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中国农学通报

中国农学通报 ›› 2024, Vol. 40 ›› Issue (35): 130-137.doi: 10.11924/j.issn.1000-6850.casb2024-0262

• 食品·营养·检测·安全 • 上一篇    下一篇

破壁灵芝孢子粉多糖抗氧化活性及稳定性研究

毛雪(), 殷博, 张欣, 刘煜, 辛丽欣, 孔祥辉(), 王佳龙()   

  1. 黑龙江省科学院微生物研究所，哈尔滨 150010
  • 收稿日期:2024-04-18 修回日期:2024-06-15 出版日期:2024-12-15 发布日期:2024-12-12
  • 通讯作者:
    孔祥辉，女，1971年出生，黑龙江哈尔滨人，研究员，博士，研究方向：食药用菌精深加工。通信地址：150010 黑龙江哈尔滨道里区兆麟街68号 黑龙江省科学院微生物研究所，Tel：0451-84614297，E-mail：；
    王佳龙，男，1977年出生，黑龙江哈尔滨人，副研究员，学士，研究方向：微生物学。通信地址：150010 黑龙江哈尔滨道里区兆麟街68号 黑龙江省科学院微生物研究所，Tel：0451-84614297，E-mail：。
  • 作者简介:

    毛雪，女，1990年出生，黑龙江哈尔滨人，助理研究员，硕士，研究方向：食药用菌精深加工。通信地址：150010 黑龙江哈尔滨道里区兆麟街68号 黑龙江省科学院微生物研究所，Tel：0451-84614297，E-mail：。

  • 基金资助:
    2023省财政厅-省属科研院所业务费项目“灵芝孢子粉多糖和三萜制备关键技术研究”(2023SSKY004-02)

Antioxidant Activity and Stability of Polysaccharides from Ganoderma lucidum Spore Powder

MAO Xue(), YIN Bo, ZHANG Xin, LIU Yu, XIN Lixin, KONG Xianghui(), WANG Jialong()   

  1. Institute of Microbiology, Heilongjiang Academy of Sciences, Harbin150010
  • Received:2024-04-18 Revised:2024-06-15 Published:2024-12-15 Online:2024-12-12

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摘要：

本研究旨在探讨不同提取方法对破壁灵芝孢子粉多糖抗氧化活性与稳定性的影响，为产品商业化生产及稳定性优化提供科学依据。采用4种方法提取破壁灵芝孢子粉多糖（热水浸提法(LPH)、超声辅助法(LPU)、微波辅助法(LPM)、酶解辅助法(LPE)），测定多糖得率和抗氧化能力，并通过模拟食品加工条件，系统评估多糖的氧化稳定性。结果显示，LPE的灵芝孢子粉多糖得率和糖醛酸含量最高，分别为5.05%和6.55%。LPE的抗氧化能力最强，DPPH自由基最高清除率为77.24%，羟自由基最高清除率为83.04%。光照对LPE的抗氧化稳定性影响较大，在酸性条件下其抗氧化活性较好，羟自由基清除率超过85%，且表现出良好的耐热性。此外，甜味剂和碱性添加剂对LPE的抗氧化稳定性影响显著，产品灭菌可优先选择巴氏杀菌。研究发现，与另外3种提取方法相比，酶解法具有作用条件温和、提取率高、可制备特定结构的多糖、减少杂质提高多糖纯度以及能够在相对较短时间内实现大量多糖提取等优点，能够满足产业化需求。LPE的抗氧化活性最强，具有较好的耐热稳定性，但在灵芝孢子粉多糖产品的加工贮藏过程中，应避免长时间光照，控制甜味剂和碱性添加剂的使用剂量，确保产品的质量。

关键词: 破壁灵芝孢子粉, 多糖提取, 含量, 抗氧化, 加工条件, 稳定性

Abstract:

This study aims to explore the impact of different extraction methods on the antioxidant activity and stability of polysaccharides from broken Ganoderma lucidum spore powder, and provide a scientific basis for commercial production and stability optimization of products. Polysaccharides were prepared by hot water extraction, ultrasound-assisted extraction, microwave-assisted extraction, and enzymatic hydrolysis-assisted extraction. The polysaccharide yield and antioxidant capacity were determined. Through simulating food processing conditions, the oxidation stability of polysaccharides was systematically evaluated. The polysaccharides extracted from broken Ganoderma lucidum spore powder by enzymatic hydrolysis-assisted method (LPE) had the highest yield and uronic acid content, which were 5.05% and 6.55% respectively. LPE had the strongest antioxidant capacity. The highest scavenging rate of DPPH free radicals was 77.24%, and the highest scavenging rate of hydroxyl radicals was 83.04%. Light exposure had a great influence on the antioxidant stability of LPE. Under acidic conditions, it had better antioxidant activity. The scavenging rate of hydroxyl radicals exceeded 85%. Moreover, LPE showed good heat resistance. In addition, sweeteners and alkaline additives had a significant impact on the antioxidant stability of LPE. For product sterilization, high-pressure sterilization can be preferred. LPE had the strongest antioxidant activity. During the processing and storage of Ganoderma lucidum spore powder polysaccharide products, prolonged light exposure should be avoided, and the dosage of sweeteners and alkaline additives should be controlled to ensure product quality.

Key words: broken Ganoderma lucidum spore powder, polysaccharide extraction, content, antioxidant, processing conditions, stability