黄瓜NRT3 基因的鉴定和特征分析

doi:10.11924/j.issn.1000-6850.casb16030225

中国农学通报 ›› 2016, Vol. 32 ›› Issue (22): 117-121.doi: 10.11924/j.issn.1000-6850.casb16030225

所属专题：生物技术；园艺

黄瓜NRT3 基因的鉴定和特征分析

孙建磊,董玉梅,王崇启,肖守华,王晓,李利斌,焦自高

(山东省农业科学院蔬菜花卉研究所/山东省设施蔬菜生物学重点实验室/国家蔬菜改良中心山东分中心,济南 250100）

收稿日期:2016-03-31 修回日期:2016-07-18 接受日期:2016-05-25 出版日期:2016-08-09 发布日期:2016-08-09
通讯作者: 孙建磊
基金资助:
山东省农业科学院青年科研基金项目“低温胁迫下黄瓜NRT家族基因的表达调控研究”(2015YQN29)；山东省农业良种工程项目“设施蔬菜新品种选育”(2014-03-07)。

Identification and Characterization of NRT3 Genes from Cucumber

Sun Jianlei, Dong Yumei, Wang Chongqi, Xiao Shouhua, Wang Xiao, Li Libin, Jiao Zigao

(Institute of Vegetables and Flowers, Shandong Academy of Agricultural Sciences/Shandong Key Laboratory of Greenhouse Vegetable Biology/Shandong Branch of National Vegetable Improvement Center, Jinan 250100)

Received:2016-03-31 Revised:2016-07-18 Accepted:2016-05-25 Online:2016-08-09 Published:2016-08-09

摘要/Abstract

摘要： 旨在探讨黄瓜NRT3基因的遗传进化和功能，为进一步研究黄瓜NRT3基因的功能提供依据。利用生物信息学方法，从黄瓜基因组中鉴定出2个NRT3基因，并对这些基因的基因组分布、结构、遗传进化和顺式元件进行了系统分析。结果显示，黄瓜的NRT3两个基因均位于1号染色体，具有1个或者2个外显子，分别与拟南芥的NRT3.1或者NRT3.2直系同源。NRT3在瓜类作物间编码蛋白的序列非常保守，它们都具有数个植物激素和逆境应答元件，而且黄瓜和甜瓜的直系同源NRT3基因的顺式调控元件存在较大差异。本研究为进一步研究黄瓜NRT3的遗传进化和功能提供了线索。

关键词: 水稻土, 水稻土, “3414”试验, 磷, 钾, 养分丰缺指标

Abstract: The aims were to explore the genetic evolution and function of cucumber NRT3 genes, and provide evidence for further studies on cucumber NRT3 gene. Two cucumber NRT3 genes were identified by bioinformatics method, and their genome distribution, gene structure, phylogeny, and cis-elements were systematically analyzed. The results showed that, two cucumber NRT3 genes were both located in chromosome 1, and they had one or two exons, they were orthologous to Arabidopsis NRT3.1 or NRT3.2 respectively. The putative NRT3 protein sequences of cucurbit crops were conserved extremely. And also they had several putative cis-elements responsive to plant hormones and environmental stresses. The cis-elements of cucumber and melon orthologous NRT3 genes had great difference. This study provided a clue for investigating the function and evolution of the cucumber NRT3 genes.

孙建磊,董玉梅,王崇启,肖守华,王晓,李利斌,焦自高. 黄瓜NRT3 基因的鉴定和特征分析[J]. 中国农学通报, 2016, 32(22): 117-121.

Sun Jianlei,Dong Yumei,Wang Chongqi,Xiao Shouhua,Wang Xiao,Li Libin and Jiao Zigao. Identification and Characterization of NRT3 Genes from Cucumber[J]. Chinese Agricultural Science Bulletin, 2016, 32(22): 117-121.

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黄瓜NRT3 基因的鉴定和特征分析

Identification and Characterization of NRT3 Genes from Cucumber

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