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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (12): 35-39.

所属专题： 生物技术

• 生物技术科学 • 上一篇    下一篇

基因及构建特异性PCR方法检测转基因香石竹

贾军伟1,2,孙建萍2,白蓝3,李鹏2,潘爱虎2   

  • 收稿日期:2010-02-02 修回日期:2010-03-03 出版日期:2010-06-20 发布日期:2010-06-20

Gene and construct–specific qualitative PCR detection of transgenic carnations

Jia Junwei 1,2, Sun Jianping 2, Bai Lan3, Li Peng2, Pan Aihu2   

  • Received:2010-02-02 Revised:2010-03-03 Online:2010-06-20 Published:2010-06-20

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摘要：

摘要：以澳大利亚Florigene公司和日本Suntory公司研发的两种转基因香石竹Moonshade、Moonlite为研究对象，针对内参照基因ANS和外源基因F3’5’H、CHS启动子、D8ter，建立基因特异性定性PCR检测方法。此外，分别在外源MAC启动子和DFR基因上设计PCR引物，开展构建特异性PCR检测，定性PCR方法的检测灵敏度为0.5%。该方法的建立为转基因香石竹的进口检测、国内监管和环境安全评价提供了初步数据。

关键词: 硼磷配施, 硼磷配施, 甘蓝型油菜, 生长, 品质

Abstract:

Abstract: In this study, In view of the endogenous reference gene ANS and the exogenous genes such as F3'5'H, CHS promoter, D8ter, the gene and construct–specific qualitative PCR detection methods were established for transgenic carnations——Moonshade、Moonlite which were developed by Japanase Suntory company and Australian Florigene company. In addition, PCR primers were designed respectively in the exogenous MAC gene promoter and the DFR gene, construct–specific qualitative PCR detection was carried out. The detection sensitivity reached 0.50%. The establishment of this method provides preliminary data for the transgenic carnation import detection, domestic supervision and environmental safety assessment..