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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (2): 104-108.

所属专题： 生物技术； 园艺

• 林学 园艺 园林 • 上一篇    下一篇

大蒜抗百草枯愈伤组织变异系的选择

张恩让 程智慧 杨伟林   

  • 收稿日期:2010-06-10 修回日期:2010-08-13 出版日期:2011-01-14 发布日期:2011-01-14

The Selection of Resistant Paraquat Callus Variation Line of Garlic

  • Received:2010-06-10 Revised:2010-08-13 Online:2011-01-14 Published:2011-01-14

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摘要：

选取‘改良蒜’、‘金堂早蒜’、‘苍山蒜’和‘欧引01’等4个不同生态型的大蒜品种，在含百草枯浓度分别为0.2%，0.4%，0.6%，0.8%，1.0%，1.5%的培养基上采用直接接种和逐级培养法，选择抗百草枯愈伤组织细胞系。结果表明：4个品种在低浓度（﹤0.4%）胁迫下都有一定的抗性，经逐级抗百草枯选择后的愈伤组织，其抗性能力有明显提高。在含有百草枯浓度0.6%培养基上，4个品种愈伤组织的成活率为7.8%~20.6%，且发育良好；在浓度0.8%和1.0%的百草枯培养基上也有部分愈伤组织成活。抗性稳定性检验表明，大蒜抗百草枯能力在一定时间内能够保持下去。

关键词: 马蹄金, 马蹄金, 立枯病, 毒力测定, 控制作用

Abstract:

Four different ecotypic garlic cultivars ‘modified garlic’, ‘Jintang early garlic’, ‘Cangshan’ and ‘Europe 01’ were chosen to select for the resistant paraquat callus, in the medium including paraquat concentrations 0.2%, 0.4%, 0.6%, 0.8%, 1.0% and 1.5%, respectively. The results showed that the four cultivars had some resistance under the low concentration stress (﹤0.4%), and the resistance of the callus were enhanced by step-to-step culturing method. The survival rates of the callus were from 7.8% to 20.6% in the medium including containing 0.6% paraquat. Some callus was also survived in the medium containing 0.8% and 1.0% Paraquat. Stability Test showed the ability of resistant paraquat could keep for a long time.