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中国农学通报

中国农学通报 ›› 2023, Vol. 39 ›› Issue (12): 54-60.doi: 10.11924/j.issn.1000-6850.casb2022-0313

• 资源·环境·生态·土壤 • 上一篇    下一篇

南水北调东线6个湖泊铜锈环棱螺遗传多样性与遗传结构分析

赵燕1(), 张冲1, 崔梦瑶1, 李娴2, 路广金1, 季相山1, 陈红菊1()   

  1. 1 山东农业大学动物科技学院，山东泰安 271018
    2 山东省淡水渔业研究院，济南 250117
  • 收稿日期:2022-04-28 修回日期:2022-08-27 出版日期:2023-04-25 发布日期:2023-04-21
  • 通讯作者: 陈红菊，女，1972年出生，河北任丘人，副教授，博士，研究方向：水产动物种质资源保护与遗传育种。通信地址：271018 山东省泰安市岱宗大街61号，山东农业大学动物科技学院，Tel：0538-8241544-8107，E-mail：hjchen72@sdau.edu.cn。
  • 作者简介:

    赵燕，女，1979年出生，山东高唐人，副教授，博士，研究方向：水产动物遗传育种。通信地址：271018 山东省泰安市岱宗大街61号，山东农业大学动物科技学院，Tel：0538-8241544-8107，E-mail：。

  • 基金资助:
    山东省农业良种工程项目“水产生物种质资源收集保护与精准鉴定”(2019LZGC020); 山东省重点研发计划（重大科技创新工程）项目“水产种质资源挖掘与精准鉴定”(2021LZGC027); “重要经济鱼类品种创制与绿色养殖关键技术研究与示范”(2021CXGC010806)

Bellamya aeruginosa in Six Lakes Along the East Line of the South-to-North Water Diversion Project: Analysis of Genetic Diversity and Genetic Structure

ZHAO Yan1(), ZHANG Chong1, CUI Mengyao1, LI Xian2, LU Guangjin1, JI Xiangshan1, CHEN Hongju1()   

  1. 1 College of Animal Science and Technology, Shandong Agricultural University, Tai’an, Shandong 271018
    2 Shandong Freshwater Fisheries Research Institute, Jinan 250117
  • Received:2022-04-28 Revised:2022-08-27 Online:2023-04-25 Published:2023-04-21

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摘要：

为评价调水工程对输水沿线湖泊水环境生态的影响，以高原湖泊滇池群体作对照，以南水北调东线各水域：东平湖、南四湖（微山湖）、骆马湖、洪泽湖、高邮湖、长江（扬州江都区）水域野生铜锈环棱螺为材料，基于COI和16S rRNA基因分析了各群体的遗传多样性与系统发育关系。结果显示，滇池群体单倍型多样性(0.924，0.958)、核苷酸多样性(0.04466，0.03068)、平均核苷酸差异数(27.333，14.117)均远高于南水北调东线6个群体。Tajima's中性检验结果均不显著偏离中性(P>0.10)，核苷酸配对差异分布图并不呈现明显的单峰。滇池与东线6个群体遗传距离远远大于6个群体间遗传距离。聚类结果显示，南水北调东线6个群体大部分个体相互混杂在一起，而滇池群体的大部分个体集中在一起，与其他群体偶有混杂。研究表明，调水工程对沿线水域铜锈环棱螺的遗传多样性与遗传结构均产生了影响。

关键词: 南水北调, 铜锈环棱螺, COI, 16S rRNA, 遗传多样性, 遗传结构

Abstract:

In order to evaluate the impact of the South-to-North Water Diversion Project on water environment and ecology of lakes along the water conveyance route, using wild Bellamya aeruginosa in different lakes and water area (Dongping Lake, Nansi Lake, Luoma Lake, Hongze Lake, Gaoyou Lake etc.) along the east line of the project as the materials, and that in Dianchi Lake as the control, the genetic diversity and phylogenetic relationship of various populations were analyzed based on COI and 16S rRNA genes. The results showed that the haplotype diversity (0.924, 0.958), nucleotide diversity (0.04466, 0.03068) and average nucleotide diversity (27.333, 14.117) of Dianchi Lake population were much higher than those of the six populations along the east line of the South-to-North Water Diversion Project. Tajima’s neutral test results did not deviate from the neutral significantly (P>0.10), and the nucleotide pairing difference distribution map did not show an obvious single peak. The genetic distance between Dianchi Lake population and the six populations along the east line was much greater than that among the six populations. The cluster analysis showed that most individuals of the six populations along the east line of the South-to-North Water Diversion Project were mixed together, while most individuals of the Dianchi Lake population were concentrated together, mixing with other populations occasionally. The study indicates that the water diversion project has an impact on the genetic diversity and genetic structure of Bellamya aeruginosa in waters along the project route.

Key words: South-to-North Water Diversion Project, Bellamya aeruginosa, COI, 16S rRNA, genetic diversity, genetic structure