不同pH条件下IBA和NAA对伴矿景天生根的影响研究

doi:10.11924/j.issn.1000-6850.casb20200200103

摘要/Abstract

摘要：

为解决酸性环境中伴矿景天扦插苗生根难的问题,以河沙为基质,研究不同pH条件对伴矿景天扦插生根的影响,并进一步探究不同浓度吲哚丁酸IBA和萘乙酸NAA处理对酸性逆境环境下伴矿景天扦插苗生根的影响。结果表明：pH 5.5时,扦插苗虽能正常生长,但长势欠佳,当pH≤5.0时,植株根系发育不良,茎细短、叶片小,生物量不到正常生长(pH 6.5)时的40%,且pH越低植株死亡越快;用0.5~10.0 mg/L浓度的IBA和NAA溶液浸泡伴矿景天扦插苗可显著提高扦插苗成活率,主要表现为生根时间短、根系长、数量多。研究表明,pH≤5.0的扦插环境不利于伴矿景天扦插苗生长,扦插前使用浓度分别为2.0~3.0 mg/L和1.0~2.0 mg/L的IBA和NAA溶液浸泡伴矿景天扦插穗基部,可缩短扦插苗在酸性环境中生根时间、增加根系数量和根系长度,提高成活率。

关键词: 伴矿景天, 扦插苗, pH, 生根, IBA, NAA

Abstract:

To solve the negative impacts of the acidic environment on rooting of Sedum plumbizincicola cutting seedlings, a sand culture experiment was conducted in artificial climate chamber. In this experiment, both the influence of different pH conditions on rooting and plant growth of cutting seedlings and the effects of different concentrations of IBA and NAA on the rooting of cutting seedlings were studied. The results showed that, although cutting seedlings could grow normally, they did not grow well at pH 5.5. At pH≤5.0, the root system was underdeveloped, the stems were shorter and the leaves were smaller, eventually resulting in less than 40% of the normal biomass (pH 6.5), and the lower the pH, the faster the plant would die. Soaking the cutting seedlings with 0.5-10.0 mg/L IBA and NAA could significantly improve the survival rate through shortening rooting time and increasing rooting number and length. This research suggests that culture medium with pH≤5.0 is harmful to Sedum plumbizincicola cutting seedlings’ growth. Soaking the base of Sedum plumbizincicola cutting seedlings with 2.0-3.0 mg/L IBA and 1.0-2.0 mg/L NAA before cuttage can shorten the rooting time, increase the number and the length of the root system, and improve the survival rate.

Key words: Sedum plumbizincicola, cutting seedlings, pH, rooting, IBA, NAA

中图分类号:

S318

贺爱国, 戴艳娇, 胡志鑫, 吴家梅, 陈锦. 不同pH条件下IBA和NAA对伴矿景天生根的影响研究[J]. 中国农学通报, 2020, 36(22): 49-54.

He Aiguo, Dai Yanjiao, Hu Zhixin, Wu Jiamei, Chen Jin. Effect of IBA and NAA on Root Development of Sedum plumbizincicola Under Different pH[J]. Chinese Agricultural Science Bulletin, 2020, 36(22): 49-54.

图/表 7

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pH	株高/cm	单株叶数/片	根长/cm	根数/条	生物量/(g/株)
4.0	7.13±0.42cC	21±2cC	0.17±0.06cC	2±0.58dD	0.0323±0.003dD
4.5	7.78±0.75cBC	21±2cC	0.20±0.10cC	5±2.59dD	0.0646±0.009cC
5.0	8.92±0.65bB	24±2bB	0.48±0.19cC	8±3.01dD	0.0624±0.007cCD
5.5	12.15±0.75aA	26±2bAB	1.72±0.34bB	18±4.34cC	0.1223±0.021bB
6.0	12.78±0.64aA	29±2aA	2.83±0.27aA	41±5.75bB	0.1495±0.017aAB
6.5	12.00±0.43aA	29±1aA	2.93±0.30aA	49±5.54aA	0.1648±0.006aA

pH	株高/cm	单株叶数/片	根长/cm	根数/条	生物量/(g/株)
4.0	7.13±0.42cC	21±2cC	0.17±0.06cC	2±0.58dD	0.0323±0.003dD
4.5	7.78±0.75cBC	21±2cC	0.20±0.10cC	5±2.59dD	0.0646±0.009cC
5.0	8.92±0.65bB	24±2bB	0.48±0.19cC	8±3.01dD	0.0624±0.007cCD
5.5	12.15±0.75aA	26±2bAB	1.72±0.34bB	18±4.34cC	0.1223±0.021bB
6.0	12.78±0.64aA	29±2aA	2.83±0.27aA	41±5.75bB	0.1495±0.017aAB
6.5	12.00±0.43aA	29±1aA	2.93±0.30aA	49±5.54aA	0.1648±0.006aA

pH	叶绿素a	叶绿素b	类胡萝卜素	叶绿素a+b
pH 4.0	1.80±0.36aA	0.55±0.12aA	0.40±0.08aA	2.35±0.48aA
pH 4.5	1.82±0.05aA	0.54±0.02aA	0.43±0.02aA	2.36±0.07aA
pH 5.0	1.13±0.08bB	0.32±0.03bB	0.26±0.02bB	1.45±0.11bB
pH 5.5	0.68±0.03cC	0.20±0.00cB	0.15±0.01cC	0.88±0.04cC
pH 6.0	0.62±0.05cC	0.18±0.01cB	0.13±0.02cC	0.80±0.07cC
pH 6.5	0.79±0.06cBC	0.27±0.05bcB	0.17±0.02cC	1.06±0.08cBC

pH	叶绿素a	叶绿素b	类胡萝卜素	叶绿素a+b
pH 4.0	1.80±0.36aA	0.55±0.12aA	0.40±0.08aA	2.35±0.48aA
pH 4.5	1.82±0.05aA	0.54±0.02aA	0.43±0.02aA	2.36±0.07aA
pH 5.0	1.13±0.08bB	0.32±0.03bB	0.26±0.02bB	1.45±0.11bB
pH 5.5	0.68±0.03cC	0.20±0.00cB	0.15±0.01cC	0.88±0.04cC
pH 6.0	0.62±0.05cC	0.18±0.01cB	0.13±0.02cC	0.80±0.07cC
pH 6.5	0.79±0.06cBC	0.27±0.05bcB	0.17±0.02cC	1.06±0.08cBC

激素	环境	0.0 mg/L	0.5 mg/L	1.0 mg/L	1.5 mg/L	2.0 mg/L	2.5 mg/L	3.0 mg/L	4.0 mg/L	6.0 mg/L	8.0 mg/L	10.0 mg/L
IBA	阴干	86.40bB	88.91aA	90.18aA	94.60aA	94.73aA	91.77aA	95.71aA	93.98aA	92.33aA	92.32aA	93.35aA
IBA	不阴干	79.35bB	84.18aA	89.55aA	90.90aA	94.27aA	93.26aA	92.92aA	93.59aA	91.24aA	91.57aA	90.23aA
NAA	阴干	86.79bB	93.72aA	92.40aA	93.39aA	94.71aA	92.73aA	94.38aA	93.39aA	94.05aA	93.72aA	90.42aA
NAA	不阴干	79.85bB	91.80aA	93.09aA	94.51aA	91.73aA	93.10aA	94.48aA	94.45aA	93.83aA	91.43aA	94.17aA