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中国农学通报

中国农学通报 ›› 2023, Vol. 39 ›› Issue (9): 123-127.doi: 10.11924/j.issn.1000-6850.casb2022-0296

• 植物保护·农药 • 上一篇    下一篇

不同药剂对松褐天牛固氮活性影响的研究

金明霞1(), 王洋1, 谢谷艾1, 王占印2, 熊彩云1, 刘晓华1, 涂业苟1, 万静1, 严员英1   

  1. 1 江西省林业科学院，南昌 330013
    2 江西省森林资源保护中心，南昌 330038
  • 收稿日期:2022-04-14 修回日期:2022-06-16 出版日期:2023-03-25 发布日期:2023-03-23
  • 作者简介:

    金明霞，女，1979年出生，副研究员，硕士，研究方向：主要从事昆虫资源开发研究。通信地址：江西省南昌市经济技术开发区枫林西大街1629号，Tel：15679190036，E-mail：。

  • 基金资助:
    江西省科技厅重点研发项目“基于松褐天牛氮素营养机制的防治技术研究”(20202BBFL63016); 江西林业科技创新专项“油茶蓝翅天牛引诱剂的开发与利用技术研究”([2021]26号); 江西省科技厅重点研发项目“无人机高光谱遥感松萎蔫病潜伏树技术研发及集成应用”(20202BBFL63045); 江西林业科技创新专项“龙虎山风景区松材线虫病防控技术应用研究”([2021]31号); 2022年中央财政林业科技推广示范项目“2.3%甲维盐注干液剂防治松材线虫病应用示范与推广”(JXTG[2022]24号)

Effects of Different Chemicals on Nitrogenase Activity of Monochamus alternatus Hope

JIN Mingxia1(), WANG Yang1, XIE Gu'ai1, WANG Zhanyin2, XIONG Caiyun1, LIU Xiaohua1, TU Yegou1, WAN Jing1, YAN Yuanying1   

  1. 1 Jiangxi Academy of Forestry, Nanchang 330013
    2 Jiangxi Forest Resources Protection Center, Nanchang 330038
  • Received:2022-04-14 Revised:2022-06-16 Online:2023-03-25 Published:2023-03-23

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摘要：

目前，松褐天牛是国内外林业有害生物防治中的一个难题，基于松褐天牛为食木昆虫，具有自身固氮能力，通过筛选出对松褐天牛固氮活性具有较大影响的药剂，来破坏其固氮能力导致松褐天牛缺乏氮素营养而死亡，从而来开辟新的防治途径。本研究采用乙炔还原法及酶联免疫试剂盒测定了不同药剂处理下的松褐天牛肠道固氮菌和不同生长阶段（成虫、幼虫及卵）虫体的固氮酶活性差异，结果表明，阿维菌素和NH4Cl处理对松褐天牛成虫固氮酶活性的影响较大，在药剂处理下的固氮活性仅为0.1 IU/g和0.08 IU/g，与对照相比固氮酶活性分别减少1.82 IU/g和1.85 IU/g；而固氮活性最强的菌株EH在NH4Cl处理下的固氮活性仅为1.2 nmol/(mL·h)，比正常情况下的固氮活性减少了50.9 nmol/(mL·h)。因此，理论上NH4Cl可应用于林间防治松褐天牛。

关键词: 松褐天牛, 固氮, 活性, 药剂

Abstract:

At present, Monochamus alternatus is a challenge in the prevention and control of forestry pests at home and abroad. Based on the fact that Monochamus alternatus is a wood-feeding insect and has its own nitrogen-fixing ability, the nitrogen fixation ability of Monochamus alternatus can be destroyed by screened chemicals, resulting in the death of Monochamus alternatus due to the lack of nitrogen nutrition. This is a new way of pest control. The authors used acetylene reduction method and enzyme-linked immunosorbent assay kit, to determine the difference of nitrogenase activity between intestinal nitrogenase bacteria of Monochamus alternatus and insects at different growth stages (adults, larvae and eggs) under different chemical treatments. The results showed that the treatment of abamectin and NH4Cl had great influence on nitrogenase activity of Monochamus alternatus adults. The nitrogenase activity under both the two chemical treatments was only 0.1 IU/g and 0.08 IU/g, respectively. Compared with that of the control, the nitrogenase activity was decreased by 1.82 IU/g and 1.85 IU/g, respectively. Strain EH had the strongest nitrogen fixation activity, but its nitrogen fixation activity was only 1.2 nmol/(mL·h) under NH4Cl treatment, 50.9 nmol/(mL·h) less than the normal nitrogen fixation activity. In theory, NH4Cl can be used to control Monochamus alternatus in forests.

Key words: Monochamus alternatus, nitrogen fixation, activity, chemicals