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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (19): 137-143.

所属专题： 园艺

• 林学 园艺 园林 • 上一篇    下一篇

大白菜的类胡萝卜素种类鉴定

陈鹏飞 刘栓桃 张志刚 李巧云 赵智中 薛林宝   

  • 收稿日期:2011-01-14 修回日期:2011-03-19 出版日期:2011-08-05 发布日期:2011-08-05
  • 基金资助:

    山东省农业科学院重大成果培育计划

Category Identification and Analysis of Carotenoids in Chinese Cabbage

  • Received:2011-01-14 Revised:2011-03-19 Online:2011-08-05 Published:2011-08-05

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摘要：

为建立大白菜类胡萝卜素鉴定方法，利用反相高效液相色谱（RP-HPLC），根据标样和二极管阵列检测器的检测结果，对不同类型大白菜材料的类胡萝卜素种类进行鉴定。结果表明：利用C30柱从大白菜中共分离出48类胡萝卜素，根据保留时间和吸收谱特征鉴定出其中的30种成分，链孢红素、玉米黄质、六氢番茄红素、ζ-胡萝卜素、γ-胡萝卜素和紫黄质均为首次在大白菜中报道。橙色大白菜中的类胡萝卜素种类最多，与其他大白菜存在很大差异。说明该鉴定方法适用于大白菜的类胡萝卜素种类研究。

关键词: 序列分析, 序列分析

Abstract:

To establish a method for identification of carotenoids in chinese cabbage, reversed phase high-performance liquid chromatography (RP-HPLC), coupled with carotenoid standards and photodiode array detection was used to analyze caotenoid composition in different genotypes of chinese cabbage. 48 kinds of carotenoids were separated on a C30 column, and 30 of them were identified on the basis of retention time and spectral characteristics with standards. Among which neurosporene, zeaxanthin, phytofluene, ζ-carotene, γ-carotene and violaxanthinand were first identified from chinese cabbage. The kinds of carotenoid varied among genotypes and those in orange-heading chinese cabbage were the most. The results also indicated that this method was suitable for identification of carotenoids in chinese cabbage.