雷蒙德氏棉SWEET 基因家族的全基因组鉴定和进化分析

doi:10.11924/j.issn.1000-6850.casb17040083

中国农学通报 ›› 2017, Vol. 33 ›› Issue (25): 13-21.doi: 10.11924/j.issn.1000-6850.casb17040083

所属专题：生物技术；棉花

雷蒙德氏棉SWEET 基因家族的全基因组鉴定和进化分析

陈慧敏,李威,马雄风,裴小雨,刘艳改,贺昆仑,张飞,张文生,王振玉,杨代刚,胡守林

塔里木大学植物科学学院,中国农业科学院棉花研究所,中国农业科学院棉花研究所,中国农业科学院棉花研究所,中国农业科学院棉花研究所,中国农业科学院棉花研究所,中国农业科学院棉花研究所,中国农业科学院棉花研究所,中国农业科学院棉花研究所,中国农业科学院棉花研究所,塔里木大学植物科学学院

收稿日期:2017-04-13 修回日期:2017-06-17 接受日期:2017-06-23 出版日期:2017-09-15 发布日期:2017-09-15
通讯作者: 胡守林
基金资助:
棉花生物学国家重点实验室基金“棉花产量相关SWEET 基因的功能分析”(CB2017C11)；国家棉花产业技术体系“中熟棉育种岗位” (CARS-18-05)。

Genome-wide Identification and Phylogenetic Analysis of SWEET Gene Family in Gossypium raimondii

Received:2017-04-13 Revised:2017-06-17 Accepted:2017-06-23 Online:2017-09-15 Published:2017-09-15

摘要/Abstract

摘要： 为了探索SWEET基因家族在棉花中的分布和作用，本文基于二倍体D基因组雷蒙德氏棉基因组数据，利用生物信息学方法对SWEET基因家族完成了全基因组水平的鉴定，并进行了系统发育、基因结构、染色体定位和跨膜结构域分析。共获得31个雷蒙德氏棉SWEET基因，分散或成簇分布在11条染色体上，分为三个不同功能的亚家族；跨膜结构域预测显示大部分雷蒙德氏棉SWEET基因含7个跨膜结构域，少部分含6或7个跨膜结构域；基因结构分析显示除GrSWEET6、GrSWEET7、GrSWEET9、GrSWEET19含4个内含子外，其余27个SWEET基因均含有5个内含子。这些结果将为进一步深入研究SWEET基因在棉花生长发育中功能奠定坚实的数据基础。

关键词: 缓/控释肥, 缓/控释肥, 水稻, 一次性施肥, 产量, 氮素农学利用率

Abstract: To study the distribution and function of SWEET gene family in cotton, based on the genome data of the diploid cottonGossypium raimondii (DD), the bioinformatics method was used to identify the whole genome level of SWEET gene family, and the phylogenetic, genetic structure, chromosome localization and transmembrane domain analysis were performed. A total of 31 Gossypium raimondii SWEET genes were obtained, distributed unevenly or clustered among 11 chromosomes, divided into three subfamilies of different functions. Transmembrane domain prediction showed that most of the GrSWEETs contained 7 transmembrane domains and a small fraction contained 6 or 8 transmembrane domains. Gene structure analysis showed that in addition to SWEET6, SWEET7, SWEET9 and SWEET19 with 4 introns, the remaining 27 SWEETs contained 5 introns. The results provide a solid data base for further study on functions of SWEET genes in cotton growth and development.

陈慧敏,李威,马雄风,裴小雨,刘艳改,贺昆仑,张飞,张文生,王振玉,杨代刚,胡守林. 雷蒙德氏棉SWEET 基因家族的全基因组鉴定和进化分析[J]. 中国农学通报, 2017, 33(25): 13-21.

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雷蒙德氏棉SWEET 基因家族的全基因组鉴定和进化分析

Genome-wide Identification and Phylogenetic Analysis of SWEET Gene Family in Gossypium raimondii

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