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中国农学通报 ›› 2016, Vol. 32 ›› Issue (8): 54-57.doi: 10.11924/j.issn.1000-6850.casb15110053

所属专题: 生物技术

• 植物保护 农药 • 上一篇    下一篇

宁乡地区斜纹夜蛾3种保护酶活力的测定

何 欣1,杨 柳2,龚莉君1,杨中侠1   

  1. (1植物病虫害生物学与防控湖南省重点实验室,湖南农业大学植物保护学院,长沙 410128;2长沙市烟草公司宁乡县分公司,湖南宁乡 410600)
  • 收稿日期:2015-11-09 修回日期:2016-02-28 接受日期:2015-12-25 出版日期:2016-03-18 发布日期:2016-03-18
  • 通讯作者: 杨中侠
  • 基金资助:
    湖南省长沙市烟草专卖局科技项目“烟蚜、斜纹夜蛾抗药性快速检测及其综合治理技术”。

Determination of Activities of Three Protective Enzymes from Spodoptera litura (Fabricius) in Ningxiang

He Xin1, Yang Liu2, Gong Lijun1, Yang Zhongxia1   

  1. (1Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, College of Plant Protection, Hunan Agricultural University, Changsha 410128; 2Branch Company of Ningxiang County, Changsha Tobacco Company, Ningxiang Hunan 410600)
  • Received:2015-11-09 Revised:2016-02-28 Accepted:2015-12-25 Online:2016-03-18 Published:2016-03-18

摘要: 为明确斜纹夜蛾幼虫中保护酶对常用药剂抗药性的作用,对湖南宁乡地区斜纹夜蛾3龄幼虫保护酶的活性进行了测定。结果表明:田间种群3种保护酶即过氧化氢酶(CAT)、超氧化物歧化酶(SOD)及过氧化物酶(POD)的活性均高于室内敏感种群,分别为敏感种群的1.24、1.15及2.11倍,2个种群的SOD及POD活性差异极显著。研究结果说明保护酶尤其SOD及POD在斜纹夜蛾上对杀虫剂的抗性起了重要作用。

关键词: 茶叶, 茶叶, 冠层温度, 气象因子, 霜冻, 评估

Abstract: In order to clarify the role of protective enzymes in the resistance to commonly used insecticides, activities of three protective enzymes from Spodoptera litura (Fabricius) larvae in Ningxiang area were determined. The result showed that activities of the three protective enzymes of the field population were significantly higher than that of the sensitive population in lab. Activities of CAT, SOD and POD of the field population were 1.24, 1.15 and 2.11 times as much as that of the sensitive population, respectively. And activities of SOD and POD of the field population had significant differences compared with that of the sensitive population. The results indicated that the protective enzymes, especially SOD and POD, played important roles in resistance to insecticides in Ningxiang area.