欢迎访问《中国农学通报》,

中国农学通报 ›› 2022, Vol. 38 ›› Issue (30): 6-13.doi: 10.11924/j.issn.1000-6850.casb2021-1213

所属专题: 生物技术 水稻

• 农学·农业基础科学 • 上一篇    下一篇

基于SNP标记的穞稻与栽培稻的遗传多样性分析及萌发耐淹性评价

孙志广1(), 潘根2, 陈庭木1, 李景芳1, 赵利君3, 迟铭1, 徐波1, 邢运高1, 刘金波1, 刘晓敏1, 葛高宁4, 徐锦涛1, 王宝祥1(), 徐大勇1()   

  1. 1连云港市农业科学院/江苏省现代作物生产协同创新中心,江苏连云港 222006
    2中国农业科学院麻类研究所,长沙 410000
    3南京农业大学生命科学学院,南京 210095
    4苏州农业职业技术学院环境工程学院,江苏苏州 215026
  • 收稿日期:2021-12-28 修回日期:2022-05-02 出版日期:2022-10-25 发布日期:2022-10-27
  • 通讯作者: 王宝祥,徐大勇
  • 作者简介:孙志广,男,1989年出生,河南周口人,副研究员,博士,研究方向:水稻遗传育种。通信地址:222002 江苏省连云港市海州区郁州南路与迎宾大道交叉口 连云港市农业科学院,Tel:0518-83081733,E-mail: zhiguangsun@126.com
  • 基金资助:
    国家现代农业产业技术体系建设专项“适合轻简化生产的水稻品种培育”(CARS-01-01A);江苏省农业重大品种创制“早熟优质抗稻瘟病水稻新品种选育”(PZCZ201704);江苏省自然科学基金“水稻黑条矮缩病抗性基因qRBSDD3K的克隆与功能分析”(BK20201214);湖南省自然科学基金青年基金“细胞分裂素氧化酶OsCKX4参与水稻干旱胁迫响应的功能研究”(2019JJ50714);连云港市财政专项“水稻抗逆基因的挖掘和育种利用”(QNJJ2001);“连云港穞稻优异基因的挖掘”(QNJJ2203)

Ludao and Cultivated Rice: Genetic Diversity Analysis and Germinability Evaluation Under Submerged Condition by SNP Markers

SUN Zhiguang1(), PAN Gen2, CHEN Tingmu1, LI Jingfang1, ZHAO Lijun3, CHI Ming1, XU Bo1, XING Yungao1, LIU Jinbo1, LIU Xiaomin1, GE Gaoning4, XU Jintao1, WANG Baoxiang1(), XU Dayong1()   

  1. 1Lianyungang Academy of Agricultural Sciences/Jiangsu Collaborative Innovation Center for Modern Crop Production, Lianyungang, Jiangsu 222006
    2Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410000
    3College of Life Sciences, Nanjing Agricultural University, Nanjing 210095
    4College of Environmental Engineering, Suzhou Polytechnic Institute of Agriculture, Suzhou, Jiangsu 215026
  • Received:2021-12-28 Revised:2022-05-02 Online:2022-10-25 Published:2022-10-27
  • Contact: WANG Baoxiang,XU Dayong

摘要:

旨在分析穞稻与栽培稻间的遗传多样性,为穞稻的保护和开发利用提供分子依据。以包括穞稻在内的48份水稻种质资源为试验材料,利用1K mGPS SNP芯片进行基因分型,使用PowerMaker 3.25软件进行UPGMA聚类分析。结果表明,4785对多态性SNP标记共检测出9570个等位基因,平均每对标记检测出2个等位变异;多态性信息含量(PIC)变化范围为0.0400~0.5957,平均值为0.3123,基因多样性指数(GD)变化范围为0.0408~0.6667,平均值为0.3839。UPGMA聚类分析将48份参试种质划分为2个亚群,即籼稻亚群和粳稻亚群,其中穞稻分属于粳稻亚群。厌氧萌发耐受性评价表明,穞稻具有很强的耐低氧萌发能力,可为适宜直播水稻的培育提供重要的种质基础,同时本研究为穞稻优异基因的挖掘和利用提供理论基础。

关键词: 穞稻, 栽培稻, SNP, 遗传多样性, 萌发耐淹性

Abstract:

The research aims at analyzing genetic diversity between Ludao and cultivated rice, and providing molecular reference for the protection, exploitation and utilization of Ludao. Forty-eight germplasm resources, including Ludao, were selected as experimental materials. A 1K mGPS SNP chip was used for genotyping. Cluster analysis based on the unweighted pair group method of mathematical averages (UPGMA) was performed using PowerMaker 3.25 software. The results showed that a total of 9570 alleles were detected using 4785 pairs of polymorphic SNP markers. The mean number of alleles detected for each locus was 2.0. The polymorphism information content (PIC) ranged from 0.0400 to 0.5957, with a mean value of 0.3123. The gene diversity index (GD) ranged from 0.0408 to 0.6667, with a mean value of 0.3839. UPMGA cluster analysis separated the 48 accessions into two subgroups (indica and japonica), and Ludao belonged to the japonica subgroup. The evaluation of anaerobic germination tolerance showed that Ludao displayed high tolerance to hypoxia during germination, which could provide an important germplasm base for breeding new cultivars suitable for direct seeding. The results presented here also provide a theoretical basis for exploiting and utilizing the elite genes of Ludao.

Key words: Ludao, cultivated rice, SNP, genetic diversity, germinability under submerged condition

中图分类号: