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中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (16): 181-183.

• 林学 园艺 园林 • 上一篇    下一篇

波斯顿蕨离体培养技术的研究

司 亮,尤海波   

  • 收稿日期:2009-04-20 修回日期:2009-05-19 出版日期:2009-08-20 发布日期:2009-08-20

Studies on method of culturation in vitro of Nephrolepis exaltata

Si Liang, You Haibo   

  • Received:2009-04-20 Revised:2009-05-19 Online:2009-08-20 Published:2009-08-20

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摘要： 以波斯顿蕨走茎茎尖为外植体研究无菌培养的繁殖方法，结果表明，最佳诱导培养基为MS+BA0.5,增殖培养基为MS+BA0.7,增殖系数为7.2；孢子体分化培养基为MS+BA1.0+NAA0.5，诱导率可达到92% ；适宜的生根培养基为1/2MS+BA1.0+IBA0.2+0.1% 活性炭,生根率为100%。

关键词: 超氧化物歧化酶, 超氧化物歧化酶, 过氧化氢酶, 脂质过氧化, 水分胁迫, 半同胞家系, 湿地松

Abstract: using the stem apex of the stolon of Nephrolepis exaltata as explants ,It shows that the most appropriate induction culture medium is MS +BA0.5mg/L,the ratio of induction is 90%.The most appropriate secondry culture medium is MS+BA0.7mg/L，multiplication coefficient was to be 7.2；The most appropriate roots-planting culture medium is 1/2MS + IBA 0.2mg/L + 1.0% Ac,and the rooting rate is up to 100%.