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中国农学通报 ›› 2017, Vol. 33 ›› Issue (17): 24-31.doi: 10.11924/j.issn.1000-6850.casb17030137

所属专题: 生物技术 园艺

• 生物技术科学 • 上一篇    下一篇

辣椒GATA转录因子的生物信息学分析

袁 岐,张春利,赵婷婷,许向阳   

  1. (东北农业大学园艺园林学院,哈尔滨 150030)
  • 收稿日期:2017-03-21 修回日期:2017-05-09 接受日期:2017-04-21 出版日期:2017-06-16 发布日期:2017-06-16
  • 通讯作者: 袁岐
  • 基金资助:
    现代农业产业技术体系专项基金“国家大宗蔬菜产业技术体系岗位专家”(CARS-25-A-15);国家自然科学基金“抗叶霉病基因Cf19的克隆功能验证及防御信号传导途径的研究”(31272171);黑龙江省大宗蔬菜新品种选育“黑龙江省大宗蔬菜新品种选育”(GA15B103)。

Bioinformatics Analysis of GATA Transcription Factor in Pepper

Yuan Qi, Zhang Chunli, Zhao Tingting, Xu Xiangyang   

  1. (College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030)
  • Received:2017-03-21 Revised:2017-05-09 Accepted:2017-04-21 Online:2017-06-16 Published:2017-06-16

摘要: 本研究旨在分析和鉴定辣椒GATA转录因子的家族成员的生物信息学特征,为今后进一步的实验研究奠定基础。利用生物信息学手段,通过HMMER和SMART软件对辣椒GATA转录因子进行鉴定。利用Clustal X 1.83、MEGA5.05、GSDS、MEME等软件对辣椒GATA转录因子进行生物信息学分析。本研究鉴定出24个辣椒GATA转录因子家族成员。系统进化树的结果显示,24个GATA家族成员分为4个亚族。GSDS软件结果表明辣椒GATA转录因子的内含子、外显子分布情况。DNAMAN软件和MEME软件结果显示辣椒的大部分GATA转录因子是保守的。染色体定位软件MapInspect分析表明,辣椒GATA转录因子在辣椒12条染色体上分布并不均匀。本研究鉴定并分析了辣椒GATA转录因子的生物信息学的特征,为以后继续研究其功能提供了一定的理论基础与现实意义。

关键词: 无乳链球菌, 无乳链球菌, cfb基因, PCR, 地高辛标记探针, 原位杂交

Abstract: Abstract: The aim of this study was to analyze and identify the bioinformatics features of the family members of GATA transcription factors in pepper. It lays the foundation for further experimental research for the future.By means of bioinformatics means, HMMER and SMART software were used to identify the GATA transcription factors in pepper. Bioinformatics analysis of GATA transcription factor in pepper was carried out by software such as Clustal X 1.83, MEGA5.05, GSDS and MEME. This study identified 24 members of the GATA transcription factor family in pepper. The results of phylogenetic tree showed that the 24 GATA family members could be divided into 4 subgroups. The results of GSDS software showed that the distribution of intron and exon of GATA transcription factor in pepper. The results of DNAMAN software and MEME software showed that most of the GATA transcription factors in pepper were conserved. Chromosome localization software MapInspect analysis showed that the GATA transcription factor of pepper was not evenly distributed on 12 chromosomes of pepper. This study identified and analyzed the bioinformatics characteristics of GATA transcription factors in pepper, which provided a theoretical basis and practical significance for further study of its function.