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中国农学通报 ›› 2018, Vol. 34 ›› Issue (12): 76-83.doi: 10.11924/j.issn.1000-6850.casb17120056

• 生物技术科学 • 上一篇    下一篇

基于 UPLC/Q-TOF-MS 代谢组学技术研究半枫荷根化学成分

田晓明,颜立红,蒋利媛,向光锋,何友军   

  1. 湖南省长沙市雨花区湖南省森林植物园410116,湖南省森林植物园,湖南省森林植物园,湖南省森林植物园,湖南省森林植物园
  • 收稿日期:2017-12-12 修回日期:2018-02-13 接受日期:2018-02-24 出版日期:2018-05-03 发布日期:2018-05-03
  • 通讯作者: 田晓明
  • 基金资助:
    湖南林业科技计划项目“半枫荷主要药用成分合成分子机制研究”(XLK201718)。

Chemical Constituents of Semiliquidambar cathayensis Chang Root Based on UPLC/Q-TOF/MS Metabonomics Technology

  • Received:2017-12-12 Revised:2018-02-13 Accepted:2018-02-24 Online:2018-05-03 Published:2018-05-03

摘要: 半枫荷为中国特有种,国家二级保护植物,其药用历史悠久,根、枝、叶、树皮和花蜜均可入药,具有祛风除湿,舒筋活血等功效。本研究对半枫荷根不同部位化学成分进行比较分析,以期为半枫荷根的合理应用和良种选育提供参考。通过超高效液相色谱-四级杆飞行时间质谱(UPLC/Q-TOF-MS)技术,从半枫荷根不同部位的色谱图中共鉴定了59种化合物,其中有机酸类16种、甾醇类化合物3种、天然多酚类物质5种、维生素4种、酰胺类化合物5种。采用主成分分析(PCA)等统计分析方法,找到了半枫荷根不同部位差异化学成分,发现共有差异成分主要有6种,包括瓜氨酸、胆酸、白藜芦醇、7-羰基豆甾醇、鞘氨醇和单半乳糖二酰甘油酯。其中瓜氨酸、胆酸、白藜芦醇和7-羰基豆甾醇等化学成分在根皮中含量最高。研究结果表明在半枫荷根不同部位化学成分存在一定差异且各具优势,这为今后半枫荷的定向选育和质量评价(即以抗炎镇痛活性物质为依据)提供了数据支持。

关键词: 水稻, 水稻, 电子辐照, 农艺性状, 生理生化指标

Abstract: In this study, the chemical constituents of different parts of Semiliquidambar cathayensis Chang root were compared and analyzed, to provide a reference for its rational application, selection and breeding. The chemical constituents of different parts ofSemiliquidambar cathayensis Chang root were analyzed by Ultra- High Performance Liquid Chromatography-Quadrupole Mass Spectrometry (UPLC-Q-TOF/MS). A total of 59 compounds were identified from the chromatograms of different parts of Semiliquidambar cathayensis Chang root. These compounds included 16 kinds of organic acids, 3 kinds of sterols compounds, 5 kinds of natural polyphenols, 4 kinds of vitamins and 5 kinds of amide compounds. Using principal component analysis (PCA) and other statistical analysis methods, we found that there were six common components in different parts of Semiliquidambar cathayensis Chang root, which were citrulline, cholic acid, resveratrol, 7- carbonyl stigmasterol, sphingosine alcohol and monoglucose diacylglyceride. The citrulline, cholic acid, resveratrol and 7-carbonyl stigmasterol and the other chemical components showed the highest values in the root bark. The study indicates that chemical constituents in different parts ofSemiliquidambar cathayensis Chang root have some differences and individual advantages, and provides data support for future directed breeding and quality evaluation of Semiliquidambar cathayensis Chang (based on antiphlogistic and analgesia active substances).