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中国农学通报

中国农学通报 ›› 2017, Vol. 33 ›› Issue (28): 99-103.doi: 10.11924/j.issn.1000-6850.casb16090098

所属专题： 生物技术

• 林学 园艺 园林 • 上一篇    下一篇

崇明水仙鳞片愈伤组织诱导和再分化影响因素研究

杨柳燕,蔡友铭,张昕欣,张永春   

  1. 上海市农业科学院林木果树研究所,上海市农业科学院林木果树研究所,河北省林业工程项目管理中心,上海市农业科学院林木果树研究所
  • 收稿日期:2016-09-21 修回日期:2017-09-25 接受日期:2017-01-05 出版日期:2017-10-24 发布日期:2017-10-24
  • 通讯作者: 张永春
  • 基金资助:
    上海市科委崇明专项子课题(13391912505)；上海市科委研发平台专项(15DZ2291000)；“上海市花卉产业技术体系建设”(沪农科产字（2017）第8号)；上海市科委技术中心建设专项(14DZ2251500)

Induction of Callus and Organogenesis from Scales in Chongming Daffodil

  • Received:2016-09-21 Revised:2017-09-25 Accepted:2017-01-05 Online:2017-10-24 Published:2017-10-24

摘要： 以崇明水仙鳞片为外植体材料，对愈伤组织的诱导和再分化过程中的影响因素进行研究，摸索出最有利的组培条件，获得崇明水仙试管小鳞茎，并对组培过程中冷处理时间、鳞片位置及激素配比等影响因子进行研究。结果表明：鳞茎冷处理时间以3 周为佳；内层鳞片诱导率和再分化率更高；冷处理3周的内层鳞片愈伤组织诱导和再分化的最佳培养基为MS+ BA 1 mg/L+ 2,4-D 0.1 mg/L+蔗糖30 g/L+琼脂4 g/L，诱导率和再分化率分别为48.4%和23.3%。试验摸索出了鳞片愈伤组织途径获得崇明水仙试管小鳞茎的最佳条件，为崇明水仙的规模化生产提供技术支持。

关键词: 碳足迹, 碳足迹, 肥料, 统计分析

Abstract: In this study, the scales were used as explants to induce callus and organogenesis of Chongming daffodil, in order to obtain the most favorable conditions of tissue culture. The influencing factors of induction of callus and organogenesis, such as low temperature treatment, the scales position and different combinations and concentrations of hormones were analyzed. The results showed that: the inner scales treated with low temperature (4℃) for 3 weeks was the suitable explants for inducing callus, the induction rate was 48.4%. The differentiation rate of callus was 23.3% on the MS medium with 1 mg/L BA, 0.1 mg/L 2,4-D, 30 g/L sucrose and 4 g/L agar. The experiment explored the optimal tissue culture condition to obtain the bulbsin vitro from the scales, and provided technical support for the large-scale production of Chongming daffodil.

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