关键词: 施氮量, 施氮量, 花生, 产质量, 氮肥利用率

Abstract: To evaluate the application effectiveness of various fecal examination methods in clinical preventive detection and case diagnosis of coccidiosis, Eimeria tenella sporulated oocysts were added to coccidia-negative feces of chickens for obtaining eight concentrations of oocysts as simulate clinical samples of coccidiosis. Then these fecal samples were examined and counted using the modified direct smear, sedimentation, saturated sodium chloride solution flotation, and modified McMaster egg counting methods. The results showed that the detection lower limit of modified direct smear technique was 6000 oocysts per gram of feces, that of fecal sedimentation method was 3000 oocysts per gram. A lot of debris were observed under microscope in these two methods. The detection lower limits of saturated sodium chloride solution flotation and modified McMaster egg counting methods were 100, 300 oocysts per gram, respectively. Small amount of debris was seen in both assays. The results of adding-recovering test for the modified McMaster egg counting method showed that the mean recovery rate of modified McMaster egg counting method was 82.2%, and mean coefficient of variation was 3.7%. 3-5 g was the optimal weight of fecal sample for the modified McMaster egg counting method. This technique is higher accuracy and reproducibility than other three methods in this study. Taken together, the modified McMaster egg counting method is the ideal and reliable method for detection of coccidian oocysts in veterinary clinical practice, and has huge value of popularization and application in control of coccidiosis in the intensive farms.