关键词: 银杏, 银杏, 正交试验, 愈伤组织, 银杏叶汁, 银杏胚乳汁

Abstract:

The genetic diversity of 48 rapeseed (Brassica napus L.), which were from domestic and foreign, was analyzed by SSR markers. The results showed that 45 pairs of primers performed 326 allele loci, and the percentage of polymorphism locus was 86.2% with 281 loci was detected. The average number of allele and polymorphism loci per primer was 7.2 and 6.2, respectively. The range of PIC per primer was 0.374~0.856, with the average of 0.699. Difference among the accessions was large with the genetic similarity range 0.48~0.79. To set 0.51 as the threshold, all 48 accessions were divided into 3 groups, as winter group, weak-winter group and spring group. The groups were independent, but still had degree of mutual inter-penetration. That was to say the accessions might have some genetic relationship. The result of PCA and cluster analysis was consistent. It concluded that SSR could reveal the genetic diversity of germplasm, provide help for preservation of germplasm, and analyze genetic diversity of breeding material. It had important reference for breeding.