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中国农学通报 ›› 2017, Vol. 33 ›› Issue (23): 10-15.doi: 10.11924/j.issn.1000-6850.casb17040042

所属专题: 园艺

• 生物技术科学 • 上一篇    下一篇

含喜树碱植物水培根系化感化合物的分离分析

林贤伟?,谭杰安?,刘展眉,程杏安,蒋旭红   

  1. 仲恺农业工程学院 天然产物化学研究所,仲恺农业工程学院 天然产物化学研究所,华南师范大学 化工与环境学院,仲恺农业工程学院 天然产物化学研究所,仲恺农业工程学院 天然产物化学研究所,仲恺农业工程学院 天然产物化学研究所
  • 收稿日期:2017-04-09 修回日期:2017-06-11 接受日期:2017-06-23 出版日期:2017-08-21 发布日期:2017-08-21
  • 通讯作者: 程杏安
  • 基金资助:
    国家自然科学基金项目“在线富集生物反应器培育特异性花生毛状根合成白藜芦醇”(201376281),“新型苦参碱衍生物的分子优化合成、 杀虫活性构效关系及机理研究”(21406274);广东省大学生创新训练项目“花生壳白藜芦醇提取工艺优化及种间含量分析”(201411347034),“几种天 然产物对黑色素瘤细胞增殖及其黑色素合成机制的影响”(201511347056)。

Analysis and Separation of Allelopathic Compounds from Hydroponic Root of Plants with Camptothecin

  • Received:2017-04-09 Revised:2017-06-11 Accepted:2017-06-23 Online:2017-08-21 Published:2017-08-21

摘要: 本研究旨在探明喜树碱植物水培体系中化感物质的种类及其长期积累对植物生产的作用。建立了喜树幼苗和野生型广州蛇根草水培体系根系分泌物的新分离富集方法:首先以硅胶填充固相萃取柱萃取水培液根系分泌物,然后取出填充硅胶加到硅胶层析柱上层进行柱层析分离,再经洗脱后,不同组分的洗脱液利用GC-MS技术进行分析,该方法实现了2种植物水培根系分泌物中化感物质的大量检出。结果从喜树水培根系分泌物中检测到1,1,2,2-四氯乙烷、N,N-二甲基苯胺、二苯铬等11种化感物质;在广州蛇根草水培根系分泌物中检测到邻苯二甲酸二丁酯、苯硒酚、二苯铬等14种化感物质。两种含喜树碱植物水培根系分泌物中化感物质的大量分离富集和分析,为清除水培体系的生长抑制物,优化水培体系,实现喜树碱的高效生物合成奠定基础。

关键词: 木醋液, 木醋液, 提取来源, 成分, 应用

Abstract: The purpose of this study is to identify the types of allelopathic compounds in a hydroponic system and their effects on plants if they accumulate in hydroponic root system of plant for a long time. A new technology of separating and enriching root secretions from acuminata seedlings and wild Ophiorrhiza cantoniensis cultivated in GUANGZHOU had been established: silica gel were used as a filler of the solid phase extraction column to extract compounds from the hydroponic solution; then took the filler out and added in the top of the Silica gel column to separate the allelopathic compounds, After elution, the technology of GC-MS were used to analyze the constituents. By this technology, many allelopathic compounds in hydroponic root secretions of these two plants had been identified. 11 allelopathic compounds were found in root secretion of acuminata seedlings such as Ethane, 1,1,2,2-tetrachloroethane, N,N-Dimethylaniline, dibenzene-chromium and so on.12 allelopathic compounds were identified in root secretion of Ophiorrhiza cantoniensis such as Dibutyl phthalate, Dioctyl phthalate, dibenzene-chromium and so on. Isolation, enrichment and analysis of allelochemicals in root exudates of two plant species containing camptothecin laid the foundation for eliminating growth inhibitors containing in root secretion of acuminata seedlings, optimizing water culture system and realizing high efficient biosynthesis of camptothecin.

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