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中国农学通报

中国农学通报 ›› 2022, Vol. 38 ›› Issue (14): 53-59.doi: 10.11924/j.issn.1000-6850.casb2021-0570

所属专题： 生物技术； 园艺

• 林学·园艺·园林 • 上一篇    下一篇

不同年限唐古特瑞香不同药用部位活性物质初探

周志明1(), 吕彪1,4(), 闫芳2,4, 张春云3, 王海平3, 王勤礼2, 王建东3, 徐涛2, 张春梅2,3, 陈叶2   

  1. 1甘肃农业大学资源与环境学院,兰州 730000
    2河西学院祖师麻原植物应用技术研究所,甘肃张掖 734000
    3甘肃泰康制药有限责任公司,甘肃武威 733000
    4河西学院乡村振兴研究院,甘肃张掖 734000
  • 收稿日期:2021-05-31 修回日期:2021-09-16 出版日期:2022-05-15 发布日期:2022-06-07
  • 通讯作者: 吕彪
  • 作者简介:周志明,男,1997年出生,甘肃敦煌人,本科,研究方向：资源利用。通信地址：730000 甘肃省兰州安宁区营门村一号 甘肃农业大学资源与环境学院,E-mail： 969709419@qq.com。
  • 基金资助:
    甘肃省高等学校产业支撑计划项目“祖师麻药材绿色高效栽培技术研究与示范推广”(2021CYZC-58)

Preliminary Study on the Active Substances of Different Medicinal Parts of Daphne tangutica Maxim of Different Planting Years

ZHOU Zhiming1(), LV Biao1,4(), YAN Fang2,4, ZHANG Chunyun3, WANG Haiping3, WANG Qinli2, WANG Jiandong3, XU Tao2, ZHANG Chunmei2,3, CHEN Ye2   

  1. 1College of Resources and Environment, Gansu Agricultural University, Lanzhou 730000
    2Applied Technology Research Institute on Original Plants for Zushima of Hexi University, Zhangye, Gansu 734000
    3Taikang Pharmaceutical Limited Liability Company of Gansu Province, Wuwei, Gansu 733000
    4Research Institute for Rural Revitalization of Hexi University, Zhangye, Gansu 734000
  • Received:2021-05-31 Revised:2021-09-16 Online:2022-05-15 Published:2022-06-07
  • Contact: LV Biao

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摘要：

为对比不同地区、不同年限野生‘唐古特瑞香’根、茎中3种成分含量、变化规律,利用高效液相色谱仪(HPLC),应用ZORBAX SB-C18(4.6 mm ×150 mm)色谱柱,流动相使用乙腈︰0.05%磷酸(10：90)和甲醇︰水(20：80),流速为0.9 mL/min,检测波长为265 nm和327 nm,柱温为40℃,进样量为20 μL。建立的HPLC分析体系线性范围良好(R2>0.999),精密度、稳定性、重复性RSD均小于2%,加样回收率在95.10%~95.84%。含量结果分析表明,同一部位的样品,紫丁香苷的含量在第5年显著增加(P<0.05);互助地区根皮、茎皮和哈溪地区茎皮中祖师麻甲素、7-羟基香豆素,在第5年含量显著减少(P<0.05)。互助地区茎皮和哈溪地区根皮中祖师麻甲素与7-羟基香豆素含量次序分别为5年生>8年生>3年生,5年生>3年生>8年生(P<0.05)。同一年限的样品,除茎皮中祖师麻甲素、7-羟基香豆素和紫丁香苷含量显著高于根皮外(P<0.05),哈溪地区茎皮祖师麻甲素含量显著高于互助地区的根皮和茎皮含量(P<0.05);5年生和8年生祖师麻甲素与3年生7-羟基香豆素和紫丁香苷的含量依次为互助茎皮>哈溪茎皮>互助根皮>哈溪根皮(P<0.05);5年生和8年生紫丁香苷的含量依次为互助茎皮>互助根皮>哈溪茎皮>哈溪根皮(P<0.05)。‘唐古特瑞香’同一部位样品中多数成分含量在第5年较高;同一年份茎皮中多数成分含量高于根皮;哈溪地区5年生唐古特瑞香茎皮中成分含量最高。

关键词: 唐古特瑞香, 不同条件下, 高效液相, 含量测定

Abstract:

This study is to analyze the contents of three components and their changes in the rhizomes and stems of wild Daphne tangutica Maxim grown in different regions and of different planting years. High performance liquid chromatography (HPLC) and ZORBAX SB-C18 (4.6 mm ×150 mm) chromatographic column were used. The mobile phases of acetonitrile: 0.05% phosphoric acid (10:90) and methanol: water (20:80) were adopted. The flow rate was 0.9 mL/min, and the detection wavelength was 265 nm and 327 nm, respectively. The temperature was 40℃, and the sample load was 20 μL. The established HPLC system had good linear range (R2 > 0.999), the relative standard deviation (RSD) of precision, stability and repeatability were all less than 2%, and the recovery rate was 95.10%-95.84%. The content analysis showed that the content of syringin in the same part increased significantly in the fifth year (P< 0.05); the content of Daphentin and 7-hydroxycoumarin in the root bark and stem bark in Huzhu area and the stem bark in Haxi area decreased significantly in the fifth year (P< 0.05). The order of Daphentin and 7-hydroxycoumarin content in stem bark in Huzhu area and root bark in Haxi area was 5-year-old > 8-year-old > 3-year-old and 5-year-old > 3-year-old > 8-year-old, respectively (P< 0.05). For the same year samples, except that the content of Daphentin, 7-hydroxycoumarin and syringin in stem bark were significantly higher than those in root bark (P< 0.05), the content of Daphentin in stem bark in Haxi area was significantly higher than that in root bark and stem bark in Huzhu area (P< 0.05). The content of Daphentin of 5-year-old and 8-year-old and the content of 7-hydroxycoumarin and syringin of 3-year-old were in the order of Huzhu stem bark > Haxi stem bark > Huzhu root bark > Haxi root bark (P< 0.05); the content of syringin of 5-year-old and 8-year-old were in the order of Huzhu stem bark > Huzhu root bark > Haxi stem bark > Haxi root bark (P< 0.05). The content of most components in the same part of Daphne tangutica Maxim were higher in the fifth year; and the content of most components in stem bark was higher than that in root bark in the same year plant; the content of components in the stem bark of 5-year-old Daphne tangutica Maxim in Haxi area was the highest.

Key words: Daphne tangutica Maxim, under different conditions, high performance liquid chromatography, content determination

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