响应面法优化棕色棉纤维原花青素的提取工艺

doi:10.11924/j.issn.1000-6850.casb2020-0379

摘要/Abstract

摘要：

为了提高棕色棉纤维中原花青素的得率,优化原花青素的提取工艺。本文以棕色棉纤维为材料,研究甲醇浓度、料液比、超声时间和超声温度等单因素对原花青素提取率的影响,获得优化的提取条件。通过超声与甲醇-盐酸正丁醇相结合的方法提取原花青素,响应面法优化棕色棉纤维原花青素的提取工艺为：甲醇浓度83%,料液比1:60,超声温度42℃,超声时间18 min,提取率为(7.16±0.03)%,与一般方法相比,原花青素提取率提高4%左右。响应面法优化的提取工艺提取效率高,简单快速且可进行批量提取,为原花青素的生物活性研究和产品开发奠定基础。

关键词: 棕色棉, 棉纤维, 原花青素, 单因素, 响应面法, 提取工艺

Abstract:

To improve the yield of proanthocyanidin from brown cotton fiber, and optimize the extraction process, the brown cotton fibers were used as materials to study the effect of methanol concentration, solid-liquid ratio, ultrasonic time and temperature on the extraction efficiency of proanthocyanidin, and to obtain the optimized extraction conditions. Proanthocyanidin was extracted by ultrasonic combined with methanol-n-butanol hydrochloride, the extraction process was optimized by response surface methodology. The results showed that the optimum parameters were methanol concentration of 83%, solid-liquid ratio of 1:60, ultrasonic temperature of 42℃, ultrasonic time of 18 min, and the extraction rate was (7.16±0.03)%. The extraction yield of proanthocyanidin by the optimized method was improved by about 4% compared with that of the general method. The extraction process optimized by response surface methodology has high extraction efficiency, and the experimental operation is simple and fast, which can be used to extract proanthocyanidin in batches. The study lays a foundation for the research on biological activities and the development of products of proanthocyanidin.

Key words: brown cotton, cotton fiber, proanthocyanidin, single factor, response surface method, extraction process

中图分类号:

谢勇飞, 赵钢, 孙娜, 吕银萍, 吴勇, 沈美辰, 吕文犇, 高俊山. 响应面法优化棕色棉纤维原花青素的提取工艺[J]. 中国农学通报, 2021, 37(12): 136-143.

Xie Yongfei, Zhao Gang, Sun Na, Lv Yinping, Wu Yong, Shen Meichen, Lv Wenben, Gao Junshan. Proanthocyanidin Extraction Process from Brown Cotton Fiber: Optimized by Response Surface Method[J]. Chinese Agricultural Science Bulletin, 2021, 37(12): 136-143.

图/表 9

参考文献 34

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来源	平方和	自由度	均方	F值	P值	显著性
Model	75.30	14	5.38	3.00	0.0244^*	significant
A-甲醇浓度	6.27	1	6.27	3.49	0.0827
B-超声时间	3.27	1	3.27	1.82	0.1987
C-超声温度	33.84	1	33.84	18.86	0.0007^**
D-料液比	0.96	1	0.96	0.53	0.4767
AB	0.37	1	0.37	0.20	0.6581
AC	2.23	1	2.23	1.24	0.2837
AD	0.35	1	0.35	0.20	0.6637
BC	0.33	1	0.33	0.19	0.6731
BD	0.54	1	0.54	0.30	0.5919
CD	0.14	1	0.14	0.080	0.7815
A2	14.25	1	14.25	7.94	0.0137^*
B2	0.99	1	0.99	0.55	0.4702
C2	11.38	1	11.38	6.34	0.0246^*
D2	11.19	1	11.19	6.24	0.0256^*
Residual	25.13	14	1.79
Lack of Fit	22.19	10	2.22	3.02	0.1489	Not significant
Pure Error	2.94	4	0.73
Cor Total	100.43

来源	平方和	自由度	均方	F值	P值	显著性
Model	75.30	14	5.38	3.00	0.0244^*	significant
A-甲醇浓度	6.27	1	6.27	3.49	0.0827
B-超声时间	3.27	1	3.27	1.82	0.1987
C-超声温度	33.84	1	33.84	18.86	0.0007^**
D-料液比	0.96	1	0.96	0.53	0.4767
AB	0.37	1	0.37	0.20	0.6581
AC	2.23	1	2.23	1.24	0.2837
AD	0.35	1	0.35	0.20	0.6637
BC	0.33	1	0.33	0.19	0.6731
BD	0.54	1	0.54	0.30	0.5919
CD	0.14	1	0.14	0.080	0.7815
A2	14.25	1	14.25	7.94	0.0137^*
B2	0.99	1	0.99	0.55	0.4702
C2	11.38	1	11.38	6.34	0.0246^*
D2	11.19	1	11.19	6.24	0.0256^*
Residual	25.13	14	1.79
Lack of Fit	22.19	10	2.22	3.02	0.1489	Not significant
Pure Error	2.94	4	0.73
Cor Total	100.43