西藏灵菇来源酵母菌产谷胱甘肽能力比较及提取条件优化

doi:10.11924/j.issn.1000-6850.casb18020063

中国农学通报 ›› 2018, Vol. 34 ›› Issue (24): 77-81.doi: 10.11924/j.issn.1000-6850.casb18020063

所属专题：生物技术；食用菌；食用菌

西藏灵菇来源酵母菌产谷胱甘肽能力比较及提取条件优化

孟利,何静,刘峰,朱莹,张迷,李秀凉

黑龙江大学农业微生物技术教育部工程研究中心,黑龙江大学农业微生物技术教育部工程研究中心,黑龙江省农业科学院农产品质量安全研究所,黑龙江大学农业微生物技术教育部工程研究中心,黑龙江大学农业微生物技术教育部工程研究中心,黑龙江大学农业微生物技术教育部工程研究中心

收稿日期:2018-02-13 修回日期:2018-07-19 接受日期:2018-06-22 出版日期:2018-08-28 发布日期:2018-08-28
通讯作者: 李秀凉
基金资助:
哈尔滨市科技创新人才研究专项资金项目“西藏灵菇调节2 型糖尿病小鼠菌群关键菌株研究”(2015RQQXJ042)。

The Yeasts from Tibetan Kefir: Comparison of Glutathione Production and Extraction Optimization

Received:2018-02-13 Revised:2018-07-19 Accepted:2018-06-22 Online:2018-08-28 Published:2018-08-28

摘要/Abstract

摘要： 为获得高产谷胱甘肽菌株，采用DTNB法测定酵母菌体提取液中的GSH含量，比较了16株分离自西藏灵菇的酵母菌产谷胱甘肽能力，并以Y15为出发菌株，优化从菌体中提取谷胱甘肽条件。单因素试验比较了超声波、微波和热水三种细胞破碎方式，及菌体与提取液体积比、提取液pH、水浸提温度、时间对谷胱甘肽提取的影响，正交试验优化提取液pH、沸水浸提时间、料液比。结果表明，菌株Y19、Y15谷胱甘肽产量较高。采用Y15菌体冻融后热水抽提，料液比为1:6，pH2，沸水浴50min，其提取量最大，为49.90mg/L。Y15产谷胱甘肽较高，其谷胱甘肽提取条件为菌株Y15的利用提供了数据支持。

关键词: 胡麻饼, 胡麻饼, 营养成分, 瘤胃消失率

Abstract: To obtain the strains with high glutathione production, 16 yeasts from Tibetan Kefir were used as materials, GSH content in the yeast extract was detected with DTNB method. Taking Y15 as the original yeast, glutathione extraction condition was optimized by single factor experiment and the orthogonal test. The result showed that, yeast Y19 and Y15 had higher glutathione production. The optimized condition of glutathione extracted from Y15 was freeze-thaw hot water extraction, solid-liquid ratio 1:6, pH 2, and boiling water bath for 50 minutes. The yield of glutathione was 49.90 mg/L. Y15 is a high glutathione producing yeast, the glutathione extraction condition provides data basis for Y15 further utilization.

中图分类号:

TS202.3

孟利,何静,刘峰,朱莹,张迷,李秀凉. 西藏灵菇来源酵母菌产谷胱甘肽能力比较及提取条件优化[J]. 中国农学通报, 2018, 34(24): 77-81.

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西藏灵菇来源酵母菌产谷胱甘肽能力比较及提取条件优化

The Yeasts from Tibetan Kefir: Comparison of Glutathione Production and Extraction Optimization

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