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中国农学通报

中国农学通报 ›› 2025, Vol. 41 ›› Issue (12): 142-148.doi: 10.11924/j.issn.1000-6850.casb2024-0370

• 食品·营养·检测·安全 • 上一篇    下一篇

藜麦苗总黄酮提取优化及其对黄嘌呤氧化酶的抑制作用和抗氧化活性评价

张述伟(), 黄琳丽, 宗营杰, 郭慧敏, 郭桂梅, 杨邦伟, 陈志伟, 徐红卫, 刘成洪()   

  1. 上海市农业科学院生物技术研究所/上海市农业遗传育种重点实验室，上海 201106
  • 收稿日期:2024-05-07 修回日期:2024-09-15 出版日期:2025-04-25 发布日期:2025-04-24
  • 通讯作者:
    刘成洪，男，1975年出生，湖北宜昌人，研究员，博士，研究方向：麦类作物遗传育种及功能开发研究。通信地址：201106 上海市闵行区北翟路2901号，上海市农业科学院，Tel：021-62202871，E-mail： 。
  • 作者简介:

    张述伟，男，1994年出生，安徽宿州人，助理实验师，学士，研究方向：作物功能成分分析与评价。通信地址：201106 上海市闵行区北翟路2901号，上海市农业科学院，Tel：021-62202871，E-mail：。

  • 基金资助:
    上海市科技兴农项目“功能藜麦中活性黄酮的鉴定及其品质评价”(2022-02-08-00-12-F01171); 上海市农业科学院卓越团队建设计划“作物小孢子高效育种技术及功能研发”(沪农科卓(2022)018); 上海市科委国内合作项目“云南藜麦高值化利用关键技术及示范应用”(22015810100)

Optimization of Total Flavonoids Extraction from Quinoa Seedlings and Evaluation of Its Inhibitory Effect on Xanthine Oxidase and Antioxidant Activity

ZHANG Shuwei(), HUANG Linli, ZONG Yingjie, GUO Huimin, GUO Guimei, YANG Bangwei, CHEN Zhiwei, XU Hongwei, LIU Chenghong()   

  1. Shanghai Key Laboratory of Agricultural Genetics and Breeding/Biotech Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106
  • Received:2024-05-07 Revised:2024-09-15 Published:2025-04-25 Online:2025-04-24

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摘要：

为探究藜麦苗的总黄酮提取方法、其在生长过程中含量变化、抗氧化活性、以及对黄嘌呤氧化酶（XOD）的抑制作用。以不同生长时期的藜麦苗为试验材料，采用单因素试验结合响应面法对超声波提取藜麦苗总黄酮的方法进行优化。其最优提取条件为：乙醇体积分数60%，料液比1:34，温度20℃，超声功率300 W，提取时间24 min；生长42 d时藜麦苗中总黄酮含量最高，为26.55 mg/g；抗氧化活性在28 d时最高，羟自由基清除能力、ABTS自由基清除能力和DPPH自由基清除能力分别为9.76%、60.50 mg/g、16.28 mg/g；藜麦苗总提取物对XOD的抑制作用随着浓度升高而逐渐增强，半抑制浓度（IC50值）为5.27 mg/mL。本研究为抗氧化和预防高尿酸血症的藜麦苗新产品的开发和功能成分研究提供提取方法和参考依据。

关键词: 藜麦苗, 总黄酮, 响应面, 抗氧化活性, 黄嘌呤氧化酶

Abstract:

To investigate the extraction method of total flavonoids from quinoa seedlings, the change of its content during growth, as well as its antioxidant activity and inhibition effect on XOD, the total flavonoids from quinoa seedlings at different growth stages were used as test materials. The total flavonoids of quinoa seedlings were extracted by single factor test combined with response surface optimization ultrasonic method. The optimal extraction conditions were as follows: ethanol volume fraction of 60%, solid-liquid ratio of 1:34, temperature of 20℃, ultrasonic power of 300 W, extraction time of 24 min. The total flavonoid content of quinoa seedlings was the highest at 42 days of growth, which was 26.55 mg/g. The antioxidant activity was the highest at 28 days, and the scavenging capacity of hydroxyl free radical, ABTS free radical and DPPH free radical were 9.76%, 60.50 mg/g and 16.28 mg/g, respectively. The inhibitory effect of the total extract of quinoa seedlings on XOD gradually increased with increasing concentration, with a half-inhibitory concentration (IC50 value) of 5.27 mg/mL. This study provided the extraction method and reference for the development and functional component research of new quinoa seedling products for antioxidation and hyperuricemia prevention.

Key words: quinoa seedlings, total flavonoids, response surface, antioxidant activity, xanthine oxidase