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中国农学通报 ›› 2023, Vol. 39 ›› Issue (27): 140-148.doi: 10.11924/j.issn.1000-6850.casb2022-0988

• 植物保护·农药 • 上一篇    下一篇

盐碱地冬枣内生真菌的分离鉴定及抑菌活性研究

雷早娟1(), 武亚明1, 刘子欢1, 安鹏天1, 娄巧哲2, 窦华云1   

  1. 1 石家庄海关技术中心黄骅港业务部,河北沧州 061113
    2 石家庄海关技术中心,石家庄 050051
  • 收稿日期:2022-11-28 修回日期:2023-03-15 出版日期:2023-09-25 发布日期:2023-09-22
  • 作者简介:

    雷早娟,女,1991年出生,农艺师,硕士研究生,研究方向:植物病理学,主持完成省部级科研项目1项。通信地址:061113 河北省沧州市渤海新区黄骅港海关,Tel:0317-5767965,E-mail:

  • 基金资助:
    河北省科技计划项目(18226515)

Endophytes of Ziziphus jujube: Isolation, Identification and Antibacterial Activity in Saline-Alkali Field

LEI Zaojuan1(), WU Yaming1, LIU Zihuan1, AN Pengtian1, LOU Qiaozhe2, DOU Huayun1   

  1. 1 Business Department of Huanghua Port, Technical Center of Shijiazhuang Customs District, Cangzhou, Hebei 061113
    2 Technical Center of Shijiazhuang Customs District, Shijiazhuang 050051
  • Received:2022-11-28 Revised:2023-03-15 Published-:2023-09-25 Online:2023-09-22

摘要:

为了研究盐碱地冬枣内生真菌的组成及其抑菌活性,以黄骅冬枣的健康组织为供试材料,采用组织分离、真菌形态学和ITS序列分析的方法,从冬枣不同组织分离到111个菌株,鉴定为19个属共计23种,其中优势菌群为链格孢属(Alternaria)和镰刀菌属(Fusarium),相对分离频率分别为37.84%和21.62%。冬枣各个组织内生真菌的多样性存在明显差异,枝条内生真菌的Shannon-Weaver指数(H=2.02)远高于花(0.80)。通过平板对峙法筛选出3株对冬枣黑斑病菌(Alternaria tenuissima)和梨轮纹病菌(Botryosphaeria dothidea)具有抑制作用的内生真菌,其中菌株J7-4-4及其发酵液对2种靶标菌的抑菌活性最强。本试验首次研究盐碱地冬枣内生真菌及其抑菌活性,为特殊生长环境下植物内生真菌的研究及开发提供理论依据。

关键词: 冬枣, 盐碱地, 内生真菌, 多样性, 抑菌活性

Abstract:

To investigate the community composition and antibacterial activity of endophytes of Ziziphus jujube in saline-alkali field, the healthy tissues of Z. jujube in Huanghua were used as test materials. Tissue isolation method, morphology and internal transcribed spacer (ITS) sequence analysis were used to obtain 111 endophytes, affiliated with 19 genera and 23 species, among which Alternaria and Fusarium were the dominant genus with the relative isolation frequency of 37.84% and 21.62%. There were significant differences in the diversity of endophytes in various tissues of Z. jujube, and the Shannon Weaver index (H=2.02) of endophytes in branches was much higher than that in flowers (0.80). The three strains of endophytes with antibacterial effect on both Alternaria tenuissima and Botryosphaeria dothidea were found by plate confrontation method, among which the strain J7-4-4 and its products showed higher antibacterial effect on the two target fungi. We studied endophytes and antibacterial activity of Z. jujube in saline-alkali field for the first time, which provided theoretical basis for the study and development of endophytes in plants under special growing environment.

Key words: Ziziphus jujube, saline-alkali field, fungal endophytes, diversity, antibacterial activity