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中国农学通报

中国农学通报 ›› 2015, Vol. 31 ›› Issue (20): 71-75.doi: 10.11924/j.issn.1000-6850.casb15040015

所属专题： 生物技术

• 生物技术科学 • 上一篇    下一篇

Cry1Ab蛋白在果蝇体内的富集作用及对其体内保护酶活性的影响

李秦晋1,2,魏宝阳1,王 智1   

  1. (1湖南农业大学生物科学技术学院,长沙 410128；2湖南体育职业学院,长沙 410019）
  • 收稿日期:2015-04-02 修回日期:2015-07-06 接受日期:2015-06-25 出版日期:2015-07-28 发布日期:2015-07-28
  • 通讯作者: 李秦晋
  • 基金资助:
    2011年国家自然科学基金项目面上项目“转Bt基因水稻对稻田蜘蛛发育与繁殖的影响研究”(31071943)；2014年湖南省科技厅重点项目“稻田蜘蛛优势种蜕皮激素调控作用机理及应用研究”(2014FJ2003)。

Bio-concentration of Cry1Ab Protein in Drosophila melanogaster and Its Effect on Endogenous Enzymes of Protective System

Li Qinjin1,2, Wei Baoyang1, Wang Zhi1   

  1. (1College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128;2Hunan Sports Vocational College, Changsha 410019)
  • Received:2015-04-02 Revised:2015-07-06 Accepted:2015-06-25 Online:2015-07-28 Published:2015-07-28

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摘要： 为了研究Cry1Ab蛋白对非靶标生物的生态安全性，通过采用酶联免疫技术(ELISA)测定了果蝇体内的Cry1Ab蛋白含量，运用紫外分光光度法检测了超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、乙酰胆碱酯酶(AChE)的活性。结果表明：在前10天内Cry1Ab蛋白在果蝇体内不断累积，果蝇体内的AChE、SOD和GSH-Px酶活性与对照相比均受到抑制，SOD和GSH-Px酶活性的抑制程度随其体内毒蛋白含量的增高而降低，AChE酶活性的抑制程度则随之而升高；但10天后果蝇体内Cry1Ab蛋白含量逐渐降低，其体内3种酶的活性均随体内毒蛋白含量的降低而逐渐升高，但仍低于对照。该研究表明Cry1Ab蛋白在果蝇体内具有富集效应，并且其体内Cry1Ab蛋白含量对其体内SOD、GSH-Px、AChE具有抑制作用。

关键词: 辣椒, 辣椒, 分子标记, 研究进展

Abstract: To study the effects of Cry1Ab protein on ecology security of non-target organisms, enzyme-linked immunosorbent assay (ELISA) was applied to test Cry1Ab protein of Drosophila melanogaster and ultraviolet spectroscopy was applied to test the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and acetylcholinesterase (AChE) in Drosophila melanogaster. The results showed that Cry1Ab protein continuously accumulated in the first 10 days. Compared with the normal level, specific activity of AChE, SOD and GSH-Px were inhabited. The inhibition on the activity of AChE was strengthened, and the activities of GSH-Px and SOD were reduced with the increasing content of Cry1Ab protein. After 10 days, content of poisonous protein was reduced, inhibitions on AChE, SOD and GSH-Px were reduced, but activities of the three enzymes were still lower than those of the control groups. These results suggest that there is bio- concentration of Cry1Ab protein and specific activity of AChE, SOD, GSH-Px can be inhabited.