 和CTSD基因多态性可能与产蛋性状有关，可尝试作为灵昆鸡产蛋性状分子标记的候选基因，为以后的选育工作提供理论依据。

关键词: 砧木, 砧木, 嫁接, 西瓜, 抗病性, 丰产性

Abstract: In order to study the single nucleotide polymorphisms (SNPs) of very low density lipoprotein receptor (VLDLR) and cathepsin D (CTSD), and find related candidate molecular markers of egg production traits in Lingkun chicken, SNPs of VLDLR and CTSD genes of 276 hens were screened by direct DNA sequencing and its correlation with egg production traits was analyzed to detect the molecular marker of the traits. The results showed that there was a SNP C13444T in VLDLR gene. The mutation site of C13444T was significantly correlated with Haugh unit (P<0.05) with Z^TW being the advantage genotype; and it also was significantly correlated with yolk weight and yolk proportion (P<0.05) with Z^CW being the advantage genotype. There were 5 SNPs (T2614C, C9445T, C9457T, C9475T, T11028C) in the exon 3, 7, 9 of CTSD gene. The mutation sites of T2614C was significantly correlated with Haugh unit and albumen height (P<0.05); the mutation sites of C9445T, C9475T were significantly correlated with 500-day-old egg production and egg weight, and the mutation site of C9457T was significantly correlated with egg weight; the mutation site of T11028C was significantly correlated with albumen height. The 3 SNPs (C13444T, C9457T, T11028C) were not in the Hardy-Weinberg equilibrium, the others were in Hardy-Weinberg equilibrium. The results indicated that egg production traits were correlated with VLDLR and CTSD genes which could be the candidate molecular markers, and provided the basic data for the breeding of Lingkun chicken.

张丽萍,刘雅丽,熊化鑫,曾涛,田勇,周树和,林兴钦,卢立志. 灵昆鸡VLDLR 和CTSD 基因多态性与产蛋性状的相关性研究[J]. 中国农学通报, 2016, 32(26): 6-14.

Zhang Liping,Liu Yali,Xiong Huaxin,Zeng Tao,Tian Yong,Zhou Shuhe,Lin Xingqin and Lu Lizhi. Correlation of Single Nucleotide Polymorphisms of VLDLR and CTSD Geneswith Egg Production Traits in Lingkun Chicken[J]. Chinese Agricultural Science Bulletin, 2016, 32(26): 6-14.

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灵昆鸡VLDLR 和CTSD 基因多态性与产蛋性状的相关性研究

Correlation of Single Nucleotide Polymorphisms of VLDLR and CTSD Geneswith Egg Production Traits in Lingkun Chicken

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