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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (17): 98-102.

所属专题： 生物技术

• 食品 营养 检测 安全 • 上一篇    下一篇

响应面法优化解脂耶罗维亚酵母ypy01淀粉酶发酵条件研究

柳志强 李晓宇 张横江 刘美珍   

  • 收稿日期:2010-11-09 修回日期:2011-01-05 出版日期:2011-07-15 发布日期:2011-07-15
  • 基金资助:

    海南省自然科学基金项目;海南大学科研启动基金项目

Optimization of Amylase Production from Yarrowia lipolytica ypy01 by Using Response Surface Methodology

  • Received:2010-11-09 Revised:2011-01-05 Online:2011-07-15 Published:2011-07-15

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摘要：

利用Plackett–Burman（PB）设计和响应面法对解脂耶罗维亚酵母ypy01淀粉酶生产条件进行了优化。PB设计筛选到3个显著因子，分别为可溶性淀粉浓度、蛋白胨浓度和初始pH。采用响应面法对3个显著因子进一步优化，获得了最佳培养基组成：0.84%可溶性淀粉，2.25%蛋白胨，1%酵母粉，3%NaCl，0.05% MgSO4，0.05% CaCl2，初始pH为6.8，在最优条件下，淀粉酶产量达到2012.50 U/mL。

关键词: POD, POD

Abstract:

The aim of this work was to optimize the operational parameters of marine yeast Yarrowia lipolytica ypy01 to increase amylase production using Plackett–Burman (PB) design and response surface methodology (RSM). The results of PB design showed that the concentrations of starch and peptone and initial pH were the most significant factors to influence amylase production. A Central Composite Design (CCD), which is the standard design of RSM, was then employed to further optimize these three factors. The experimental results indicated that the optimized composition of medium was 0.84% starch, 2.25% peptone, 1% yeast extract, 3% NaCl, 0.05% MgSO4, 0.05% CaCl2 and pH 6.8. Under the optimized conditions, the amylase production was up to 2012.50 U/mL in shake flask experiments.