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中国农学通报

中国农学通报 ›› 2021, Vol. 37 ›› Issue (27): 132-138.doi: 10.11924/j.issn.1000-6850.casb2020-0729

• 食品·营养·检测·安全 • 上一篇    下一篇

正交法优化‘胭脂萝卜’红色素超声提取工艺

彭华1,2(), 宋霞2, 张春3()   

  1. 1四川旅游学院旅游文化产业学院,成都 610100
    2四川旅游学院旅游农业发展研究中心,成都 610100
    3西南医科大学药学院,四川泸州 646000
  • 收稿日期:2020-12-08 修回日期:2021-05-10 出版日期:2021-09-25 发布日期:2021-10-28
  • 通讯作者: 张春
  • 作者简介:彭华,男,1982年出生,四川泸州人,副研究员,博士,主要从事作物遗传育种和分子生物学育种研究。通信地址：610100 成都市龙泉驿区红岭路459号 四川旅游学院旅游文化产业学院,Tel：028-84825833,E-mail： penghua623@foxmail.com。
  • 基金资助:
    四川省科技厅应用基础项目“胭脂萝卜色素合成关键调控基因RsFLS及RsDFR功能及调控机理研究”(2020YJ0415);成都市科技局技术创新研发项目“果药间套栽培技术创新驱动都市休闲农业发展研究”(2019-YFYF-00167-SN);泸州市-西南医科大联合项目“紫花地丁有效成分积累受环境因素的调控规律及其分子机制研究”(2019LZXNYDJ49)

Optimization of Ultrasonic Extraction Process of Red Pigment from ‘Carmine Radish’ by Orthogonal Method

Peng Hua1,2(), Song Xia2, Zhang Chun3()   

  1. 1Department of Tourism and Cultural Industry, Sichuan Tourism University, Chengdu 610100
    2Research Center for Tourism Agricultural Development, Sichuan Tourism University, Chengdu 610100
    3Department of Pharmacology, School of Pharmacy, Southwest Medical University, Luzhou Sichuan 646000
  • Received:2020-12-08 Revised:2021-05-10 Online:2021-09-25 Published:2021-10-28
  • Contact: Zhang Chun

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摘要：

为了优化‘胭脂萝卜’红色素的提取工艺,以‘胭脂红2号’萝卜为原料,采用超声辅助提取法提取萝卜红色素,分析了提取液种类及配比、料液比、提取温度和提取时间等因素对‘胭脂萝卜’红色素提取液吸光值的影响。结果表明：（1）在试验条件下,盐酸丙酮提取的红色素溶液的吸光值最高。（2）提取液配比对吸光值影响达显著水平,料液比、提取温度对吸光值影响达极显著水平。对吸光值的影响从大到小依次为提取温度、料液比和提取液配比。（3）用优化后的提取条件测试不同萝卜品种的红色素含量,发现‘胭脂红1号’和‘胭脂红2号’红色素提取液的吸光值最高。综上,在提取料液比取1:8,提取温度取50℃,提取液选用盐酸丙酮提取液,其配比为10.8%的盐酸:30%的丙酮=1:1,提取时间选20 min的条件下,红色素提取液吸光值最高。

关键词: ‘胭脂萝卜’, 红色素, 超声提取, 正交设计, 吸光值

Abstract:

To optimize the extraction process of red pigment from ‘carmine radish’, the ‘Yanzhihong radish 2’ was used as the raw material to analyze the red pigment extraction yield under different solvents by using ultrasonic technology, and to study the effects of different types and ratios of extraction liquid, different solid-liquid ratios, extraction temperature and extraction time on the absorbance value. The results showed that: (1) the absorbance value of the red pigment solution extracted by acetone hydrochloride was the highest; (2) the effect of extraction liquid ratio on the absorbance value was significant, and the effects of solid-liquid ratio and extraction temperature on the absorbance value were extremely significant; the effects of extraction temperature, solid-liquid ratio and the ratio of extraction solution on the absorbance value were in a descending order; (3) the content of red pigment in different radish varieties were measured by the most optimal extraction condition, showing that the absorbance value of the red pigment extract of ‘Yanzhihong radish 1’ and ‘Yanzhihong radish 2’ were the highest. In general, the absorbance value of red pigment extraction is the highest under the condition of using acetone hydrochloride as the extracting solution, the ratio of 10.8% hydrochloric acid: 30% acetone is 1:1, the solid-liquid ratio is 1:8 (g/mL), the extraction temperature is 50℃ and the extracting duration is 20 min.

Key words: ‘carmine radish’, red pigment, ultrasonic extraction, orthogonal design method, absorbance value

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