富硒菊芋多糖的提取及其体外抗氧化活性研究

doi:10.11924/j.issn.1000-6850.casb2020-0842

摘要/Abstract

摘要：

为了研究富硒菊芋多糖的提取工艺及其体外抗氧化活性,本研究以亚硒酸钠为硒源,采用盆栽施硒法对菊芋进行富硒培养;利用超声辅助法,在单因素试验的基础上,应用响应面法优化富硒菊芋多糖提取的影响因素（超声时间、超声功率和液料比）;在此基础上,探究富硒前后菊芋多糖对羟自由基(·OH)、1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-trinitrophenylhydrazine, DPPH)自由基及超氧阴离子自由基(O^2-·)清除率的影响。结果表明：菊芋能够吸收无机硒将其转化为有机硒;富硒菊芋多糖的最佳提取条件为超声时间(60 min)、超声功率(450 W)和液料比[25:1(mL/g)],硒多糖提取率最高(13.52%);富硒菊芋多糖(Se-Inulin)和普通菊糖(Inulin)对羟基自由基、DPPH自由基及超氧自由基的清除活性均呈现出良好的量效关系,最大清除率可分别达到80.34%、89.19%与88.54%,Se-Inulin的抗氧化活性优于Inulin。本研究为富硒菊芋产品的开发提供了一定的理论依据。

关键词: 硒, 菊糖, 超声提取, 响应面法, 抗氧化活性

Abstract:

To study the extraction processes of rich selenium inulin polysaccharide extraction and its vitro antioxidant activities, this study used sodium selenite as the selenium source, add sodium selenite to potting soil for selenium-enriched culture of Jerusalem artichoke. Based on the single factor experiment, the response surface methodology was applied to optimize the factors affecting the extraction of selenium-enriched Jerusalem artichoke polysaccharide (ultrasonic time, ultrasonic power, liquid-to-solid ratio). Then, the scavenging ability of Se-enriched and non-Se-enriched Jerusalem artichoke polysaccharide were explored to the hydroxyl radical,1,1-diphenyl-2-trinitrophenylhydrazine(DPPH) and superoxide anion. The results showed that the Jerusalem artichoke could positively absorb selenium and convert it to organic selenium. The optimal extraction condition was: ultrasonic time of 60 min, ultrasonic power of 450 W and liquid-to-solid ratio of 25:1 (mL/g), which could attain the highest extraction rate of Se-inulin (13.52%). Selenium-enriched Jerusalem artichoke polysaccharide (Se-inulin) and ordinary inulin (inulin) showed a good dose-effect relationship on hydroxyl radical, DPPH radical and superoxide radical scavenging activity, and the maximum clearance rate could reach 80.34%, 89.19% and 88.54%, respectively. The antioxidant activity of Se-inulin was better than that of inulin. In conclusion, this study could provide a theoretical basis for the future development of Se-enriched Jerusalem artichoke products.

Key words: Se, inulin, ultrasonic extraction, response surface methodology, antioxidant activity

中图分类号:

Q946.3

许丹丹, 徐雅琴, 隽行, 周守标, 汪昌保, 杭华. 富硒菊芋多糖的提取及其体外抗氧化活性研究[J]. 中国农学通报, 2021, 37(30): 121-127.

Xu Dandan, Xu Yaqin, Juan Xing, Zhou Shoubiao, Wang Changbao, Hang Hua. Optimization of Extraction Process of Se-enriched Jerusalem artichoke Polysaccharides and Its Antioxidant Activity[J]. Chinese Agricultural Science Bulletin, 2021, 37(30): 121-127.

图/表 10

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因素	水平
因素	-1	0	1
A超声时间/min	55	60	65
B超声功率/W	400	450	500
C液料比(mL/g)	15:01	25:01:00	35:01:00

因素	水平
因素	-1	0	1
A超声时间/min	55	60	65
B超声功率/W	400	450	500
C液料比(mL/g)	15:01	25:01:00	35:01:00

采样		不同处理硒积累量/(mg/kg)
时间/d	植株部位	空白	15	25	50	75	100
80	块茎	0.003±0.02a	0.699±0.11a	0.989±1.67b	1.451±4.57c	3.872±6.72d	7.132±8.19e
150	块茎	0.121±0.02a	0.757±0.13b	0.124±1.87c	1.853±3.94d	5.352±7.34e	8.563±8.83f

采样		不同处理硒积累量/(mg/kg)
时间/d	植株部位	空白	15	25	50	75	100
80	块茎	0.003±0.02a	0.699±0.11a	0.989±1.67b	1.451±4.57c	3.872±6.72d	7.132±8.19e
150	块茎	0.121±0.02a	0.757±0.13b	0.124±1.87c	1.853±3.94d	5.352±7.34e	8.563±8.83f

变异来源	平方和	自由度	均方	F值	P值	显著性
模型	46.50	9	5.17	678.72	<0.0001	***
A超声时间	0.032	1	0.032	4.18	0.0682	不显著
B超声功率	0.00364	1	0.00364	0.40	0.5401	不显著
C液料比	11.24	1	11.24	1477.12	<0.0001	***
AB	0.40	1	0.40	52.61	<0.0001	***
AC	0.51	1	0.51	66.34	<0.0001	***
BC	0.16	1	0.16	20.97	0.0010	*
A²	19.72	1	19.72	2590.34	<0.0001	***
B²	3.47	1	3.47	456.25	<0.0001	***
C²	16.47	1	16.47	2163.29	<0.0001	***
残差	0.076	10	0.00761
失拟项	0.010	5	0.002	0.16	0.9676
误差项	0.066	5	0.013
总和	46.58	19