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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (12): 277-281.

所属专题： 生物技术

• 植物保护 农药 • 上一篇    下一篇

核盘菌对腐霉利生物学及生理生化特性抗药性研究

刘永红1,刘慧平2,李新凤2,韩巨才2,刘慧芹3   

  • 收稿日期:2010-02-02 修回日期:2010-03-05 出版日期:2010-06-20 发布日期:2010-06-20
  • 基金资助:

    山西省留学归国基金

Biological, physiological and biochemical characteristics were studied on resistance of Sclerotinia sclerotiorum to procymidone

Liu Yonghong1, Liu Huiping2, Li Xinfeng2, Han Jucai2, Liu Huiqin3   

  • Received:2010-02-02 Revised:2010-03-05 Online:2010-06-20 Published:2010-06-20

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摘要：

本文研究了抗腐霉利的核盘菌突变抗性株与亲本敏感株的生物学及生理生化特性。结果表明：突变株与亲本株对碳、氮源的利用情况和对pH值的要求有一定差别：突变株分别以在乳糖为碳源和缺氮处理的培养基中以及pH值为6~7时生长最快，而亲本株在乳糖为碳源和蛋白胨为氮源的培养基上以及pH值为5时生长最快。二者在不同温度下生长情况基本相似。在相同的渗透压条件（葡萄糖浓度）下，突变株对渗透压较亲本株更为敏感。亲本株对低渗透压（1%）表现敏感，突变株对高渗透压（> 4%时）表现敏感。通过测定相对电导率，发现在相同处理时间内，突变株电导率均高于亲本株，并随着处理的延长，二者的电导率均升高。用不同浓度腐霉利处理或饥饿处理时，突变株与亲本株PAL活性均增加，但突变株PAL活性始终高于亲本株。表明突变株的抗药性与其渗透调节、电解质渗透和相关酶活性具有一定的相关性。

关键词: 水杨酸（SA）, 水杨酸（SA）, 草莓, 白粉病, 设施栽培

Abstract:

Biological, physiological and biochemical characteristics were studied between procymidone-resistant mutants and their parent sensitive strains of Sclerotinia sclerotiorum. The results showed the carbon-nitrogen source and pH value were different between the mutants and the parent strains. The mutants and parent strains grew fastest respectively when carbon-nitrogen source and pH value were lactose, non-nitrogen, 6-7 and lactose, peptone and 5. Both grew similar in different temperatures. On the same osmotic stress (dextrose concentration), the mutants had more sensitive to osmotic stress than parent strains. The parent strains and mutants respectively were sensitive to low (1%) and high (> 4%) osmotic stress. Relative conductivity of the mutants was always higher than that of parent strains in same treat time. Relative conductivity of both rose with extension of treat time. Respectively treated with hunger and procymidone in different concentrations, the activity of PAL (phenlalanine ammona-lyase) of both increased, but the mutant had higher PAL activity than its present strain. It indicated that resistance of the mutants was related to its osmotic regulation, electrolyte leakage and correlate enzyme activity.