关键词: 火鹤, 火鹤, PDS 基因, 基因克隆, 表达分析

Abstract: To clarify the effect of consecutive application of biogas manure on maize yield, soil chemical property and benefit, a field experiment was conducted in Hailin Farm, Heilongjiang from May 2016 to October 2017, the treatments included biogas fluid, biogas residue and chemical fertilizers. The results showed that the application of biogas manure increased maize yield, the increasing rate under 15,000 kg/hm2 biogas fluid treatment was the highest, the treatment of 30000 kg/hm2 biogas fluid + 600 kg/hm2 chemical fertilizer had the highest maize yield. The application of 1t biogas manure could substitute 107.38 kg carbamide, 217.90 kg diammonium phosphate and 80.55 kg potassium sulfate. As a result, it generated a direct economic benefit of 826.16 yuan. Different treatments of biogas manures could achieve significant economic benefits compared with that of the control. The treatment of 30000 kg/hm2 biogas residue + 600 kg/hm2 chemical fertilizer had the highest economic benefit with an average amount of 19246.66 yuan/hm2. The pH values increased by 0.12 to 0.25 units and the organic matter, total nitrogen, total potassium and alkali hydrolysis nitrogen also increased in the surface layer of soil after the application of biogas manure. To some extent, the chemical properties of the surface layer of soil were improved. Therefore, the reasonable application of biogas manure can realize its efficient and economic utilization in biogas manure engineering areas.