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中国农学通报

中国农学通报 ›› 2023, Vol. 39 ›› Issue (32): 150-157.doi: 10.11924/j.issn.1000-6850.casb2022-0884

• 畜牧·动物医学·蚕·蜂 • 上一篇    下一篇

基于RNA-Seq技术分析不同产蛋量庄河大骨鸡垂体和下丘脑的可变剪接事件

马智勇(), 李美成, 马巍, 王春强()   

  1. 锦州医科大学畜牧兽医学院，辽宁锦州 121001
  • 收稿日期:2022-10-25 修回日期:2023-04-05 出版日期:2023-11-15 发布日期:2023-11-10
  • 通讯作者: 王春强，男，1980年出生，山东聊城人，教授，博士，主要从事动物遗传繁育方面的研究。通信地址：121001 辽宁省锦州市古塔区人民街5段48号 锦州医科大学西校区，Tel：0416-2625901，E-mail：wcqworld@126.com。
  • 作者简介:

    马智勇，男，1998年出生，贵州遵义人，研究方向：动物科学。通信地址：121001 辽宁省锦州市古塔区人民街5段48号 锦州医科大学西校区，E-mail：。

  • 基金资助:
    国家级大学生创新训练项目“NGF-FSH协同调节ABP表达对睾丸支持细胞的作用的初步研究”(201910160012); 省级大学生创新训练项目“N-氨基甲酰谷氨酸通过调节NO代谢影响热应激大骨鸡精液品质的研究”(S202110160020); 辽宁省教育厅服务地方项目“基于辽宁地方保护品种大骨鸡选育高端蛋用品系的分子标记辅助育种技术开发与应用”(JYTFW2020008)

Alternative Splicing Events in Pituitary and Hypothalamus of Zhuanghe Dagu Chickens with Different Egg Production Levels: Analysis Based on RNA-Seq Technology

MA Zhiyong(), LI Meicheng, MA Wei, WANG Chunqiang()   

  1. School of Animal Husbandry and Veterinary Medicine, Jinzhou Medical University, Jinzhou, Liaoning 121001
  • Received:2022-10-25 Revised:2023-04-05 Published-:2023-11-15 Online:2023-11-10

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摘要：

近年来，庄河大骨鸡个体产蛋数量出现较大差异。可变剪接(Alternative splicing，AS)是真核基因转录后调控的重要机制，但目前尚无关于AS与禽垂体和下丘脑产蛋量相关性的研究。本试验基于RNA-Seq技术对大骨鸡AS事件进行分析。结果表明：在垂体和下丘脑文库中分别检测到769和813个差异AS事件。2种文库（垂体、下丘脑）中均鉴定出5种类型的AS事件，其中外显子跳跃(SE)的比例最高，而替代5'剪接位点(A5SS)的比例最低；NRCAM，SLMAP和CIB1基因的AS发生频率较高；2种组织文库中分别筛选出644和680个差异剪接基因(DSGs)，其中SE的比例最高，A5SS的比例最低。GO和KEGG富集分析分别对329个和337个DSG进行功能注释；RT-PCR检测发现，ESR1存在于不同的AS事件中；12个文库中共获得6758个新的转录本，优化了12486个基因。研究结果不仅扩展了对家禽垂体和下丘脑组织中AS事件的认识，且为研究生产中提高鸡的产蛋量提供新的思路。

关键词: 鸡, 可变剪接, RNA-Seq, 差异剪接基因, 产蛋量

Abstract:

In recent years, there have been great differences in the number of eggs laid by individuals of Zhuanghe Dagu chickens. Alternative splicing (AS) is an important mechanism of post-transcriptional regulation of eukaryotic genes, but there is no research on the correlation between AS and egg production in avian pituitary and hypothalamus. Based on RNA-Seq technology, this experiment analyzed the AS events of Zhuanghe Dagu chickens. The results showed that 769 and 813 differential AS events were detected in the pituitary and hypothalamic libraries, respectively. Five types of AS events were identified in both libraries (pituitary, hypothalamus), with the highest proportion of exon skipping (SE) and the lowest proportion of alternative 5' splice site (A5SS); NRCAM, SLMAP and CIB1 gene had a higher frequency of AS; 644 and 680 differentially spliced genes (DSGs) were screened from the two tissue libraries, respectively, with the highest proportion of SE and the lowest proportion of A5SS. GO and KEGG enrichment analysis annotated 329 and 337 DSGs, respectively; RT-PCR detection found that ESR1 was present in different AS events; a total of 6758 new transcripts were obtained from 12 libraries, and 12486 gene were optimized. The findings not only expand the understanding of AS events in poultry pituitary and hypothalamic tissues, but also provide new ideas for improving chicken egg production in research and production.

Key words: chicken, alternative splicing, RNA-Seq, differentially spliced genes, egg production