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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (16): 136-141.

所属专题： 园艺

• 林学 园艺 园林 • 上一篇    下一篇

基于RAPD标记与形态标记的辣椒自交系间亲缘关系的比较研究

罗英 吴立东 曾绍贵 朱邦彤 许旭明 温庆放   

  • 收稿日期:2011-01-10 修回日期:2011-02-28 出版日期:2011-07-05 发布日期:2011-07-05
  • 基金资助:

    福建省科技厅重点项目“加工专用型优高辣椒良种选育与示范”[2007N0092]资助。

Study on pepper inbred lines′ relationship based on RAPD technique and morphological clustering

  • Received:2011-01-10 Revised:2011-02-28 Online:2011-07-05 Published:2011-07-05

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摘要：

为了更加准确地测定辣椒亲本间的亲缘关系，提高辣椒育种效率。利用RAPD标记技术对60份辣椒自交系进行了基因组多态性分析，并将形态学聚类和分子鉴定加以对比。结果表明：（1）对60个辣椒自交系的12个数量性状进行了聚类分析，遗传距离在4.8时，将其分为5类。（2）利用RAPD标记对60份辣椒自交系进行了基因组多态性分析，遗传距离D值在0.35处，将辣椒的3个变种基本分开。（3）RAPD标记计算的遗传相异系数矩阵和形态学计算的遗传距离矩阵做相关性分析，其相关系数为0.26，达到极显著。因此，在研究辣椒自交系亲缘关系时，可将两者相结合进行遗传相似性的分析，提高其准确性。

关键词: 遗传多样性, 遗传多样性

Abstract:

To more accurately determine the relationships between pepper parents and improve the pepper breeding efficiency, 60 pepper inbred lines were used to analyze genomic polymorphism by RAPD and morphological clustering. The results of morphological clustering with molecular identification were compared too. The results showed: (1) 60 pepper inbred lines were classified into five groups at the genetic distance level of 4.8 that were calculated by 12 agronomic quantitative traits. (2) 60 pepper inbred lines were classified into three groups at the genetic distance level of D 0.35 that were calculated by RAPD. (3) The results of the morphological clustering and molecular identification by RAPD were similarly, and the correlation index was 0.26, significant. All in all, the two methods could be combined to study the relationships between pepper parents. It could improve the accurately of genetic similarity.