2种AMF介导下紫苏根际土壤理化性质和微生物多样性差异

doi:10.11924/j.issn.1000-6850.casb2024-0527

中国农学通报 ›› 2025, Vol. 41 ›› Issue (28): 102-110.doi: 10.11924/j.issn.1000-6850.casb2024-0527

• 资源·环境·生态·土壤·气象 • 上一篇下一篇

2种AMF介导下紫苏根际土壤理化性质和微生物多样性差异

刘雅琪(), 劳雅慧, 李冲伟()

黑龙江大学生命科学学院农业微生物技术教育部工程研究中心/黑龙江省寒地生态修复与资源利用重点实验室/黑龙江省普通高校微生物重点实验室，哈尔滨 150080

收稿日期:2024-08-11 修回日期:2024-12-24 出版日期:2025-10-05 发布日期:2025-10-10
通讯作者:
李冲伟，男，1977年出生，黑龙江佳木斯人，教授，博士，研究方向：微生物生态学。通信地址：150080 黑龙江省哈尔滨市南岗区黑龙江大学，E-mail：chongweili@126.com。
作者简介:
刘雅琪，女，1999年出生，河北保定人，硕士研究生，研究方向：微生物生态学。通信地址：150080 黑龙江省哈尔滨市南岗区黑龙江大学，E-mail：Yaky9962@163.com。
基金资助:
黑龙江省自然科学基金项目“寒区农业土壤面源污染微生物调控技术的研究”(TD2019C002)

Differences in Physicochemical Properties and Microbial Diversity of Perilla Rhizosphere Soil Mediated by Two Arbuscular Mycorrhizal Fungi

LIU Yaqi(), LAO Yahui, LI Chongwei()

Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education/Heilongjiang Provincial Key Laboratory of Ecological Restoration and Resource Utilization for Cold Region/Key Laboratory of Microbiology, College of Heilongjiang Province/ School of Life Sciences, Heilongjiang University, Harbin 150080

Received:2024-08-11 Revised:2024-12-24 Published:2025-10-05 Online:2025-10-10

摘要/Abstract

摘要：

连作障碍现象严重制约紫苏的产量和质量。为探究不同丛枝菌根真菌(AMF)对紫苏连作障碍的缓解效果，通过向连作土壤中分别添加丛枝菌根真菌(AMF)根内根孢囊霉(RI)和摩西管柄囊霉(FM)，测定紫苏地上生长指标，结合土壤理化性质、土壤酶活性及微生物群落结构，探讨2种AMF对紫苏生长的影响。结果表明，添加2种AMF能显著提高土壤中有机质、全氮、全磷和全钾含量(P<0.05)，提高土壤脲酶和磷酸酶活性，增加土壤微生物群落中有益菌丰度，连作土壤酸化趋势得到缓解，紫苏生长指标明显提高。2种AMF相比，RI在提高土壤酶活性方面影响更加明显，而FM改善土壤微生物群落结构的能力更强。研究阐明了2种AMF改善连作紫苏土壤的功能差异，为下一步筛选缓解紫苏连作障碍的微生物菌剂提供了理论依据。

关键词: 紫苏, 连作障碍, 理化性质, 微生物, 丛枝菌根真菌, 根内根孢囊霉, 摩西管柄囊霉

Abstract:

Continuous cropping obstacles have significantly impacted the yield and quality of Perilla. To explore the mitigation effects of microbial agents on Perilla’s continuous cropping obstacles, two different arbuscular mycorrhizal fungi (AMF), which were Rhizophagus intraradices (RI) and Funneliformis mosseae (FM), were added to the continuous cropping soil. By measuring Perilla’s growth indicators, the physicochemical properties of rhizosphere soil, soil enzyme activity and microbial community structure, the differential effects of the two AMFs on Perilla growth were investigated. The results showed that the addition of AMF significantly increased the content of organic matter, total nitrogen, total phosphorus, and total potassium in the soil (P<0.05), enhanced soil urease and phosphatase activity, and increased the abundance of beneficial bacteria in the soil microbial community structure. The addition of AMF alleviated soil acidification and improved the growth status of Perilla. Compared to FM, RI had a more significant effect on Perilla growth and soil enzyme activity, while FM had a stronger ability to improve soil microbial community structure. This study elucidated the functional differences between the two AMFs in alleviating the continuous cropping obstacles of Perilla, provided a theoretical basis for the screening of microbial agents to mitigate these obstacles in future research.

Key words: Perilla, continuous cropping obstacles, physical and chemical properties, microorganisms, arbuscular mycorrhizal fungi, Rhizophagus intraradices (RI), Funneliformis mosseae (FM)

刘雅琪, 劳雅慧, 李冲伟. 2种AMF介导下紫苏根际土壤理化性质和微生物多样性差异[J]. 中国农学通报, 2025, 41(28): 102-110.

LIU Yaqi, LAO Yahui, LI Chongwei. Differences in Physicochemical Properties and Microbial Diversity of Perilla Rhizosphere Soil Mediated by Two Arbuscular Mycorrhizal Fungi[J]. Chinese Agricultural Science Bulletin, 2025, 41(28): 102-110.

图/表 9

参考文献 46

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指标	CK	RI	FM
pH	6.54±0.11b	6.86±0.06a	6.83±0.10a
铵态氮/(mg/kg)	30.16±2.35a	22.83±1.63b	21.17±1.58b
速效磷/(mg/kg)	47.63±3.76b	60.46±4.17a	56.06±4.39a
速效钾/(mg/kg)	64.33±5.59c	100.67±4.51a	90.45±4.98b
有机质/(g/kg)	39.82±2.85c	45.24±3.57b	52.87±3.12a
全氮/(g/kg)	1.50±0.04b	1.64±0.07a	1.63±0.06a
全磷/(g/kg)	0.88±0.02b	0.92±0.02ab	0.92±0.01a
全钾/(g/kg)	20.8±0.51b	22.6±0.41a	21.56±0.46ab

指标	CK	RI	FM
pH	6.54±0.11b	6.86±0.06a	6.83±0.10a
铵态氮/(mg/kg)	30.16±2.35a	22.83±1.63b	21.17±1.58b
速效磷/(mg/kg)	47.63±3.76b	60.46±4.17a	56.06±4.39a
速效钾/(mg/kg)	64.33±5.59c	100.67±4.51a	90.45±4.98b
有机质/(g/kg)	39.82±2.85c	45.24±3.57b	52.87±3.12a
全氮/(g/kg)	1.50±0.04b	1.64±0.07a	1.63±0.06a
全磷/(g/kg)	0.88±0.02b	0.92±0.02ab	0.92±0.01a
全钾/(g/kg)	20.8±0.51b	22.6±0.41a	21.56±0.46ab

土壤酶	CK	RI	FM
脲酶	0.53±0.07b	0.68±0.06a	0.75±0.06a
蔗糖酶	6.56±0.50a	4.59±0.44b	3.58±0.17c
磷酸酶	1.54±0.12b	1.74±0.14a	1.72±0.08a
过氧化氢酶	0.45±0.06c	0.88±0.07a	0.72±0.04b
过氧化物酶	2.45±0.31b	3.12±0.47a	2.47±0.27b

土壤酶	CK	RI	FM
脲酶	0.53±0.07b	0.68±0.06a	0.75±0.06a
蔗糖酶	6.56±0.50a	4.59±0.44b	3.58±0.17c
磷酸酶	1.54±0.12b	1.74±0.14a	1.72±0.08a
过氧化氢酶	0.45±0.06c	0.88±0.07a	0.72±0.04b
过氧化物酶	2.45±0.31b	3.12±0.47a	2.47±0.27b

样本	Chao1	ACE	Shannon	Simpson
CK	3032.77±24.67b	3081.49±38.38b	6.09±0.02b	0.0067±0.0008b
RI	3122.39±61.53ab	3200.03±55.49ab	6.32±0.05a	0.0069±0.0011ab
FM	3161.58±64.61a	3254.64±78.68a	6.31±0.04a	0.0071±0.0004a

2种AMF介导下紫苏根际土壤理化性质和微生物多样性差异

Differences in Physicochemical Properties and Microbial Diversity of Perilla Rhizosphere Soil Mediated by Two Arbuscular Mycorrhizal Fungi

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样本	Chao1	ACE	Shannon	Simpson
CK	424.69±39.79b	419.19±41.55b	3.93±0.08c	0.0409±0.0053b
RI	507.73±50.35a	504.55±46.33a	4.31±0.17a	0.0642±0.0389a
FM	519.16±54.08a	518.48±52.74a	4.09±0.07b	0.0466±0.0032b

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