安全剂环丙磺酰胺和双苯噁唑酸对玉米苯唑氟草酮药害的解毒作用

doi:10.11924/j.issn.1000-6850.casb2023-0221

中国农学通报 ›› 2023, Vol. 39 ›› Issue (23): 88-93.doi: 10.11924/j.issn.1000-6850.casb2023-0221

所属专题：生物技术；植物保护

安全剂环丙磺酰胺和双苯噁唑酸对玉米苯唑氟草酮药害的解毒作用

连磊¹(), 王恒智²^,³, 刘伟堂²^,³, 张耀中⁴, 金岩⁴, 冯克⁵, 黄义召⁶, 陈爽¹, 路兴涛¹, 高传杰⁴()

¹青岛清原作物科学有限公司，山东青岛 266000
²山东农业大学植物保护学院，山东泰安 271018
³山东省农药毒理与应用技术重点实验室，山东泰安 271018
⁴山东省农药检定所，济南 250000
⁵聊城市农业技术推广服务中心，山东聊城 252000
⁶枣庄学院，山东枣庄 277000

收稿日期:2023-03-20 修回日期:2022-05-24 出版日期:2023-08-15 发布日期:2023-08-10
作者简介:
连磊，男，1984年出生，山东青岛人，高级农艺师，博士研究生，主要从事新农药创制及推广应用方面的研究。通信地址：266000 青岛市黄岛区青龙河路53号青岛清原作物科学有限公司，E-mail：lianlei@kingagroot.com。
基金资助:
山东省重点研发计划（重大科技创新工程）项目“生态友好无公害重大绿色农药品种创制及产业化”(2021CXGC010811)

The Detoxification Effect of Safeners of Cyclopropanesulfonamide and Isoxadifen-ethyl against Fenpyrazone in Corn

LIAN Lei¹(), WANG Hengzhi²^,³, LIU Weitang²^,³, ZHANG Yaozhong⁴, JIN Yan⁴, FENG Ke⁵, HUANG Yizhao⁶, CHEN Shuang¹, LU Xingtao¹, GAO Chuanjie⁴()

¹Qingdao KingAgroot Crop Science Co., Ltd., Qingdao, Shandong 266000
²College of Plant Protections, Shandong Agricultural University, Tai’an, Shandong 271018
³Key Laboratory of Pesticide Toxicology and Application Technology, Tai’an, Shandong 271018
⁴Institute for the Control of Agrochemicals of Shandong Province, Jinan 250000
⁵Liaocheng Agricultural Technology Extension Service Center, Liaocheng, Shandong 252000
⁶Zaozhuang University, Zaozhuang, Shandong 277000

Received:2023-03-20 Revised:2022-05-24 Published:2023-08-15 Online:2023-08-10

摘要/Abstract

摘要：

为了优化苯唑氟草酮在玉米田的应用技术。以‘辽单127’玉米(Zea mays Linn.)为测试靶标，采用温室盆栽法研究了安全剂环丙磺酰胺和双苯噁唑酸对玉米体内苯唑氟草酮的解毒效果，并通过对谷胱甘肽转移酶(GSTs)活性的测定初步研究了环丙磺酰胺的解毒机理。结果显示：在苯唑氟草酮有效成分量360 g/hm²茎叶喷雾处理下，7.5、15、30 g/hm²的环丙磺酰胺和双苯噁唑酸均可提高‘辽单127’玉米对苯唑氟草酮的耐药性，株高和鲜重保护率在117%~269%之间。其中，加入环丙磺酰胺15 g/hm²的苯唑氟草酮处理组的株高和鲜重保护率在所有处理组中均达到了最高，株高保护率为133%，鲜重保护率为269%。添加安全剂环丙磺酰胺15 g/hm²后，苯唑氟草酮处理的玉米GSTs比活力在施药后1~9 d内均显著提高，在施药后第5天，GSTs比活力达到最大。结果表明添加安全剂环丙磺酰胺提高了玉米体内GST活性，降低了苯唑氟草酮对玉米生长的抑制作用，减轻了苯唑氟草酮对玉米的药害。

关键词: 除草剂, 安全剂, 玉米, 苯唑氟草酮, 环丙磺酰胺

Abstract:

In order to optimize the application technology of fenpyrazone in corn (Zea mays Linn.) fields, the detoxification effect of safeners cyclopropanesulfonamide and isoxadifen-ethyl on fenpyrazone was studied in greenhouse targeted by ‘Liaodan 127’ maize, and the detoxification mechanism of cyclopropanesulfonamide was preliminarily studied by measuring the activity of glutathione transferase (GSTs) in maize. The results showed that under the treatment of effective component of fenpyrazone 360 g (a.i.) /hm² stem and leaf spray, 7.5, 15, 30 g a.i. /hm²of cyclopropanesulfonamide and isoxadifen-ethyl could improve the tolerance of ‘Liaodan 127’ maize to fenpyrazone, and the protection rate of plant height and fresh weight was between 117% and 269%. Among them, the protection rate of plant height and fresh weight of the treatment group treated with fenpyrazone combined with cyclopropanesulfonamide 15 g a.i. /hm² reached the highest, with the protection rate of plant height and fresh weight of 133% and 269%, respectively. After adding the cyclopropanesulfonamide at 15 g a.i. /hm², the enzymatic specific activity of GSTs in maize treated with fenpyrazone increased significantly within 1-9 days after application, and reached the maximum on the 5th day after application. In conclusion, cyclopropanesulfonamide increased the GSTs activity in maize, reduced the inhibitory effect of fenpyrazone on maize growth, and alleviated the herbicide damage in maize caused by fenpyrazone.

Key words: herbicide, safener, maize, fenpyrazone, cyclopropanesulfonamide

连磊, 王恒智, 刘伟堂, 张耀中, 金岩, 冯克, 黄义召, 陈爽, 路兴涛, 高传杰. 安全剂环丙磺酰胺和双苯噁唑酸对玉米苯唑氟草酮药害的解毒作用[J]. 中国农学通报, 2023, 39(23): 88-93.

LIAN Lei, WANG Hengzhi, LIU Weitang, ZHANG Yaozhong, JIN Yan, FENG Ke, HUANG Yizhao, CHEN Shuang, LU Xingtao, GAO Chuanjie. The Detoxification Effect of Safeners of Cyclopropanesulfonamide and Isoxadifen-ethyl against Fenpyrazone in Corn[J]. Chinese Agricultural Science Bulletin, 2023, 39(23): 88-93.

图/表 3

参考文献 38

[1]	WANG H, WANG L, ZHANG X, et al. Unravelling phytotoxicity and mode of action of tripyrasulfone, a novel herbicide[J]. Journal of agricultural and food chemistry, 2021, 69(25):7168-7177. doi: 10.1021/acs.jafc.1c01294 pmid: 34152147
[2]	GALLEGO S M, PENA L B, BARCIA R A, et al. Unravelling cadmium toxicity and tolerance in plants: Insight into regulatory mechanisms[J]. Environmental and experimental botany, 2012, 83:33-46. doi: 10.1016/j.envexpbot.2012.04.006 URL
[3]	XU K, RACINE F, HE Z, et al. Impacts of hydroxyphenylpyruvate dioxygenase (HPPD) inhibitor (mesotrione) on photosynthetic processes in Chlamydomonas reinhardtii[J]. Environmental pollution, 2019, 244:295-303. doi: 10.1016/j.envpol.2018.09.121 URL
[4]	LIU J, GERKEN H, HUANG J, et al. Engineering of an endogenous phytoene desaturase gene as a dominant selectable marker for Chlamydomonas reinhardtii transformation and enhanced biosynthesis of carotenoids[J]. Process biochemistry, 2013, 48(5-6):788-795. doi: 10.1016/j.procbio.2013.04.020 URL
[5]	NORRIS S R, BARRETTE T R, DELLAPENNA D. Genetic dissection of carotenoid synthesis in arabidopsis defines plastoquinone as an essential component of phytoene desaturation[J]. The plant cell, 1995, 7(12):2139-2149.
[6]	QIN G, GU H, MA L, et al. Disruption of phytoene desaturase gene results in albino and dwarf phenotypes in Arabidopsis by impairing chlorophyll, carotenoid, and gibberellin biosynthesis[J]. Cell Research, 2007, 17(5):471-482. doi: 10.1038/cr.2007.40 pmid: 17486124
[7]	HAVAUX M, EYMERY F, PORFIROVA S, et al. Vitamin E protects against photoinhibition and photooxidative stress in Arabidopsis thaliana[J]. The plant cell, 2005, 17(12):3451-3469. doi: 10.1105/tpc.105.037036 URL
[8]	OLIVEIRA M C, JHALA A J, GAINES T, et al. Confirmation and control of HPPD-inhibiting herbicide-resistant waterhemp (Amaranthus tuberculatus) in Nebraska[J]. Weed technology, 2017, 31(1):67-79. doi: 10.1017/wet.2016.4 URL
[9]	邓希乐, 邓亚男, 吴昌炅. 天然产物作为除草剂安全剂的研究进展[J]. 农药学学报, 2023, 25(3):1-12.
[10]	农药室内生物测定试验准则除草剂第8部分:作物安全性试验茎叶喷雾法:NY/T 1155.8—2007[S]. 北京: 中国农业出版社, 2006.
[11]	张金艳. 除草剂安全剂3-二氯乙酰基-2,5-二甲基-2-乙基-1,3-噁唑烷对丁草胺解毒效果研究[J]. 黑龙江八一农垦大学学报, 2004, 16(3):78-81.
[12]	吴进才, 许俊峰, 冯绪猛, 等. 稻田常用农药对水稻3个品种生理生化的影响[J]. 中国农业科学, 2003, 36(5):536-541.
[13]	HABIG W H, PABST M J, JAKOBY W B. Glutathione S-transferases: The first enzymatic step in mercapturic acid formation[J]. Journal of biological chemistry, 1974, 249(22):7130-7139. doi: 10.1016/S0021-9258(19)42083-8 URL
[14]	BRADFORD M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J]. Analytical biochemistry, 1976, 72(1-2):248-254. doi: 10.1016/0003-2697(76)90527-3 URL
[15]	郭玉莲, 陶波, 高希武. 玉米谷胱甘肽转移酶(GSTs)特性及除草剂的诱导作用[J]. 玉米科学, 2008, 16(1):122-125.
[16]	DHOUIB I, JALLOULI M, ANNABI A, et al. From immunotoxicity to carcinogenicity: The effects of carbamate pesticides on the immune system[J]. Environmental science and pollution research, 2016, 23:9448-9458. doi: 10.1007/s11356-016-6418-6 URL
[17]	GIANNAKOPOULOS G, DITTGEN J, SCHULTE W, et al. Safening activity and metabolism of the safener cyprosulfamide in maize and wheat[J]. Pest management science, 2020, 76(10):3413-3422. doi: 10.1002/ps.5801 pmid: 32083366
[18]	CASIDA J E. Pesticide detox by design[J]. Journal of agricultural and food chemistry, 2018, 66(36):9379-9383. doi: 10.1021/acs.jafc.8b02449 pmid: 30113841
[19]	DENG X, ZHENG W, JIN C, et al. Novel phenylpyrimidine derivatives containing a hydrazone moiety protect rice seedlings from injury by metolachlor[J]. Bioorganic chemistry, 2021, 108:104645. doi: 10.1016/j.bioorg.2021.104645 URL
[20]	ANDREW P, JESSICA K. Herbicides[M]. Rijeka: IntechOpen, 2013:589-620.
[21]	SIVEY J D, LEHMLER H J, SALICE C J, et al. Environmental fate and effects of dichloroacetamide herbicide safeners: “Inert” yet biologically active agrochemical ingredients[J]. Environmental science & technology letters, 2015, 2(10):260-269.
[22]	叶非, 付颖, 徐伟钧, 等. 安全剂AD-67减轻单嘧磺隆对高粱药害的作用[J]. 植物保护, 2010, 36(2):105-108.
[23]	叶非, 付颖, 徐伟钧, 等. 安全剂AD-67保护玉米免受单嘧磺隆药害的作用及其机理[J]. 植物保护, 2010, 36(4):90-93.
[24]	ZHAO Y N, YE F, FU Y. Research progress on the action mechanism of herbicide safeners: A review[J]. Journal of agricultural and food chemistry, 2023.
[25]	BRAZIER-HICKS M, HOWELL A, COHN J, et al. Chemically induced herbicide tolerance in rice by the safener metcamifen is associated with a phased stress response[J]. Journal of experimental botany, 2020, 71(1):411-421. doi: 10.1093/jxb/erz438 URL
[26]	NANDULA V K, RIECHERS D E, FERHATOGLU Y, et al. Herbicide metabolism: Crop selectivity, bioactivation, weed resistance, and regulation[J]. Weed science, 2019, 67(2):149-175. doi: 10.1017/wsc.2018.88 URL
[27]	BAEK Y S, GOODRICH L V, BROWN P J, et al. Transcriptome profiling and genome-wide association studies reveal GSTs and other defense genes involved in multiple signaling pathways induced by herbicide safener in grain sorghum[J]. Frontiers in plant science, 2019, 10:192. doi: 10.3389/fpls.2019.00192 URL
[28]	CASTRO E, PUCCI C, DUARTE S, et al. Improved herbicide selectivity in tomato by safening action of benoxacor and fenclorim[J]. Weed technology, 2020, 34(5):647-651. doi: 10.1017/wet.2020.30 URL
[29]	DENG X. Current advances in the action mechanisms of safeners[J]. Agronomy, 2022, 12(11):2824. doi: 10.3390/agronomy12112824 URL
[30]	ZHANG Y Y, GAO S, HOANG M T, et al. Protective efficacy of phenoxyacetyl oxazolidine derivatives as safeners against nicosulfuron toxicity in maize[J]. Pest management science, 2021, 77(1):177-183. doi: 10.1002/ps.v77.1 URL
[31]	孙润红, 徐俊蕾, 杨丽荣, 等. 安全剂双苯恶唑酸对水稻精恶唑禾草灵药害的解毒效应[J]. 河南农业科学, 2017, 46(9):67-72.
[32]	SCARPONI L, QUAGLIARINI E, DEL BUONO D. Induction of wheat and maize glutathione S-transferase by some herbicide safeners and their effect on enzyme activity against butachlor and terbuthylazine[J]. Pest management science, 2006, 62(10):927-932. doi: 10.1002/ps.1258 pmid: 16835885
[33]	REQUEJO R, TENA M. Influence of glutathione chemical effectors in the response of maize to arsenic exposure[J]. Journal of plant physiology, 2012, 169(7):649-656. doi: 10.1016/j.jplph.2012.01.016 pmid: 22418430
[34]	BARTUCCA M L, CELLETTI S, ASTOLFI S, et al. Effect of three safeners on sulfur assimilation and iron deficiency response in barley (Hordeum vulgare) plants[J]. Pest management science, 2017, 73(1):240-245. doi: 10.1002/ps.4291 pmid: 27061021
[35]	WEI D, CHENG Z, BO T A O. Effect of chlorimuron-ethyl on biochemical mechanism in tolerant sugar beet[J]. Agricultural sciences in China, 2010, 9(12):1771-1776. doi: 10.1016/S1671-2927(09)60275-3 URL
[36]	YE F, ZHAI Y, GUO K L, et al. Safeners improve maize tolerance under herbicide toxicity stress by increasing the activity of enzymes in vivo[J]. Journal of agricultural and food chemistry, 2019, 67(42):11568-11576. doi: 10.1021/acs.jafc.9b03587 URL
[37]	YE F, ZHAI Y, KANG T, et al. Rational design, synthesis and structure-activity relationship of novel substituted oxazole isoxazole carboxamides as herbicide safener[J]. Pesticide biochemistry and physiology, 2019, 157:60-68. doi: S0048-3575(18)30531-5 pmid: 31153478
[38]	DERIDDER B P, DIXON D P, BEUSSMAN D J, et al. Induction of glutathione S-transferases in Arabidopsis by herbicide safeners[J]. Plant physiology, 2002, 130(3):1497-1505. doi: 10.1104/pp.010066 pmid: 12428014

处理名称	株高抑制率/%	株高保护率/%	鲜重抑制率/%	鲜重保护率/%
处理1	30.6±3.3 a	-	66.5±5.2 a	-
处理2	18.6±2.9 b	117.3±4.2 d	19.3±3.1 e	240.9±3.9 c
处理3	7.4±1.5 e	133.4±2.2 a	9.9±1.8 g	269.0±2.6 a
处理4	9.0±2.4 de	131.1±3.5 ab	15.6±3.2 f	251.9±4.4 b
处理5	19.1±2.5 b	116.6±3.6 d	47.8±2.9 b	155.8±3.2 f
处理6	11.6±0.9 cd	127.4±1.4 bc	35.9±1.5 c	191.3±2.2 e
处理7	12.8±2.6 c	125.6±3.8 c	30.9±3.1 d	206.3±4.1 d

处理名称	株高抑制率/%	株高保护率/%	鲜重抑制率/%	鲜重保护率/%
处理1	30.6±3.3 a	-	66.5±5.2 a	-
处理2	18.6±2.9 b	117.3±4.2 d	19.3±3.1 e	240.9±3.9 c
处理3	7.4±1.5 e	133.4±2.2 a	9.9±1.8 g	269.0±2.6 a
处理4	9.0±2.4 de	131.1±3.5 ab	15.6±3.2 f	251.9±4.4 b
处理5	19.1±2.5 b	116.6±3.6 d	47.8±2.9 b	155.8±3.2 f
处理6	11.6±0.9 cd	127.4±1.4 bc	35.9±1.5 c	191.3±2.2 e
处理7	12.8±2.6 c	125.6±3.8 c	30.9±3.1 d	206.3±4.1 d

安全剂环丙磺酰胺和双苯噁唑酸对玉米苯唑氟草酮药害的解毒作用

The Detoxification Effect of Safeners of Cyclopropanesulfonamide and Isoxadifen-ethyl against Fenpyrazone in Corn

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 3

参考文献 38

相关文章 15

编辑推荐

Metrics

本文评价

关于本刊

作者中心

审者中心

在线期刊

联系我们

[1]	杨苔, 于慧颖, 卢立明, 付海燕, 李国良, 代文龙, 刘春光, 杨峰山, 马玉堃. 促生潜力玉米种子内生细菌分离鉴定及发酵条件优化[J]. 中国农学通报, 2023, 39(9): 16-23.
[2]	钱双宏, 蔡世昆, 朱汉勇, 王绍斌, 李正润, 王应梅, 李绍云. 不同玉米品种（系）在云南省不同生态区丰产、稳产及适应性分析[J]. 中国农学通报, 2023, 39(9): 7-15.
[3]	杨海峰, 洪德峰, 马俊峰, 王群, 卫晓轶. DTOPSIS法在分析不同基因型玉米间混作效应中的应用[J]. 中国农学通报, 2023, 39(6): 13-17.
[4]	张可心, 鱼潇, 张谋草. 播期对陇东春玉米生长发育进程及产量的影响[J]. 中国农学通报, 2023, 39(6): 6-12.
[5]	于冲, 张凤云, 黄浩, 路运才. 玉米F₂群体轴部相关性状的数量遗传分析[J]. 中国农学通报, 2023, 39(5): 13-20.
[6]	黄浩, 路运才. 外源硅对低温胁迫下玉米和大刍草幼苗的影响[J]. 中国农学通报, 2023, 39(5): 21-27.
[7]	孟战赢, 蔡海峡, 杨浩哲, 王跃卿, 郭党, 李丽峰. 豫西地区青贮玉米光合和产量方面品种适应性研究[J]. 中国农学通报, 2023, 39(5): 28-32.
[8]	付瑜华, 蒙秋伊, 尚昆, 李秀诗, 刘凡值, 李祥栋. 玉米和高粱SSR标记在薏苡中的通用性研究[J]. 中国农学通报, 2023, 39(5): 33-38.
[9]	杨华, 蒋菊芳, 丁文魁, 程倩, 张金丹, 徐玉凤. 河西地区高温对春玉米生长的影响[J]. 中国农学通报, 2023, 39(3): 1-10.
[10]	杜明, 李潜龙, 康云海, 郑国利, 方玉. 饲用玉米的研究进展[J]. 中国农学通报, 2023, 39(24): 18-23.
[11]	赵凯丽, 李权辉, 刘瑜, 李萍, 陈娟, 颜芳, 闫实, 郭宁. 北京郊区不同肥力水平下玉米的施氮量推荐[J]. 中国农学通报, 2023, 39(24): 24-30.
[12]	提俊阳, 张玉芹, 杨恒山, 张瑞富, 邰继承, 萨如拉, 韩镁琪. 豆米轮作对春玉米土壤固氮细菌群落结构及多样性的影响[J]. 中国农学通报, 2023, 39(2): 44-50.
[13]	关婷, 樊明寿, 贾立国. 基于微型蒸发器估算不同作物棵间蒸发的研究进展[J]. 中国农学通报, 2023, 39(2): 63-67.
[14]	肖本泽, 王资霖. 转EPSPS基因抗除草剂水稻恢复系的培育及育种评价[J]. 中国农学通报, 2023, 39(2): 8-15.
[15]	郭新东, 孙瑜, 张永梅. 近红外叶片水分无损检测系统设计与试验[J]. 中国农学通报, 2023, 39(19): 153-158.