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中国农学通报 ›› 2018, Vol. 34 ›› Issue (20): 63-69.doi: 10.11924/j.issn.1000-6850.casb18020018

所属专题: 生物技术

• 生物技术科学 • 上一篇    下一篇

余甘子转录组分析

赵琼玲,)②金杰,) 罗会英,) 张永辉,) 何璐,) 瞿文林,) 沙毓沧   

  1. 云南省农业科学院热区生态农业研究所,云南省农业科学院热区生态农业研究所,云南省农业科学院热区生态农业研究所,云南省农业科学院热区生态农业研究所,云南省农业科学院热区生态农业研究所,云南省农业科学院热区生态农业研究所,云南省农业科学院热区生态农业研究所
  • 收稿日期:2018-02-03 修回日期:2018-04-04 接受日期:2018-04-24 出版日期:2018-07-13 发布日期:2018-07-13
  • 通讯作者: 赵琼玲
  • 基金资助:
    农业部热带作物种质资源保护项目“余甘子种质资源保护云南创新基地”(151721301354052003);金沙江干热河谷坝区生态综合治理及农 业产业发展技术试验示范(2017YFC0505102);“十二五”国家科技支撑项目子课题“云南干热区特色植物优异种质发掘创新与利用” (2013BAD01B00)。

Transcriptome Analysis of Phyllanthus emblica L.

  • Received:2018-02-03 Revised:2018-04-04 Accepted:2018-04-24 Online:2018-07-13 Published:2018-07-13

摘要: 为明确余甘子果实形成过程中基因表达的变化及药用性状形成的可能机制,本研究采用Hiseq2500PE125测序技术对4份余甘子种质进行转录组测序,在基本生物信息学分析及表达谱基因注解的基础上,对余甘子果实形成过程中药用性状相关功能基因进行预测。结果显示:余甘子果实包含192375个Transcript,47573个unigene,平均长度1140 bp;功能基因预测比对结果显示果实中碳代谢(Metabolish)相关的通路所占比例最多,达44.2%;氨基酸代谢(amino acid metabolism)通路441条,表明余甘子果实药用性状形成过程中碳代谢及氨基酸代谢活动较为强烈。基于余甘子药用成分为酚酸类化合物的共识,该结论为深入开展余甘子次生代谢物相关基因功能挖掘及调控机制研究奠定了基础。

关键词: 花生, 花生, 品种, 农艺性状, 比较分析

Abstract: To explore the changes of gene expression in Phyllanthus Emblica fruits and the possible mechanism of medical traits formation, transcriptomes of 4 Phyllanthus Emblica germplasms were sequenced by Hiseq2500PE125, based on the basic bioinformatics analysis and expression profile gene annotation, the relative functional genes of medical traits in Phyllanthus Emblica fruits were predicted. The results showedthat Phyllanthus Emblica fruits had 192375 transcripts, 47573 unigenes, and the average transcript length was 1140 bp. The functional gene predictions showed that the ways on carbon metabolism had the highest proportion in fruits, up to 44.2%, and amino acid metabolism pathways were 441, which revealed that the carbon metabolism and amino acid metabolism activity were strong in the forming process of fruit medicinal properties. Due to the consensus that the medicinal components of Phyllanthus Emblica germplasms are phenolic acid compounds, the conclusion provides references for the further studies on the functional genes and regulation of the secondary metabolites in Phyllanthus Emblica.