欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2025, Vol. 41 ›› Issue (35): 1-8.doi: 10.11924/j.issn.1000-6850.casb2025-0762

• 农学·农业基础科学 •    下一篇

微生物菌剂与海藻肥联合施用对烟叶品质及叶际微生物的影响

陈黎1(), 范才银2, 苏亮3, 胡亚军4, 张德咏5,6, 朱春晖6,7, 封卫平1, 周劲东1, 程菊娥6,7, 杜晓华6,7, 孙书娥6,7()   

  1. 1 湖南省烟草公司衡阳市公司，湖南衡阳 421000
    2 湖南省烟草公司衡阳市公司常宁市分公司，湖南常宁 421500
    3 吉林烟草工业有限责任公司，吉林延吉 133000
    4 湖南农业大学农学院，长沙 410128
    5 湖南省农业科学院，长沙 410125
    6 岳麓山实验室，长沙 410128
    7 湖南省农业科学院植物保护研究所，长沙 410125
  • 收稿日期:2025-09-08 修回日期:2025-11-17 出版日期:2025-12-11 发布日期:2025-12-11
  • 通讯作者:
    孙书娥，女，1982年出生，山东莘县人，助理研究员，博士，主要从事植物与微生物互作机制研究。通信地址：410128 湖南省长沙市芙蓉区东湖街道红旗路246号 湖南省农科院片区15号楼，E-mail：。
  • 作者简介:

    陈黎，男，1983年出生，湖南衡阳人，助理农艺师，硕士，研究方向：烟草栽培及调制。通信地址：421000 湖南省衡阳市华新开发区延安路8号 湖南省烟草公司衡阳市公司，E-mail：。

  • 基金资助:
    湖南省烟草公司衡阳市公司项目“烟稻轮作生产力与土壤健康协同提升技术研究与示范”(HYYC2023KJ28); 湖南省农业科技创新资金项目“水稻微生物组组装关键调控基因Os4CL1的作用机制与抗稻瘟病机制”(2024CX36)

Effects of Combined Application of Microbial Agents and Seaweed Fertilizer on Tobacco Leaf Quality and Phyllosphere Microorganisms

CHEN Li1(), FAN Caiyin2, SU Liang3, HU Yajun4, ZHANG Deyong5,6, ZHU Chunhui6,7, FENG Weiping1, ZHOU Jindong1, CHENG Ju’e6,7, DU Xiaohua6,7, SUN Shu’e6,7()   

  1. 1 Hengyang Branch of Hunan Tobacco Company, Hengyang, Hunan 421000
    2 Changning Branch of Hengyang Tobacco Company, Hunan Tobacco Company, Changning, Hunan 421500
    3 Jilin Tobacco Industry Co., Ltd. Yanji, Jilin 133000
    4 College of Agronomy, Hunan Agricultural University, Changsha 410128
    5 Hunan Academy of Agricultural Sciences, Changsha 410125
    6 Yuelushan Laboratory, Changsha 410128
    7 Hunan Institute of Plant Protection, Hunan Academy of Agricultural Sciences, Changsha 410125
  • Received:2025-09-08 Revised:2025-11-17 Published:2025-12-11 Online:2025-12-11

RichHTML

1

PDF (PC)

22

摘要：

本研究旨在探究联合施用沼泽红假单胞菌、贝莱斯芽孢杆菌与海藻有机水溶肥对烟叶品质及叶际微生态的影响。采用田间小区试验，设置处理1(Rho)：5×109 cfu/mL沼泽红假单胞菌GJ-22 300倍液；处理2(Rhor)：5×109 cfu/mL沼泽红假单胞菌GJ-22 300倍液和150 g/L海藻有机水溶肥1000倍液；处理3(Bav)：5×109 cfu/mL贝莱斯芽孢杆菌300倍液；处理4(Baor)：5×109 cfu/mL贝莱斯芽孢杆菌300倍液和150 g/L海藻有机水溶肥1000倍液；处理5(ORF)：150 g/L海藻有机水溶肥1000倍液；处理6(CK)：空白对照，共6个处理。测定烟草的农艺性状、主要化学成分，分析叶际微生物群落结构变化。结果表明：处理2显著促进烤烟生长，提高了烤烟C3F和C4F等级烟叶的比例，从而显著提高了烟叶品质；处理1显著提高了烟叶总糖含量。在叶际微生物方面，处理2和处理4均显著增加了细菌中γ变形菌纲的相对丰度，同时降低了拟杆菌纲的相对丰度。处理1对叶际细菌和真菌的多样性具有增加趋势，但与有机水溶肥联合应用后，对细菌和真菌的多样性影响不显著。处理3对细菌多样性影响不显著，但显著降低了叶际真菌的多样性，与处理5相比，显著降低了叶际细菌的多样性。这些研究结果可为生产中微生物菌剂和有机水溶肥在烟草生产中的应用推广提供理论依据。

关键词: 沼泽红假单胞菌, 贝莱斯芽孢杆菌, 海藻有机肥, 烟叶品质, 叶际微生物

Abstract:

To study the effects of combined application of Rhodopseudomonas palustris, Bacillus velezensis, and seaweed-derived organic water-soluble fertilizer on tobacco leaf quality and phyllosphere microbial ecology, a field plot experiment was conducted with 6 treatments: 5×109 cfu/mL Rhodopseudomonas palustris GJ-22 diluted 300× (treatment 1, Rho); 5×109 cfu/mL R. palustris GJ-22 (300×)+150 g/L seaweed organic water-soluble fertilizer (1000×) (treatment 2, Rhor); 5×109 cfu/mL B. velezensis (300×) (treatment 3, Bav); 5×109 cfu/mL B. velezensis (300×)+150 g/L seaweed fertilizer (1000×) (treatment 4, Baor); 150 g/L seaweed fertilizer (1000×) (treatment 5, ORF); blank control (treatment 6, CK). Agronomic traits and main chemical components of tobacco were measured, and changes in the phyllosphere microbial community structure were analyzed. The results showed that treatment 2 significantly promoted the growth of flue-cured tobacco, increased the relative proportion of C3F and C4F grades, and markedly improved the leaf quality; treatment 1 significantly increased total sugar content in leaves; treatment 2 and 4 significantly increased the relative abundance of Gammaproteobacteria while reducing that of Bacteroidia in the phyllosphere; treatment 1 tended to increase the bacterial and fungal diversity in the phyllosphere, but its combination with organic water-soluble fertilizer did not significantly affect microbial diversity; treatment 3 had no significant effect on the bacterial diversity but significantly reduced fungal diversity; compared with treatment 5, it also significantly reduced the bacterial diversity in the phyllosphere. These findings provide a theoretical basis for the field application of microbial agents and organic water-soluble fertilizers.

Key words: Rhodopseudomonas palustris, Bacillus velezensis, seaweed organic fertilizer, tobacco quality, phyllosphere microorganisms