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中国农学通报

中国农学通报 ›› 2017, Vol. 33 ›› Issue (17): 82-86.doi: 10.11924/j.issn.1000-6850.casb16080061

• 资源 环境 生态 土壤 气象 • 上一篇    下一篇

海南近海石油降解菌的筛选、鉴定及降解性能

文少白,牛宗亮,季玉祥,唐天乐   

  1. (海南医学院热带医学与检验医学院,海口 571199）
  • 收稿日期:2016-08-15 修回日期:2016-12-20 接受日期:2016-12-23 出版日期:2017-06-16 发布日期:2017-06-16
  • 通讯作者: 唐天乐
  • 基金资助:
    海南省自然科学基金“海南近海石油类污染降解菌群的构建及降解性能研究”(20164165)；海南医学院科研培养基金“海南近海高效石油降解菌的筛选及其降解效果研究”(HY2013-13)、“多菌灵降解菌的筛选、表征及其在污染环境治理中的应用”(HY2013-16)。

Offshore Petroleum Degrading Bacteria: Screening, Identification and Degradation Property in Hainan

Wen Shaobai, Niu Zongliang, Ji Yuxiang, Tang Tianle   

  1. (Institute of Tropical Medicine and Inspection Division, Hainan Medical University, Haikou 571199)
  • Received:2016-08-15 Revised:2016-12-20 Accepted:2016-12-23 Online:2017-06-16 Published:2017-06-16

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摘要： 为筛选出适应海南海洋环境的高效石油降解菌，以柴油为唯一碳源，通过分离、初筛和复筛，从石油长期污染的海南近海海水中分离出1株高效的石油降解菌株X10，经16S rDNA序列分析，该菌株被鉴定为无色菌，其对柴油的降解率为69.39%。同时，研究分析不同pH值、温度、初始接种量、外加碳源对菌株柴油降解能力的影响。结果表明，在试验条件下，菌株X10在初始pH 6.0时降解效果最好，且该菌pH值适应范围较广，偏酸或偏碱的环境下该菌对柴油的降解效果均较好；培养温度对菌株石油降解率的影响较大，最佳温度为30℃，降解率达67.94%；最佳接种量为5%；添加外加碳源葡萄糖、乳糖、甘露醇对柴油降解率均有提高，但3种外加碳源间差异不显著。

关键词: 半矮化突变体, 半矮化突变体, 遗传分析, 基因定位, SSR, 水稻

Abstract: This study focused on the screening of highly efficient petroleum degrading bacteria. The petroleum-degrading strain X10 is based on diesel as the sole carbon source, which was separated by primary and secondary screening from the petroleum-contaminated coastal area of Hainan Province. 16S rDNA sequencing was showed that the strain was identified as Achromatium. Diesel was degraded is 69.39%. Meanwhile, the effects of pH, culture temperature, initial inoculation amount and external carbon source on the degradation ability of diesel were studied. The results showed that strain X10 had the best degradation effect at the initial pH 6.0 under the experimental conditions. The strain can adapt to a wide range of PH value. The culture temperature had a significant influence on the petroleum degradation rate. The degradation rate was 67.94% at 30℃, which is the optimum temperature. The optimum inoculation amount was 5%. The degradation rate of diesel was enhanced by the addition of external carbon sources, such as glucose, lactose and mannitol. However, there were no significant differences among these external carbon sources.