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中国农学通报

中国农学通报 ›› 2023, Vol. 39 ›› Issue (31): 76-82.doi: 10.11924/j.issn.1000-6850.casb2022-0980

• 林学·园艺·园林 • 上一篇    下一篇

圆锥绣球‘花园蕾丝’组培快繁技术研究

邓衍明1,2(), 秦紫艺1,2, 陈双双1, 邱帅3, 冯景1, 高凯3, 齐香玉1, 陈慧杰1   

  1. 1 江苏省农业科学院休闲农业研究所/江苏省高效园艺作物遗传改良重点实验室，南京 210014
    2 南京农业大学园艺学院，南京 210008
    3 杭州市园林绿化股份有限公司，杭州 310009
  • 收稿日期:2022-11-23 修回日期:2023-08-15 出版日期:2023-10-31 发布日期:2023-10-31
  • 作者简介:

    邓衍明，男，1976年出生，安徽滁州人，研究员，博士，研究方向为花卉遗传育种、栽培技术与分子生物学。通信地址：210014 江苏省南京市玄武区钟灵街50号 省农科院休闲农业研究所，Tel：025-84399306，E-mail：。

  • 基金资助:
    江苏省农业科技自主创新资金项目“基于休闲农业的绣球花特色资源挖掘与综合利用关键技术研究”(CX(22)2035); 江苏省林业科技创新与推广项目“绣球耐寒新品种引选与花色定制产品培育利用研究”(LYKJ-沭阳(2021)01); 国家林草局林草国家创新联盟重点研发项目“绣球种质资源评价、创制与产业化开发”(GLM(2021)86号)

Study on Tissue Culture Technology and Rapid Propagation of Hydrangea paniculata ‘Garden Lace’

DENG Yanming1,2(), QIN Ziyi1,2, CHEN Shuangshuang1, QIU Shuai3, FENG Jing1, GAO Kai3, QI Xiangyu1, CHEN Huijie1   

  1. 1 Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement/ Institute of Leisure Agriculture, Jiangsu Academy of Agricultural Sciences, Nanjing 210014
    2 College of Horticulture, Nanjing Agricultural University, Nanjing 210008
    3 Hangzhou Landscaping Incorporated, Hangzhou 310009
  • Received:2022-11-23 Revised:2023-08-15 Published-:2023-10-31 Online:2023-10-31

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摘要：

以圆锥绣球‘花园蕾丝’的带芽茎段为外植体，研究外植体消毒方法、基本培养基与植物生长调节剂组合对其腋芽萌发、丛生芽增殖和不定根诱导的效果，以及不同基质对组培苗移栽的影响。以不同有效氯浓度的次氯酸钠溶液消毒不同时间处理进行消毒效果比较，并以MS、1/2MS、WPM、1/2WPM等作为基本培养基，添加不同浓度的6-BA、NAA或IBA进行侧芽诱导、增殖和生根；以珍珠岩、蛭石、泥炭配比成不同的基质，进行组培苗的移栽。结果表明，‘花园蕾丝’外植体最佳消毒方法是75%乙醇消毒30 s后，经无菌水冲洗3~4次，再用有效氯浓度2.0%的次氯酸钠溶液消毒8~10 min，最后用无菌水冲洗4~6次；腋芽萌发最佳培养基为1/2WPM+ 2.0 mg/L 6-BA，芽增殖的最佳培养基为WPM + 2.0 mg/L 6-BA+ 0.2 mg/L IBA，诱导生根的最佳培养基为1/2WPM+ 0.3~0.5 mg/L IBA，组培苗移栽最佳基质为按等体积比混匀的珍珠岩、蛭石和泥炭土。研究初步建立了圆锥绣球‘花园蕾丝’的组培快繁技术体系，可为其工厂化育苗和规模化生产提供技术支持。

关键词: 圆锥绣球, 离体培养, 快繁技术, 组培苗, 外植体消毒, 炼苗移栽

Abstract:

The stem segments of Hydrangea paniculata ‘Garden Lace’ with axillary buds were used as explants to study the sterilization protocol, the effects of basic culture mediums and plant growth regulators on axillary buds germination, multiple shoot proliferation and adventitious root induction, and the influence of different substrates on the transplanting of tissue culture seedlings. The sterilization effects of NaClO at various chlorine concentrations and treatment times were compared. The treated axillary buds induction, proliferation, and rooting were taken on the basic mediums of MS, 1/2MS, WPM, and 1/2WPM, with different concentrations of 6-BA, NAA, or IBA. The generated tissue culture seedlings were transplanted into various substrates that were mixed with peat, perlite, and vermiculite. The results showed that the most suitable sterilization method was to soak the explant in 75% ethanol for 30 s, followed by 3-4 washings with sterile water. After that, explants were treated with NaClO of 2.0% available chlorine concentration for 8-10 min, followed by 4-6 washings with sterile water. The best start-up medium for axillary buds was 1/2WPM+ 2.0 mg/L 6-BA, for shoots was WPM+ 2.0 mg/L 6-BA+ 0.2 mg/L IBA, and for rooting was 1/2WPM+ 0.3-0.5 mg/L IBA. The best substrate for transplanting the plantlets was an equal amount of well-mixed perlite, vermiculite, and peat. The study developed a quick propagation system for H. paniculata ‘Garden Lace’ through tissue culture, and provided a technical guidance for factory-scale seedling development and mass production.

Key words: Hydrangea paniculata, culture in vitro, rapid propagation technology, tissue culture seedling, explant sterilization, transplant seedling