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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (23): 1-5.

所属专题： 生物技术

• 畜牧 动物医学 蚕 蜂 •    下一篇

分子生物学技术在瘤胃厌氧真菌分类多样性与定量分析研究中的应用

杨红建 沈博通 曹阳春   

  • 收稿日期:2011-03-31 修回日期:2011-04-25 出版日期:2011-09-15 发布日期:2011-09-15
  • 基金资助:

    国家自然科学基金委面上项目

Application Advances of Modern Molecular Techniques in Microbial Diversity and Quantitative Analysis of Anaerobic Fungi in the Rumen

  • Received:2011-03-31 Revised:2011-04-25 Online:2011-09-15 Published:2011-09-15

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摘要：

栖居在瘤胃中的厌氧真菌对饲料纤维的降解具有重要的促进作用。本文就瘤胃厌氧真菌的生物学分类以及生活史的国内外研究进展进行了综述分析。基于核糖体DNA的序列分析、限制性片段长度多态性（RFLP）、核糖体间隔基因自动分析（ARISA）、等分子生物学技术的快速发展与应用为厌氧真菌研究开辟了新的途径。这些快速、易于操作的新技术，因其所具有高度灵敏性和特异性，而被广泛用于对瘤胃厌氧真菌的分类鉴定和系统进化分析中，并通过实时定量PCR技术目前已经实现了瘤胃微生态环境下的真菌生物量检测。

关键词: 保护酶, 保护酶

Abstract:

Anaerobic fungi play a significant role in promoting the degradation of fibrous feed in the rumen. Authors reviewed recent research advances in biological taxonomy, classification and life circle of rumen fungi. The rapid development and application of molecular biology methods, including rDNA sequence analysis, restriction fragment length polymorphisms (RFLP) and automated ribosomal intergenic spacer analysis (ARISA), have brought a new pathway for updating the study of rumen fungi. Due to high sensitivity and specificity, these easy-to-handle techniques have been widely applied in the taxonomical classification and molecular phylogenetic analysis for rumen fungi, and quantitative analysis of the fungi biomass by a real-time polymerase chain reaction (PCR) technique has been successfully used to monitor anaerobic fungi in rumen micro-ecology circumstance.