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中国农学通报

中国农学通报 ›› 2019, Vol. 35 ›› Issue (23): 29-34.doi: 10.11924/j.issn.1000-6850.casb18050022

所属专题： 生物技术； 油料作物； 园艺

• 生物技术科学 • 上一篇    下一篇

菜用大豆蔗糖转运蛋白基因Glyma18g15950的 克隆及生物信息学分析

张玉梅, 胡润芳, 林国强   

  1. 福建省农业科学院作物研究所
  • 收稿日期:2018-05-07 修回日期:2019-07-17 接受日期:2018-07-25 出版日期:2019-08-13 发布日期:2019-08-13
  • 通讯作者: 林国强
  • 基金资助:
    福建省属公益类科研院所基本科研专项“菜用大豆蔗糖转运蛋白基因家族的克隆及表达分析”(2016R1025-6)，“大豆种质创新及育种技术 研究”（2018R1026-1)；福建省科技重大专项“粮食作物优质、高产、抗逆育种及关键技术研究”(2015NZ0002-3)；福建省农科院科技创新团队“特色旱 地作物创新团队”(STIT2017-2-6)。

Cloning and Bioinformatics Analysis of Sucrose Transporter Gene Glyma18g15950 in Vegetable Soybean

  • Received:2018-05-07 Revised:2019-07-17 Accepted:2018-07-25 Online:2019-08-13 Published:2019-08-13

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摘要： 为了从分子水平上揭示菜用大豆蔗糖转运蛋白的结构特点，我们从菜用大豆“闽豆6号”中同源克隆了蔗糖转运蛋白基因Glyma18g15950。经测序发现：该基因CDS长度为1794 bp，编码一个含有597个氨基酸的多肽。生物信息学分析表明，Glyma18g15950蛋白的等电点(pI)为5.81，分子量(Mw) 为64.30 kD；为疏水性蛋白。SOMPA分析表明，各个氨基酸残基对应的二级结构分别为α螺旋42.47%、β折叠13.71%、无规则卷曲39.63%和β转角4.18%；氨基酸序列和结构分析显示Glyma18g15950蛋白包含MFS_2结构域。SignalP分析表明该蛋白没有信号肽。系统进化树分析表明菜用大豆Glyma18g15950蛋白与野生大豆（Glycine soja）的亲缘关系最近，同源性达97.34%。将该蛋白与拟南芥的9个SUC蛋白比对表明Glyma18g15950与AtSUC3的亲缘关系最近，属于SUT2亚族。

关键词: 小麦, 小麦, 施肥, 品质, 氮磷钾肥, 微量元素, 有机肥

Abstract: In order to reveal the characteristics of vegetable soybean sucrose transporter in the molecular level, we homologously cloned the sucrose transporter gene Glyma18g15950 from the vegetable soybean variety “Mindou 6”. After sequencing, it was found that the length of CDS sequence was 1794 bp, encoding a polypeptide containing 597 amino acids. Bioinformatics analysis showed that the isoelectric point (pI) and molecular weight (Mw) was 5.81 and 64.30 kD, respectively, and this protein is a hydrophobic protein. The secondary structure of amino acid residues were also predicted by SOMPA, and the results showed 42.47% for α-helix, 13.71% for β-sheet, 39.63% for random coil and 4.18% for β-turn. Based to the amino acid sequence and structural analysis, Glyma18g15950 protein contained one conserved domain (MFS_2 domain). SignalP analysis showed that the protein has no signal peptide. Phylogenetic tree analysis showed that the Glyma18g15950 protein had the closest genetic relationship with Glycine soja and the homology was 97.34%. The alignment between this protein and 9 SUC proteins from Arabidopsis thaliana showed that Glyma18g15950 was closely to AtSUC3 and belonged to the SUT2 subfamily.