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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (18): 406-409.

• 农学 农业基础科学 • 上一篇    下一篇

广西北海红树林土壤放线菌BH0954的分离和鉴定

黄大林 袁桂峰 徐雅娟 骆耐香 陈建宏 刘菁 蒋莲秀 陈森洲   

  • 收稿日期:2010-03-29 修回日期:2010-05-17 出版日期:2011-09-20 发布日期:2011-09-20
  • 基金资助:
    广西教育厅科研资助项目

Beihai, Guangxi Mangrove soil actinomycetes BH0954 Isolation and identification of

  • Received:2010-03-29 Revised:2010-05-17 Online:2011-09-20 Published:2011-09-20

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摘要： 摘要：【目的】对具有抗菌活性红树林土壤放线菌的进行分离和鉴定。【方法】用海水配制高氏一号培养基，采用稀释分离法将所采集的土壤样本进行分离纯化，通过抗菌试验筛选，从广西北海红树林高盐泥样中分离到一株放线菌BH0954，该菌株的发酵产物具有很强的抗菌活性。该菌株在高氏一号培养基上生长良好，最适生长温度28 ℃，最适生长pH为7.0，观察其形态特征和生理生化特征；提取其基因组总DNA，用通用引物对其16s rDNA进行PCR扩增，扩增产物进行DNA序列测定。【结果】根据其形态学特征、生理生化特征和基于16S rDNA基因序列的系统发育分析结果，BH0954初步被鉴定为链霉菌属的有效发表种仙台链霉菌（Streptomyces sindenensis）的变种。【结论】红树林土壤放线菌BH0954具有抗菌活性，本文的研究结果为进一步开发利用广西红树林土壤放线菌资源奠定基础。

关键词: HIVs, HIVs, 释放机制, 应用

Abstract: Abstract: 【Objective】 antibacterial activity of mangrove soil actinomycetes of the separation and identification. 【Methods】 Gao on the 1st medium prepared with sea water used to dilute the separation of soil samples collected from isolated and purified by anti-bacterial screening tests, from Beihai, Guangxi Mangrove high-salt soil samples was isolated actinomycetes BH0954 The fermentation product of strain has a strong antimicrobial activity. The strain grew well on medium Gao on the 1st, and the optimum growth temperature 28 ℃, optimum growth pH of 7.0 to observe its morphological characteristics and physiological and biochemical characteristics; withdraw their total genomic DNA, using universal primers to their 16s rDNA PCR amplification, amplified products, DNA sequencing. 【Results】 based on their morphological characteristics, physiological and biochemical characteristics and 16S rDNA gene sequence-based phylogenetic analysis, BH0954 initially was identified as the genus Streptomyces Sendai kinds of effective publication of Streptomyces (Streptomyces sindenensis) variants. 【Conclusion】 mangrove soil actinomycetes BH0954 has antibacterial activity, this paper's findings for the further development and utilization of mangroves in Guangxi soil actinomycetes resource basis.