一个新的水稻黄绿叶突变体ygl-9108的鉴定与基因定位

doi:10.11924/j.issn.1000-6850.casb2023-0549

中国农学通报 ›› 2024, Vol. 40 ›› Issue (26): 22-29.doi: 10.11924/j.issn.1000-6850.casb2023-0549

一个新的水稻黄绿叶突变体ygl-9108的鉴定与基因定位

刘晓敏(), 孙志广(), 迟铭, 邢运高, 徐波, 李景芳, 刘艳, 卢百关, 王宝祥, 徐大勇()

连云港市农业科学院/江苏省现代作物生产协同创新中心，江苏连云港 222000

收稿日期:2023-08-15 修回日期:2023-11-16 出版日期:2024-09-11 发布日期:2024-09-11
通讯作者:
徐大勇，男，1964年出生，江苏连云港人，研究员，博士，研究方向：农作物新品种选育和推广。通信地址：222002 江苏省连云港市海州区郁州南路与迎宾大道交叉口连云港市农业科学院，Tel：0518-85807468，E-mail：xudayong3030@sina.com；
孙志广，男，1989年出生，河南周口人，副研究员，博士，研究方向：水稻遗传育种。通信地址：222002 江苏省连云港市海州区郁州南路与迎宾大道交叉口连云港市农业科学院，Tel：0518-83081733，E-mail：zhiguangsun@126.com。
作者简介:
刘晓敏，女，1993年出生，江苏连云港人，研究实习员，学士，研究方向：水稻遗传育种。通信地址：222000 江苏省连云港市海州区郁州南路与迎宾大道交叉口连云港市农业科学院，Tel：0518-83081733，E-mail：824659317@qq.com。
基金资助:
国家现代农业产业技术体系建设专项“适合轻简化生产的水稻品种培育”(CARS-01-01A); 江苏省种业振兴揭榜挂帅项目“抗稻瘟病外观透明优质食味早熟晚粳稻新品种培育”(JBGS[2021]039); 连云港市财政专项“水稻粉质突变体m149表型分析及基因定位”(QNJJ2311); “连云港穞稻优异基因的挖掘”(QNJJ2203)

Identification and Gene Mapping of A Novel Yellow-green Leaf Mutant ygl-9108 in Rice

LIU Xiaomin(), SUN Zhiguang(), CHI Ming, XING Yungao, XU Bo, LI Jingfang, LIU Yan, LU Baiguan, WANG Baoxiang, XU Dayong()

Lianyungang Academy of Agricultural Sciences/Jiangsu Collaborative Innovation Center for Modern Corp Production, Lianyungang, Jiangsu 222000

Received:2023-08-15 Revised:2023-11-16 Published:2024-09-11 Online:2024-09-11

摘要/Abstract

摘要：

本研究旨在对水稻黄绿叶突变体ygl-9108进行表型分析和基因定位，为后续图位克隆该叶色相关基因奠定基础。以黄绿叶突变体ygl-9108为试材，利用突变体ygl-9108和‘五山丝苗’杂交的F₂群体进行基因定位。结果表明，与野生型相比，突变体的株高、有效穗数、穗长、每穗粒数、结实率、粒宽、千粒重和单株谷重均显著下降，下降比例分别为21.5%、21.2%、14.6%、19.2%、11.0%、10.2%、12.9%和52.2%。透射电镜观察显示，突变体叶肉细胞的叶绿体数量大量减少，类囊体结构异常。遗传分析表明，突变体的表型由一个隐性核基因控制。利用图位克隆方法，ygl-9108被定位于水稻第11号染色体两InDel标记11Y39和11Y45之间，物理距离约为147 kb，该区间内未见有叶色相关基因的报道，表明ygl-9108是一个新的黄绿叶调控基因。本研究结果为ygl-9108的克隆和功能分析奠定了坚实的基础。

关键词: 水稻, 黄绿叶突变体, ygl-9108, 表型鉴定, 农艺性状, 基因定位, 透射电镜, 叶绿体, 隐性核基因, 图位克隆

Abstract:

The research aims to lay the foundation for map-based cloning of the leaf color related gene by analyzing the phenotypic characteristics of a rice yellow-green leaf mutant ygl-9108 and mapping the mutant locus. A yellow-green leaf mutant, named as ygl-9108, was selected as experimental material, and the gene was mapped by using the F₂ population derived from the cross between the ygl-9108 mutant and ‘Wushansimiao’. The results showed that the plant height, effective panicle number, panicle length, grain number per panicle, seed setting rate, grain width, 1000-grain weight, and grain weight per plant of the ygl-9108 mutant were all significantly reduced by 21.5%, 21.2%, 14.6%, 19.2%, 11.0%, 10.2%, 12.9%, and 52.2%, respectively, compared with those of the wild type. The observation by transmission electronic microscope (TEM) demonstrated that the number of chloroplasts in mesophyll cells of ygl-9108 plants was greatly reduced, and the lamellar structure of chloroplasts was abnormal. Genetic analysis revealed that the phenotype of mutant was controlled by a single recessive nuclear gene. Using a map-based cloning strategy, ygl-9108 was mapped to a 147 kb region on chromosome 11 between two InDel markers, 11Y39 and 11Y45. No gene related to leaf color has been reported previously in this interval, indicating that ygl-9108 is a new leaf color-related gene. This study lays a solid foundation for further research on gene cloning and function analysis of ygl-9108.

Key words: Oryza sativa L., yellow-green leaf mutants, ygl-9108, phenotypic identification, agronomic trait, gene mapping, transmission electron microscope, chloroplast, recessive nuclear gene, map-based cloning

刘晓敏, 孙志广, 迟铭, 邢运高, 徐波, 李景芳, 刘艳, 卢百关, 王宝祥, 徐大勇. 一个新的水稻黄绿叶突变体ygl-9108的鉴定与基因定位[J]. 中国农学通报, 2024, 40(26): 22-29.

LIU Xiaomin, SUN Zhiguang, CHI Ming, XING Yungao, XU Bo, LI Jingfang, LIU Yan, LU Baiguan, WANG Baoxiang, XU Dayong. Identification and Gene Mapping of A Novel Yellow-green Leaf Mutant ygl-9108 in Rice[J]. Chinese Agricultural Science Bulletin, 2024, 40(26): 22-29.

图/表 5

图1. 野生型(WT)和突变体ygl-9108的表型鉴定 A：分蘖期野生型(WT)和突变体(ygl-9108)的植株表型；B-E：分蘖期野生型(WT)和突变体(ygl-9108)的剑叶、倒二叶、倒三叶和基部叶片；F-G：野生型(WT)和突变体(ygl-9108)单株产量的比较，稻谷(F)和糙米(G)；H-I：野生型(WT)和突变体(ygl-9108)种子宽度和长度的比较；J-K：野生型(WT)和突变体(ygl-9108)植株主茎穗以及不同节间长度的比较。标尺：5 cm (A), 2 cm (B-E), 4 cm (F), 3 cm (G), 1 cm (H-I), 10 cm (J)

图2. 野生型(WT)和突变体ygl-9108的农艺性状比较 A：株高；B：有效穗数；C：每穗粒数；D：结实率；E：穗长；F：千粒重；G：粒宽；H：单株谷重；I：粒长；J：粒厚；K：抽穗期，**表示在0.01水平上差异显著，ns表示无显著性差异

图3. 野生型(WT)和突变体ygl-9108抽穗期叶片的光合色素含量抽穗期野生型(WT)和突变体ygl-9108的剑叶(A)、倒二叶(B)、倒三叶(C)和基部叶片(D)的光合色素含量，**表示在0.01水平上差异显著，ns表示无显著性差异

图4. 野生型(WT)和突变体ygl-9108的叶肉细胞超微结构 A：成熟期野生型(WT)剑叶叶片；B-D：成熟期野生型(WT)剑叶细胞超微结构；E：成熟期突变体ygl-9108剑叶叶片；F-H：成熟期突变体ygl-9108剑叶细胞超微结构。标尺：1 cm (A, E)，40 μm (B, F)，10 μm (C, G)，2 μm (D, H)。

图5. ygl-9108基因在水稻第11染色体上的分子定位

参考文献 34

[1]	滕炎桐, 叶莲, 何福收, 等. 一个水稻黄叶突变体的叶绿素合成关键基因的表达分析[J]. 中国农学通报, 2017, 33(22):30-35. doi: 10.11924/j.issn.1000-6850.casb17040104
[2]	ECKHARDT U, GRIMM B, HORTENSTEINER S. Recent advances in chlorophyll biosynthesis and breakdown in higher plants[J]. Plant molecular biology, 2004, 56(1):1-14. doi: 10.1007/s11103-004-2331-3 pmid: 15604725
[3]	李哲理, 张林金, 谭颖, 等. 水稻白叶白穗突变体wlwp7的鉴定与基因定位[J]. 热带作物学报, 2021, 42(9):2512-2517. doi: 10.3969/j.issn.1000-2561.2021.09.010
[4]	孙志广, 代慧敏, 陈庭木, 等. 水稻类病斑突变体lmm7的鉴定与基因定位[J]. 中国水稻科学, 2022, 36(4):357-366. doi: 10.16819/j.1001-7216.2022.210711
[5]	ASAKURA Y, HIROHASHI T, KIKUCHI S, et al. Maize mutants lacking chloroplast FtsY exhibit pleiotropic defects in the biogenesis of thylakoid membranes[J]. Plant cell, 2004, 16:201-214. pmid: 14688289
[6]	TANG W J, WANG W Q, CHEN D Q, et al. Transposase derived proteins FHY3/FAR1 interact with PHYTOCHROME - INTERACTING FACTOR1 to regulate chlorophyll biosynthesis by modulating HEMB1 during deyellow in Arabidopsis[J]. Plant cell, 2012, 24(5):1984-2000.
[7]	欧点点, 赵光伟, 贺玉花, 等. 甜瓜果皮颜色遗传分析及基因定位[J]. 中国农学通报, 2019, 35(13):64-69. doi: 10.11924/j.issn.1000-6850.casb18110127
[8]	DENG X J, ZHANG H Q, WANG Y, et al. Mapped clone and functional analysis of leaf-color gene Ygl7 in a rice hybrid (Oryza sativa L.)[J]. PLos one, 2014, 9(6):e99564.
[9]	刘艳霞, 林冬枝, 董彦君, 等. 水稻温敏感叶色突变体研究进展[J]. 中国水稻科学, 2015, 29(4):439-446. doi: 10．3969/j．issn．1001G7216．2015．04．014
[10]	林秋云, 谢振宇, 龙开意, 等. 水稻黄叶不育系突变体H08S的表型特征与遗传分析[J]. 热带作物学报, 2020, 41(7):1321-1325. doi: 10.3969/j.issn.1000-2561.2020.07.006
[11]	KUSUMI K, KOMORI H, SATOH H, et al. Characterization of a zebra mutant of rice with increased susceptibility to light stress[J]. Plant and cell physiology, 2000, 41(2):158-164. pmid: 10795309
[12]	WANG P Y, LI C M, WANG Y, et al. Identification of a geranylgeranyl reductase gene for chlorophyll synthesis in rice[J]. Springerplus, 2014, 3:201. doi: 10.1186/2193-1801-3-201 pmid: 24809003
[13]	YANG Y L, XU J, RAO Y C, et al. Cloning and functional analysis of pale-green leaf (PGL10) in rice (Oryza sativa L.)[J]. Plant growth regulation, 2016, 78(1):69-77.
[14]	WU Z, ZHANG X, HE B, et al. A chlorophyll-deficient rice mutant with impaired chlorophyllide esterification in chlorophyll biosynthesis[J]. Plant physiology, 2007, 145(1):29-40. doi: 10.1104/pp.107.100321 pmid: 17535821
[15]	TAN J J, TAN Z H, WU F Q, et al. A novel chloroplast-localized pentatricopeptide repeat protein involved in splicing affects chloroplast development and abiotic stress response in rice[J]. Molecular plant, 2014, 7(8):1329-1349. doi: S1674-2052(14)60936-9 pmid: 24821718
[16]	平文超, 徐婧, 曹平平, 等. 外源钾对盐胁迫下植物生长和生理特征影响的研究进展[J]. 中国农学通报, 2023, 39(9):100-105. doi: 10.11924/j.issn.1000-6850.casb2022-0098
[17]	CHEN N, WANG P, LI C, et al. A single nucleotide mutation of the IspE gene participating in the MEP pathway for isoprenoid biosynthesis causes a green-revertible yellow leaf phenotype in rice[J]. Plant and cell physiology, 2018, 59(9):1905-1917.
[18]	HUANG R, WANG Y, WANG P, et al. A single nucleotide mutation of IspF gene involved in the MEP pathway for isoprenoid biosynthesis causes yellow-green leaf phenotype in rice[J]. Plant molecular biology, 2018, 96:5-16.
[19]	SU N, HU M L, WU D X, et al. Disruption of a rice pentatricopeptide repeat protein causes a seedling-specifc albino phenotype and its utilization to enhance seed purity in hybrid rice production[J]. Plant physiology, 2012, 159(1):227-238.
[20]	LICHTENTHALER H K. Chlorophylls and carotenoids: pigments of photosynthetic biomembranes[J]. Methods in enzymology, 1987, 148:350-382.
[21]	FANG L K, LI Y F, GONG X P, et al. Genetic analysis and gene mapping of a dominant presenescing leaf gene PSL3 in rice (Oryza sativa L.)[J]. Chinese science bulletin, 2010, 55:2517-2521.
[22]	SAKURABA Y, PARK S Y, KIM Y S, et al. Arabidopsis STAY-GREEN2 is a negative regulator of chlorophyll degradation during leaf senescence[J]. Molecular Plant, 2014, 7(8):1288-1302.
[23]	ZHU X W, PAN Y, LIU Z, et al. Mutation of YL results in a yellow leaf with chloroplast RNA editing defect in soybean[J]. International journal of molecular sciences, 2020, 21(12):4275.
[24]	MIAO H, ZHANG S, WANG M, et al. Fine mapping of virescent leaf gene v-1 in cucumber (Cucumis sativus L.)[J]. International journal of molecular sciences, 2016, 17(10):1602.
[25]	MAO D H, YU H H, LIU T M, et al. Two complementary recessive genes in duplicated segments control etiolation in rice[J]. Theoretical and applied genetics, 2011, 122(2):373-383. doi: 10.1007/s00122-010-1453-z pmid: 20872210
[26]	ZHANG F, LUO X, HU B, et al. YGL138(t), encoding a putative signal recognition particle 54 kDa protein, is involved in chloroplast development of rice[J]. Rice, 2013, 6(1):1-10.
[27]	邓晓梅, 叶胜海, 修芬连, 等. 一个水稻黄绿叶突变性状的遗传分析及基因定位[J]. 核农学报, 2012, 26(2):203-209. doi: 10.11869/hnxb.2012.02.0203
[28]	CHI X F, ZHOU X S, SHU Q Y. Fine mapping of a Xantha mutation in rice (Oryza sativa L.)[J]. Euphytica, 2010, 172(2):215-220.
[29]	LI Q Z, ZHU F Y, GAO X L, et al. Young leaf chlorosis 2 encodes the stroma-localized heme oxygenase 2 which is required for normal tetrapyrrole biosynthesis in rice[J]. Planta, 2014, 240(4):701-712. doi: 10.1007/s00425-014-2116-0 pmid: 25037719
[30]	江少华, 周华, 林冬枝, 等. 水稻幼苗叶色温敏感突变体的鉴定及其基因定位[J]. 中国水稻科学, 2013, 27(4):359-364.
[31]	王文娟, 吴兰兰, 雷晓庆, 等. 一个新水稻低温敏感叶色突变体tcm11的鉴定及基因定位[J]. 上海师范大学学报(自然科学版), 2017, 46(2):319-325.
[32]	吴书俊, 王军, 杨杰, 等. 水稻黄绿叶突变体ygl11(t)的生理特性和基因克隆[J]. 中国水稻科学, 2015, 29(2):111-118. doi: 10.3969/j.issn.1001-7216.2015.02.001
[33]	LI R Q, HUANG J Z, ZHAO H J, et al. A down-regulated epi-allele of the genomes uncoupled 4 gene generates a xantha marker trait in rice[J]. Theoretical and applied genetics, 2014, 127(11):2491-2501.
[34]	李自壮, 徐乾坤, 余海平, 等. 水稻淡黄叶矮化突变体yld的遗传分析及基因定位[J]. 作物学报, 2017, 43(4):522-529.

一个新的水稻黄绿叶突变体ygl-9108的鉴定与基因定位

Identification and Gene Mapping of A Novel Yellow-green Leaf Mutant ygl-9108 in Rice

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