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中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (8): 38-41.

所属专题： 生物技术

• 生物技术科学 • 上一篇    下一篇

桃果实乙烯反应因子PpERF1全长基因的克隆及序列分析

赵相娟,张 静,魏绍冲   

  • 收稿日期:2008-11-24 修回日期:2008-12-19 出版日期:2009-04-20 发布日期:2009-04-20

Cloning and Sequencing Analysis of PpERF1 Gene from Peach Fruit

  • Received:2008-11-24 Revised:2008-12-19 Online:2009-04-20 Published:2009-04-20

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摘要： 以成熟肥城桃果实的cDNA为模板，根据EST库中的核苷酸序列，设计2条特异引物分别与B26进行PCR扩增，结果得到了该基因下游包括3′ 端非编码区的765 bp大小的cDNA片段。PpERF1基因全长为1 263 bp，包含一个708 bp大小的完整开放阅读框(ORF)。序列分析表明，PpERF1与番茄、拟南芥、苹果和葡萄等在DNA结合功能域的约58个氨基酸中，同源性可达68.4%-100%，且在该区域中存在1个α-螺旋和3个反平行链构成的β-折叠结构。经同源性分析，该序列与拟南芥、葡萄、豌豆等植物的氨基酸同源性为58.8%～67.5%，属于同一类ERF家族成员。

关键词: 旱作, 旱作, 水作, 加工品质, 影响

Abstract: According to the nucleic acid sequence from EST database, two specific primers were designed and a 765 bp cDNA fragment containing 3′ untranslated region was amplified by RT-PCR from ripening peach fruit. The cDNA, named PpERF1, contains an ORF (open reading frame) which is 708 bp in length. PpERF1 contains a highly conserved, plant-specific DNA-binding domain, which is composed of three β-sheets and an α-helix. Multiple alignment of the ERF conserved domains shows that PpERF1 has 68.4%-100% identity to ERF homolog in other plants. PpERF1 shared high similarity at both nucleotide and polypeptide level with corresponding sequences from Arabidopsis, grape and pea.