白羊草BiCDPK基因保守区的克隆及序列分析

doi:10.11924/j.issn.1000-6850.casb16040171

中国农学通报 ›› 2016, Vol. 32 ›› Issue (24): 130-135.doi: 10.11924/j.issn.1000-6850.casb16040171

所属专题：生物技术；畜牧兽医

白羊草BiCDPK基因保守区的克隆及序列分析

王运琦¹,方志红¹,任彬琳¹,李莲芬¹,董宽虎²

(¹山西省农业科学院畜牧兽医研究所,太原 030032；²山西农业大学动物科技学院,山西太谷 030801）

收稿日期:2016-04-27 修回日期:2016-08-04 接受日期:2016-06-24 出版日期:2016-08-29 发布日期:2016-08-29
通讯作者: 董宽虎
基金资助:
教育部高等学校博士学科点专项科研基金项目“白羊草抗逆转录因子ERF的克隆与功能鉴定”(20101403110002)。

The Clone and Sequence Analysis of BiCDPK Conserved Sequence from Bothriochloa ischaemum

Wang Yunqi¹, Fang Zhihong¹, Ren Binlin¹, Li Lianfen¹, Dong Kuanhu²

(¹Animal Husbandry and Veterinary Institute, Shanxi Academy of Agricultural Sciences, Taiyuan 030032;²College of Animal Science and Technology, Shanxi Agricultural University, Taigu Shanxi 030801)

Received:2016-04-27 Revised:2016-08-04 Accepted:2016-06-24 Online:2016-08-29 Published:2016-08-29

摘要/Abstract

摘要： 为了研究CDPK基因的结构和功能，利用RT-PCR方法从白羊草中克隆得到BiCDPK基因，并对其进行生物信息学分析。结果表明：该BiCDPK基因cDNA长1048 bp，包含387 bp的开放阅读框，编码128个氨基酸。蛋白表现为弱酸性，且稳定、亲水，二级结构中以无规则卷曲和α-螺旋为主。亚细胞定位于细胞质中，无跨膜结构域，无信号肽。蛋白序列中存在1个丝氨酸磷酸化位点和3个苏氨酸磷酸化位点。进化分析结果表明，克隆的BiCDPK基因与玉米、小麦和水稻具有较高同源性，相似度分别是97%、86%、86%。为进一步研究BiCDPK基因的功能奠定了基础。

关键词: 天水市, 天水市, 气候变化, 热量资源, 农业生产, 影响

Abstract: In order to study the structure and function of CDPK gene, BiCDPK was cloned from Bothriochloa ischaemum by using RT-PCR method, and analyzed by bioinformatics. The results showed that the length of cDNA for BiCDPK was 1048 bp, which contained a 387 bp complete open reading frame encoding 128 amino acids. The corresponding protein was mildly acidic, stable and hydrophilic, with random coil and α-helix as main components in secondary structure. Subcellular localization indicated that the protein was located in cytoplasmic without transmembrane region and signal peptide. The sequence of protein included one serine phosphorylation sites and three threonine phosphorylation sites. Further phylogenic analysis displayed that BiCDPK in Zea mays, Triticum aestivum and Oryza sativa shared 97%, 86% and 86% identity. It laid a foundation to further study the BiCDPK function in Bothriochloa ischaemum.

王运琦,方志红,任彬琳,李莲芬,董宽虎. 白羊草BiCDPK基因保守区的克隆及序列分析[J]. 中国农学通报, 2016, 32(24): 130-135.

Wang Yunqi,Fang Zhihong,Ren Binlin,Li Lianfen and Dong Kuanhu. The Clone and Sequence Analysis of BiCDPK Conserved Sequence from Bothriochloa ischaemum[J]. Chinese Agricultural Science Bulletin, 2016, 32(24): 130-135.

参考文献

[1] 康乐.烟草钙依赖蛋白激酶基因克隆与分析[D].北京:北京农业科学院,2013.
[2] Asano T, Kunieda N, Omura Y, et al. Rice SPK, a calmodulin-like domain protein kinase, is required for storage product accumulation during seed development: phosphorylation of sucrose synthase is a possible factor[J]. Plant Cell, 2002,14(3):619-628.
[3] Saijo Y, Hata S, Kyozuka J, et al. Over-expression of a single Ca²⁺-dependent protein kinase confers both cold and salt/drought tolerance on rice plants[J]. Plant J, 2000,23(3):319-327.
[4] Romeis T, Ludwig A A, Martin R, et al. Calcium-dependent protein kinases play an essential role in a plant defence response[J]. EMBO J, 2001,20(20):5556-5567.
[5] Romeis T, Piedras P, Jones J D. Resistance gene-dependent activation of a calcium-dependent protein kinase in the plant defense response[J]. Plant Cell, 2000, 12(5): 803-816.
[6] Ivashuta S, Liu J, Lohar D P, et al. RNA interference identifies a calcium-dependent protein kinase involved in Medicago truncatula root development[J]. Plant Cell, 2005,17(11):2911-2921.
[7] Gargantini P R, Gonzalez-Rizzo S, Chinchilla D, et al. A CDPK isoform participates in the regulation of nodule number in Medicago truncatula[J]. Plant J, 2006,48(6):843-856.
[8] Choi H I, Park H J, et al. Arabidopsis calcium-dependent protein kinase AtCPK32 interacts with ABF4, a transcriptional regulator of abscisic acid-responsive gene expression, and modulates its activity[J]. Plant Physiol, 2005,139(4):1750-1761.
[9] Yoon G M, Dowd P E, Gilroy S, et al. Calcium-dependent protein kinase isoforms in petunia have distinct functions in pollen tube growth, including regulating polarity[J]. Plant Cell, 2006,18(4):867-878.
[10] 倪天华,魏幼璋.钙依赖型蛋白激酶(CDPKs)在植物中的生理功能[J].西北农林科技大学学报,2002,30:241-246.
[11] Krupa A, Anamika, Srinivasan N. Genome-wide comparative analyses of domain organisation of repertoires of protein kinases of Arabidopsis thaliana and Oryza sativa[J]. Gene, 2006,380(1):1-13.
[12] 刘贯山,陈珈.钙依赖蛋白激酶(CDPKs)在植物钙信号转导中的作用[J].植物学通报,2003,20(2):160-167.
[13] Mori I C, Murata Y, Yang Y Z, et al. CDPKs CPK6 and CPK3 function in ABA regulation of guard cell S-type anion- and Ca2+-permeable channels and stomatal closure[J]. PloS Biology, 2006,4:749-1762.
[14] Cheng S H, Willmann M R, Chen H C, et al. Calcium signaling through protein kinase. The Arabidopsis calcium-dependent protein kinase gene family[J]. Plant Physiology, 2002,129:469-485.
[15] Hrabak E M, Chan C W, Gribskov M, et al. The Arabidopsis CDPK-SnRK superfamily of protein kinases[J]. Plant Physiol, 2003,132(2):666-680.
[16] Asano T, Tanaka N, Yang G, et al. Genome-wide identification of the rice calcium-dependent protein kinase and its closely related kinase gene families: comprehensive analysis of the CDPKs gene family in rede[J]. Plant Cell Physiol, 2005,46(2):356-366.
[17] 张丽,张磊,康乐,等.绒毛状烟草钙依赖蛋白激酶基因家族分析[J].中国烟草科学,2012(3):56-62.
[18] Estelle M, Hrabak. The Arabidopsis CDPK-SnRK superfamily of protein kinase[J]. Plant Physiology, 2003,132(2):666-680.
[19] 陈硕,陈珈.植物中钙依赖蛋白激酶(CDPKs)的结构与功能[J].植物学通报,2001,18(2):143-148.
[20] 薛彬,夏新莉,尹伟伦.杨树钙依赖蛋白激酶基因家族生物信息学分析[J].经济林研究,2010,28(1):20-25.

白羊草BiCDPK基因保守区的克隆及序列分析

The Clone and Sequence Analysis of BiCDPK Conserved Sequence from Bothriochloa ischaemum

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